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Determination of rosiglitazone in coated tablets by MEKC and HPLC methods.
J Pharm Biomed Anal. 2004 Nov 19; 36(4):909-13.JP

Abstract

Micellar electrokinetic chromatographic (MEKC) and high-performance liquid chromatographic (HPLC) methods were developed and subsequently validated for the determination of rosiglitazone (RSG) in coated tablet, a potent new oral antihyperglicemic agent. The electrophoretic separation was performed in a fused-silica capillary of total length 48.0 cm (effective length 39.5 cm, 75 microm i.d.) using 10 mM sodium tetraborate buffer (pH 9.0) containing 30 mM sodium dodecyl sulfate (SDS) as the background electrolyte (BGE). The separating voltage used was of 20 kV at 25 degrees C and the diode array detector was set at 247 nm. The MEKC method was compared with HPLC method using a RP-18 column (125 x 4.0mm i.d.) eluted with a mobile phase consisting of mixture of 25 mM potassium dihydrogen phosphate buffer and acetonitrile (55:45, v/v), adjusting the pH to 6.2 with dilute potassium hydroxide. Statistical analysis by Student's t-test showed no significant differences between the results obtained by two methods. The results indicated that MEKC can be used an alternative method to HPLC for the determination of rosiglitazone in pharmaceutical dosage form.

Authors+Show Affiliations

Gomes P
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752 Lab.402, Porto Alegre 90610-000, RS, Brazil. patriciagomes0@yahoo.com.br
Sippel J
No affiliation info available
Jablonski A
No affiliation info available
Steppe M
No affiliation info available

MeSH

Chromatography, High Pressure LiquidChromatography, Micellar Electrokinetic CapillaryRosiglitazoneTablets, Enteric-CoatedThiazolidinediones

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15533689

Citation

Gomes, Patrícia, et al. "Determination of Rosiglitazone in Coated Tablets By MEKC and HPLC Methods." Journal of Pharmaceutical and Biomedical Analysis, vol. 36, no. 4, 2004, pp. 909-13.
Gomes P, Sippel J, Jablonski A, et al. Determination of rosiglitazone in coated tablets by MEKC and HPLC methods. J Pharm Biomed Anal. 2004;36(4):909-13.
Gomes, P., Sippel, J., Jablonski, A., & Steppe, M. (2004). Determination of rosiglitazone in coated tablets by MEKC and HPLC methods. Journal of Pharmaceutical and Biomedical Analysis, 36(4), 909-13.
Gomes P, et al. Determination of Rosiglitazone in Coated Tablets By MEKC and HPLC Methods. J Pharm Biomed Anal. 2004 Nov 19;36(4):909-13. PubMed PMID: 15533689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of rosiglitazone in coated tablets by MEKC and HPLC methods. AU - Gomes,Patrícia, AU - Sippel,Juliana, AU - Jablonski,André, AU - Steppe,Martin, PY - 2004/03/29/received PY - 2004/08/20/revised PY - 2004/08/20/accepted PY - 2004/11/10/pubmed PY - 2005/4/13/medline PY - 2004/11/10/entrez SP - 909 EP - 13 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 36 IS - 4 N2 - Micellar electrokinetic chromatographic (MEKC) and high-performance liquid chromatographic (HPLC) methods were developed and subsequently validated for the determination of rosiglitazone (RSG) in coated tablet, a potent new oral antihyperglicemic agent. The electrophoretic separation was performed in a fused-silica capillary of total length 48.0 cm (effective length 39.5 cm, 75 microm i.d.) using 10 mM sodium tetraborate buffer (pH 9.0) containing 30 mM sodium dodecyl sulfate (SDS) as the background electrolyte (BGE). The separating voltage used was of 20 kV at 25 degrees C and the diode array detector was set at 247 nm. The MEKC method was compared with HPLC method using a RP-18 column (125 x 4.0mm i.d.) eluted with a mobile phase consisting of mixture of 25 mM potassium dihydrogen phosphate buffer and acetonitrile (55:45, v/v), adjusting the pH to 6.2 with dilute potassium hydroxide. Statistical analysis by Student's t-test showed no significant differences between the results obtained by two methods. The results indicated that MEKC can be used an alternative method to HPLC for the determination of rosiglitazone in pharmaceutical dosage form. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/15533689/Determination_of_rosiglitazone_in_coated_tablets_by_MEKC_and_HPLC_methods_ DB - PRIME DP - Unbound Medicine ER -