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The fungal STRE-element-binding protein Seb1 is involved but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in Trichoderma atroviride during osmotic stress.
Fungal Genet Biol. 2004 Dec; 41(12):1132-40.FG

Abstract

Fungi counteract extracellular osmotic pressure by producing intracellular polyols to prevent loss of water. In yeast osmotic signaling involves a MAP-kinase pathway culminating at the STRE-binding transcription factors Msn2/4. We investigated the role of a putative STRE-binding orthologue of Trichoderma atroviride, Seb1, in osmotic stress signaling. T. atroviride, subjected to osmotic stress (10% glucose or glycerol, 1M KCl or NaCl), responds by raising its intracellular glycerol level. In contrast to Aspergillus nidulans, no erythritol is accumulated. Accumulation of glycerol levels under osmotic stress is strongly reduced in a seb1 deletion strain. To investigate glycerol biosynthesis in T. atroviride, the genes encoding glycerol dehydrogenase (gld1) and glycerol-3-phosphate dehydrogenase (gfd1) were cloned and characterized. Although both genes contain STRE-elements in their 5'-non-coding regions, only gld1 mRNA accumulates in response to osmotic stress, whereas expression of gfd1 remains at a constitutive level. In comparison to A. nidulans gld1 transcript levels in T. atroviride rise very slowly under conditions of salt stress. Deletion of seb1 results in a delayed accumulation of the gld1 transcript, but final levels match those in the wild-type whereas gfd1 transcript accumulation remains unaffected. Assays for glycerol dehydrogenase and glycerol-3-phosphate dehydrogenase enzymatic activities reveal an increase of the former--whereas the latter remains mainly unaffected--in the wild-type and the Deltaseb1 strain under different kinds of osmotic stress. The data suggest that Seb1 is only involved in, but not essential for osmotic stress response which is in contrast to the yeast orthologues Msn2/4.

Authors+Show Affiliations

Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Getreidemarkt 9/166-5, A-1060 Wien, Austria. vseidl@mail.zserv.tuwien.ac.atNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15531216

Citation

Seidl, Verena, et al. "The Fungal STRE-element-binding Protein Seb1 Is Involved but Not Essential for Glycerol Dehydrogenase (gld1) Gene Expression and Glycerol Accumulation in Trichoderma Atroviride During Osmotic Stress." Fungal Genetics and Biology : FG & B, vol. 41, no. 12, 2004, pp. 1132-40.
Seidl V, Seiboth B, Karaffa L, et al. The fungal STRE-element-binding protein Seb1 is involved but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in Trichoderma atroviride during osmotic stress. Fungal Genet Biol. 2004;41(12):1132-40.
Seidl, V., Seiboth, B., Karaffa, L., & Kubicek, C. P. (2004). The fungal STRE-element-binding protein Seb1 is involved but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in Trichoderma atroviride during osmotic stress. Fungal Genetics and Biology : FG & B, 41(12), 1132-40.
Seidl V, et al. The Fungal STRE-element-binding Protein Seb1 Is Involved but Not Essential for Glycerol Dehydrogenase (gld1) Gene Expression and Glycerol Accumulation in Trichoderma Atroviride During Osmotic Stress. Fungal Genet Biol. 2004;41(12):1132-40. PubMed PMID: 15531216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The fungal STRE-element-binding protein Seb1 is involved but not essential for glycerol dehydrogenase (gld1) gene expression and glycerol accumulation in Trichoderma atroviride during osmotic stress. AU - Seidl,Verena, AU - Seiboth,Bernhard, AU - Karaffa,Levente, AU - Kubicek,Christian P, PY - 2004/06/02/received PY - 2004/09/07/accepted PY - 2004/11/9/pubmed PY - 2005/5/10/medline PY - 2004/11/9/entrez SP - 1132 EP - 40 JF - Fungal genetics and biology : FG & B JO - Fungal Genet Biol VL - 41 IS - 12 N2 - Fungi counteract extracellular osmotic pressure by producing intracellular polyols to prevent loss of water. In yeast osmotic signaling involves a MAP-kinase pathway culminating at the STRE-binding transcription factors Msn2/4. We investigated the role of a putative STRE-binding orthologue of Trichoderma atroviride, Seb1, in osmotic stress signaling. T. atroviride, subjected to osmotic stress (10% glucose or glycerol, 1M KCl or NaCl), responds by raising its intracellular glycerol level. In contrast to Aspergillus nidulans, no erythritol is accumulated. Accumulation of glycerol levels under osmotic stress is strongly reduced in a seb1 deletion strain. To investigate glycerol biosynthesis in T. atroviride, the genes encoding glycerol dehydrogenase (gld1) and glycerol-3-phosphate dehydrogenase (gfd1) were cloned and characterized. Although both genes contain STRE-elements in their 5'-non-coding regions, only gld1 mRNA accumulates in response to osmotic stress, whereas expression of gfd1 remains at a constitutive level. In comparison to A. nidulans gld1 transcript levels in T. atroviride rise very slowly under conditions of salt stress. Deletion of seb1 results in a delayed accumulation of the gld1 transcript, but final levels match those in the wild-type whereas gfd1 transcript accumulation remains unaffected. Assays for glycerol dehydrogenase and glycerol-3-phosphate dehydrogenase enzymatic activities reveal an increase of the former--whereas the latter remains mainly unaffected--in the wild-type and the Deltaseb1 strain under different kinds of osmotic stress. The data suggest that Seb1 is only involved in, but not essential for osmotic stress response which is in contrast to the yeast orthologues Msn2/4. SN - 1087-1845 UR - https://www.unboundmedicine.com/medline/citation/15531216/The_fungal_STRE_element_binding_protein_Seb1_is_involved_but_not_essential_for_glycerol_dehydrogenase__gld1__gene_expression_and_glycerol_accumulation_in_Trichoderma_atroviride_during_osmotic_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1087-1845(04)00140-9 DB - PRIME DP - Unbound Medicine ER -