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Metabolic regulation of the glucose-6-phosphate dehydrogenase from Paracoccus denitrifcans grown on glucose/nitrate.
Arch Microbiol. 1977 Mar 01; 112(2):225-7.AM

Abstract

Glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate : NADP+ L-oxidoreductase EC 1.1.1.49) isolated from Paracoccus denitrificans grown on glucose/nitrate exhibits both NAD+-and NADP+- linked activities. Both activities have a pH optimum of pH 9.6 (Glycine/NaOH buffer) and neither demonstrates a Mg2+ requirement. Kinetics for both NAD(P)+ and glucose-6-phosphate were investigated. Phosphoenolpyruvate inhibits both activities in a competitive manner with respect to glucose-6-phosphate. ATP inhibits the NAD+-linked activity competitively with respect to glucose-6-phosphate but has no effect on the NADP+-linked activity. Neither of the two activities are inhibited by 100 muM NADH but both are inhibited by NADPH. The NAD+-linked activity is far more sensitive to inhibition by NADPH than the NADP+-linked activity.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15531

Citation

Slabas, A R., and F R. Whatley. "Metabolic Regulation of the Glucose-6-phosphate Dehydrogenase From Paracoccus Denitrifcans Grown On Glucose/nitrate." Archives of Microbiology, vol. 112, no. 2, 1977, pp. 225-7.
Slabas AR, Whatley FR. Metabolic regulation of the glucose-6-phosphate dehydrogenase from Paracoccus denitrifcans grown on glucose/nitrate. Arch Microbiol. 1977;112(2):225-7.
Slabas, A. R., & Whatley, F. R. (1977). Metabolic regulation of the glucose-6-phosphate dehydrogenase from Paracoccus denitrifcans grown on glucose/nitrate. Archives of Microbiology, 112(2), 225-7.
Slabas AR, Whatley FR. Metabolic Regulation of the Glucose-6-phosphate Dehydrogenase From Paracoccus Denitrifcans Grown On Glucose/nitrate. Arch Microbiol. 1977 Mar 1;112(2):225-7. PubMed PMID: 15531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic regulation of the glucose-6-phosphate dehydrogenase from Paracoccus denitrifcans grown on glucose/nitrate. AU - Slabas,A R, AU - Whatley,F R, PY - 1977/3/1/pubmed PY - 1977/3/1/medline PY - 1977/3/1/entrez SP - 225 EP - 7 JF - Archives of microbiology JO - Arch. Microbiol. VL - 112 IS - 2 N2 - Glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate : NADP+ L-oxidoreductase EC 1.1.1.49) isolated from Paracoccus denitrificans grown on glucose/nitrate exhibits both NAD+-and NADP+- linked activities. Both activities have a pH optimum of pH 9.6 (Glycine/NaOH buffer) and neither demonstrates a Mg2+ requirement. Kinetics for both NAD(P)+ and glucose-6-phosphate were investigated. Phosphoenolpyruvate inhibits both activities in a competitive manner with respect to glucose-6-phosphate. ATP inhibits the NAD+-linked activity competitively with respect to glucose-6-phosphate but has no effect on the NADP+-linked activity. Neither of the two activities are inhibited by 100 muM NADH but both are inhibited by NADPH. The NAD+-linked activity is far more sensitive to inhibition by NADPH than the NADP+-linked activity. SN - 0302-8933 UR - https://www.unboundmedicine.com/medline/citation/15531/Metabolic_regulation_of_the_glucose_6_phosphate_dehydrogenase_from_Paracoccus_denitrifcans_grown_on_glucose/nitrate_ DB - PRIME DP - Unbound Medicine ER -