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Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization.
Biosens Bioelectron. 2004 Nov 15; 20(5):995-1000.BB

Abstract

Carbon-nanotube-modified glassy carbon (CNT/GC) transducers have been developed for enhancing the sensitivity and stability of enzyme-based electrochemical bioassays of DNA hybridization. The amplified signal reflects the interfacial accumulation of phenolic products of the alkaline-phosphatase tracer onto the CNT layer. In particular, chronopotentiometric measurements (following short accumulation periods) offer a substantial enhancement of the response of enzymatically liberated alpha-naphthol. The CNT modifier also leads to a dramatic improvement in the stability of the amperometric response of alpha-naphthol. These advantages of CNT/GC electrodes are illustrated from comparison to unmodified glassy carbon electrodes. Factors influencing the adsorptive accumulation of alpha-naphthol, and the overall performance of the new DNA assay, are assessed and optimized. The attractive performance characteristics of the new multi-amplification electrochemical detection of DNA hybridization are reported in connection to the detection of nucleic acid sequences related to the breast cancer BRCA1 gene.

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Authors+Show Affiliations

Wang J
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, NM 88003, USA. joewang@nmsu.edu
Kawde AN
No affiliation info available
Jan MR
No affiliation info available

MeSH

Alkaline PhosphataseBiosensing TechniquesCoated Materials, BiocompatibleDNAElectrochemistryEquipment DesignEquipment Failure AnalysisIn Situ HybridizationMaterials TestingMicroelectrodesNanotubes, Carbon

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15530796

Citation

Wang, Joseph, et al. "Carbon-nanotube-modified Electrodes for Amplified Enzyme-based Electrical Detection of DNA Hybridization." Biosensors & Bioelectronics, vol. 20, no. 5, 2004, pp. 995-1000.
Wang J, Kawde AN, Jan MR. Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization. Biosens Bioelectron. 2004;20(5):995-1000.
Wang, J., Kawde, A. N., & Jan, M. R. (2004). Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization. Biosensors & Bioelectronics, 20(5), 995-1000.
Wang J, Kawde AN, Jan MR. Carbon-nanotube-modified Electrodes for Amplified Enzyme-based Electrical Detection of DNA Hybridization. Biosens Bioelectron. 2004 Nov 15;20(5):995-1000. PubMed PMID: 15530796.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbon-nanotube-modified electrodes for amplified enzyme-based electrical detection of DNA hybridization. AU - Wang,Joseph, AU - Kawde,Abdel-Nasser, AU - Jan,M Rasul, PY - 2004/11/9/pubmed PY - 2005/4/13/medline PY - 2004/11/9/entrez SP - 995 EP - 1000 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 20 IS - 5 N2 - Carbon-nanotube-modified glassy carbon (CNT/GC) transducers have been developed for enhancing the sensitivity and stability of enzyme-based electrochemical bioassays of DNA hybridization. The amplified signal reflects the interfacial accumulation of phenolic products of the alkaline-phosphatase tracer onto the CNT layer. In particular, chronopotentiometric measurements (following short accumulation periods) offer a substantial enhancement of the response of enzymatically liberated alpha-naphthol. The CNT modifier also leads to a dramatic improvement in the stability of the amperometric response of alpha-naphthol. These advantages of CNT/GC electrodes are illustrated from comparison to unmodified glassy carbon electrodes. Factors influencing the adsorptive accumulation of alpha-naphthol, and the overall performance of the new DNA assay, are assessed and optimized. The attractive performance characteristics of the new multi-amplification electrochemical detection of DNA hybridization are reported in connection to the detection of nucleic acid sequences related to the breast cancer BRCA1 gene. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/15530796/Carbon_nanotube_modified_electrodes_for_amplified_enzyme_based_electrical_detection_of_DNA_hybridization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956566304002660 DB - PRIME DP - Unbound Medicine ER -