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Molecular epidemiology of avian pathogenic Escherichia coli (APEC) isolated from colisepticemia in poultry.
Vet Microbiol. 2004 Nov 30; 104(1-2):91-101.VM

Abstract

The molecular biology and epidemiology of 150 avian pathogenic Escherichia coli strains (APEC) isolated from septicemic poultry in Germany was investigated by serotyping, pulsed field gel electrophoresis (PFGE), and polymerase chain reaction (PCR). Only 49.6% of the isolates could be grouped to serogroups O1, O2, and O78. Macrorestriction analyses data revealed two large clonal groups (clusters I and II) among the APEC strains with a similarity of 60.9% to each other. An association between restriction pattern and serogroup or origin of the strains was only present in a few subgroups of each clusters I and II, but was not evident. In contrast, our data revealed distinct combinations of virulence-associated genes in that 51.2% of the O2-strains harboured a combination of the genes fyuA, irp2, iucD, tsh, vat, fimC, and colV and 36.4% of the O78-strains possessed the same gene combination with exception of vat. With 34 different gene combinations the non-O1, -O2, -O78 isolates revealed a higher variability in their virulence gene pattern than O1-, O2-, and O78-strains with 6, 13, and 9 patterns, respectively. Our data indicate only a limited association between the virulence gene pattern and the serogroup of APEC strains and question the sensitivity of O-typing for APEC identification without the application of further diagnostic tools. Although a limited number of APEC clones exist, horizontal gene transfer seems to be common in these pathogens. These findings strengthen further research on the population structure of APEC and may be the reason for the lack of clear definition of this common E. coli pathotype.

Authors+Show Affiliations

Institut für Mikrobiologie und Tierseuchen, Freie Universität Berlin, Philippstrasse 13, 10115 Berlin, Germany. ewers.christa@vetmed.fu-berlin.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15530743

Citation

Ewers, Christa, et al. "Molecular Epidemiology of Avian Pathogenic Escherichia Coli (APEC) Isolated From Colisepticemia in Poultry." Veterinary Microbiology, vol. 104, no. 1-2, 2004, pp. 91-101.
Ewers C, Janssen T, Kiessling S, et al. Molecular epidemiology of avian pathogenic Escherichia coli (APEC) isolated from colisepticemia in poultry. Vet Microbiol. 2004;104(1-2):91-101.
Ewers, C., Janssen, T., Kiessling, S., Philipp, H. C., & Wieler, L. H. (2004). Molecular epidemiology of avian pathogenic Escherichia coli (APEC) isolated from colisepticemia in poultry. Veterinary Microbiology, 104(1-2), 91-101.
Ewers C, et al. Molecular Epidemiology of Avian Pathogenic Escherichia Coli (APEC) Isolated From Colisepticemia in Poultry. Vet Microbiol. 2004 Nov 30;104(1-2):91-101. PubMed PMID: 15530743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology of avian pathogenic Escherichia coli (APEC) isolated from colisepticemia in poultry. AU - Ewers,Christa, AU - Janssen,Traute, AU - Kiessling,Sabine, AU - Philipp,Hans-C, AU - Wieler,Lothar H, PY - 2004/04/28/received PY - 2004/09/02/revised PY - 2004/09/02/accepted PY - 2004/11/9/pubmed PY - 2005/1/12/medline PY - 2004/11/9/entrez SP - 91 EP - 101 JF - Veterinary microbiology JO - Vet Microbiol VL - 104 IS - 1-2 N2 - The molecular biology and epidemiology of 150 avian pathogenic Escherichia coli strains (APEC) isolated from septicemic poultry in Germany was investigated by serotyping, pulsed field gel electrophoresis (PFGE), and polymerase chain reaction (PCR). Only 49.6% of the isolates could be grouped to serogroups O1, O2, and O78. Macrorestriction analyses data revealed two large clonal groups (clusters I and II) among the APEC strains with a similarity of 60.9% to each other. An association between restriction pattern and serogroup or origin of the strains was only present in a few subgroups of each clusters I and II, but was not evident. In contrast, our data revealed distinct combinations of virulence-associated genes in that 51.2% of the O2-strains harboured a combination of the genes fyuA, irp2, iucD, tsh, vat, fimC, and colV and 36.4% of the O78-strains possessed the same gene combination with exception of vat. With 34 different gene combinations the non-O1, -O2, -O78 isolates revealed a higher variability in their virulence gene pattern than O1-, O2-, and O78-strains with 6, 13, and 9 patterns, respectively. Our data indicate only a limited association between the virulence gene pattern and the serogroup of APEC strains and question the sensitivity of O-typing for APEC identification without the application of further diagnostic tools. Although a limited number of APEC clones exist, horizontal gene transfer seems to be common in these pathogens. These findings strengthen further research on the population structure of APEC and may be the reason for the lack of clear definition of this common E. coli pathotype. SN - 0378-1135 UR - https://www.unboundmedicine.com/medline/citation/15530743/Molecular_epidemiology_of_avian_pathogenic_Escherichia_coli__APEC__isolated_from_colisepticemia_in_poultry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(04)00331-1 DB - PRIME DP - Unbound Medicine ER -