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Micellar electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine.
J Sep Sci. 2004 Oct; 27(14):1211-4.JS

Abstract

A selective and sensitive micellar electrokinetic chromatography method with laser-induced fluorescence detection was developed for the quantification of ephedrine (E) and pseudoephedrine (PE) derivatized with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. After conducting a series of optimizations, a running buffer of 10 mM sodium borate + 16 mM SDS was used for separation of the derivatives. A linear relationship for E and PE was obtained in the range of 0.044-6.6 microg mL(-1) (correlation coefficient: 0.9943 for E, 0.9946 for PE), and the detection limits for E and PE were 0.70 and 0.30 ng mL(-1), respectively. The sensitivity of E and PE was improved by several multiples of ten over those of CZE-LIF method. The method was applied to the analysis of the two alkaloids in ephedra herbal medicine and preparations with recoveries in the range of 98.3-107.1%.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15537078

Citation

Xie, Jianping, et al. "Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection for Sensitive Determination of Ephedrine and Pseudoephedrine." Journal of Separation Science, vol. 27, no. 14, 2004, pp. 1211-4.
Xie J, Zhang J, Liu J, et al. Micellar electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine. J Sep Sci. 2004;27(14):1211-4.
Xie, J., Zhang, J., Liu, J., Tian, J., Chen, X., & Hu, Z. (2004). Micellar electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine. Journal of Separation Science, 27(14), 1211-4.
Xie J, et al. Micellar Electrokinetic Chromatography With Laser-induced Fluorescence Detection for Sensitive Determination of Ephedrine and Pseudoephedrine. J Sep Sci. 2004;27(14):1211-4. PubMed PMID: 15537078.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micellar electrokinetic chromatography with laser-induced fluorescence detection for sensitive determination of ephedrine and pseudoephedrine. AU - Xie,Jianping, AU - Zhang,Jiyou, AU - Liu,Jiaqin, AU - Tian,Jianniao, AU - Chen,Xingguo, AU - Hu,Zhide, PY - 2004/11/13/pubmed PY - 2005/3/30/medline PY - 2004/11/13/entrez SP - 1211 EP - 4 JF - Journal of separation science JO - J Sep Sci VL - 27 IS - 14 N2 - A selective and sensitive micellar electrokinetic chromatography method with laser-induced fluorescence detection was developed for the quantification of ephedrine (E) and pseudoephedrine (PE) derivatized with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. After conducting a series of optimizations, a running buffer of 10 mM sodium borate + 16 mM SDS was used for separation of the derivatives. A linear relationship for E and PE was obtained in the range of 0.044-6.6 microg mL(-1) (correlation coefficient: 0.9943 for E, 0.9946 for PE), and the detection limits for E and PE were 0.70 and 0.30 ng mL(-1), respectively. The sensitivity of E and PE was improved by several multiples of ten over those of CZE-LIF method. The method was applied to the analysis of the two alkaloids in ephedra herbal medicine and preparations with recoveries in the range of 98.3-107.1%. SN - 1615-9306 UR - https://www.unboundmedicine.com/medline/citation/15537078/Micellar_electrokinetic_chromatography_with_laser_induced_fluorescence_detection_for_sensitive_determination_of_ephedrine_and_pseudoephedrine_ L2 - https://doi.org/10.1002/jssc.200401756 DB - PRIME DP - Unbound Medicine ER -