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Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization.
Biosens Bioelectron. 2004 Nov 15; 20(5):995-1000.BB

Abstract

Carbon-nanotube-modified glassy carbon (CNT/GC) transducers have been developed for enhancing the sensitivity and stability of enzyme-based electrochemical bioassays of DNA hybridization. The amplified signal reflects the interfacial accumulation of phenolic products of the alkaline-phosphatase tracer onto the CNT layer. In particular, chronopotentiometric measurements (following short accumulation periods) offer a substantial enhancement of the response of enzymatically liberated alpha-naphthol. The CNT modifier also leads to a dramatic improvement in the stability of the amperometric response of alpha-naphthol. These advantages of CNT/GC electrodes are illustrated from comparison to unmodified glassy carbon electrodes. Factors influencing the adsorptive accumulation of alpha-naphthol, and the overall performance of the new DNA assay, are assessed and optimized. The attractive performance characteristics of the new multi-amplification electrochemical detection of DNA hybridization are reported in connection to the detection of nucleic acid sequences related to the breast cancer BRCA1 gene.

Authors+Show Affiliations

Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA. joewang@nmsu.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15530796

Citation

Wang, Joseph, et al. "Carbon-nanotube-modified Electrodes for Amplified Enzyme-based Electrical Detection of DNA Hybridization." Biosensors & Bioelectronics, vol. 20, no. 5, 2004, pp. 995-1000.
Wang J, Kawde AN, Jan MR. Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization. Biosens Bioelectron. 2004;20(5):995-1000.
Wang, J., Kawde, A. N., & Jan, M. R. (2004). Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization. Biosensors & Bioelectronics, 20(5), 995-1000.
Wang J, Kawde AN, Jan MR. Carbon-nanotube-modified Electrodes for Amplified Enzyme-based Electrical Detection of DNA Hybridization. Biosens Bioelectron. 2004 Nov 15;20(5):995-1000. PubMed PMID: 15530796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization. AU - Wang,Joseph, AU - Kawde,Abdel-Nasser, AU - Jan,M Rasul, PY - 2004/11/9/pubmed PY - 2005/4/13/medline PY - 2004/11/9/entrez SP - 995 EP - 1000 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 20 IS - 5 N2 - Carbon-nanotube-modified glassy carbon (CNT/GC) transducers have been developed for enhancing the sensitivity and stability of enzyme-based electrochemical bioassays of DNA hybridization. The amplified signal reflects the interfacial accumulation of phenolic products of the alkaline-phosphatase tracer onto the CNT layer. In particular, chronopotentiometric measurements (following short accumulation periods) offer a substantial enhancement of the response of enzymatically liberated alpha-naphthol. The CNT modifier also leads to a dramatic improvement in the stability of the amperometric response of alpha-naphthol. These advantages of CNT/GC electrodes are illustrated from comparison to unmodified glassy carbon electrodes. Factors influencing the adsorptive accumulation of alpha-naphthol, and the overall performance of the new DNA assay, are assessed and optimized. The attractive performance characteristics of the new multi-amplification electrochemical detection of DNA hybridization are reported in connection to the detection of nucleic acid sequences related to the breast cancer BRCA1 gene. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/15530796/Carbon_nanotube_modified_electrodes_for_amplified_enzyme_based_electrical_detection_of_DNA_hybridization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956566304002660 DB - PRIME DP - Unbound Medicine ER -