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In vitro fluconazole susceptibility of 1565 clinical isolates of Candida species evaluated by the disk diffusion method performed using NCCLS M44-A guidelines.
Diagn Microbiol Infect Dis. 2004 Nov; 50(3):187-92.DM

Abstract

We determined the in vitro activity of fluconazole against 1565 clinical Candida spp. isolates collected from different specimens of non-AIDS outpatients and inpatients in 3 different regions of Italy. Susceptibility testing was performed by agar disk diffusion using the NCCLS document M44-A guidelines. Candida albicans was the most frequently isolated yeast (68%) followed by C. glabrata (15%), C. tropicalis (5%), C. parapsilosis (5%), and C. krusei (5%). Other yeasts represented 4% of all isolates. Of the 1565 isolates tested, 1449 (92.6%) were susceptible (S) to fluconazole, 43 (2.7%) were susceptible dose-dependent (S-DD) and 73 (4.7%) were resistant (R). Almost all (98.2%) of the C. albicans isolates were classified as S or S-DD. Despite its widespread use, fluconazole displayed good activity against the isolates we tested, and the disk diffusion method was confirmed as a reliable approach to the evaluation of in vitro susceptibility of yeasts to this antimycotic agent.

Authors+Show Affiliations

University of Rome Tor Vergata, Department of Public Health, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15541604

Citation

Testore, Gian Piero, et al. "In Vitro Fluconazole Susceptibility of 1565 Clinical Isolates of Candida Species Evaluated By the Disk Diffusion Method Performed Using NCCLS M44-A Guidelines." Diagnostic Microbiology and Infectious Disease, vol. 50, no. 3, 2004, pp. 187-92.
Testore GP, Dori L, Buonomini AR, et al. In vitro fluconazole susceptibility of 1565 clinical isolates of Candida species evaluated by the disk diffusion method performed using NCCLS M44-A guidelines. Diagn Microbiol Infect Dis. 2004;50(3):187-92.
Testore, G. P., Dori, L., Buonomini, A. R., Schito, G. C., Soro, O., Fortina, G., Andreoni, S., Carlone, N., Tullio, V., & Andreoni, M. (2004). In vitro fluconazole susceptibility of 1565 clinical isolates of Candida species evaluated by the disk diffusion method performed using NCCLS M44-A guidelines. Diagnostic Microbiology and Infectious Disease, 50(3), 187-92.
Testore GP, et al. In Vitro Fluconazole Susceptibility of 1565 Clinical Isolates of Candida Species Evaluated By the Disk Diffusion Method Performed Using NCCLS M44-A Guidelines. Diagn Microbiol Infect Dis. 2004;50(3):187-92. PubMed PMID: 15541604.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro fluconazole susceptibility of 1565 clinical isolates of Candida species evaluated by the disk diffusion method performed using NCCLS M44-A guidelines. AU - Testore,Gian Piero, AU - Dori,Luca, AU - Buonomini,Anna Rita, AU - Schito,Gian Carlo, AU - Soro,Ornella, AU - Fortina,Giacomo, AU - Andreoni,Stefano, AU - Carlone,Nicola, AU - Tullio,Vivian, AU - Andreoni,Massimo, PY - 2004/05/11/received PY - 2004/07/21/accepted PY - 2004/11/16/pubmed PY - 2005/2/18/medline PY - 2004/11/16/entrez SP - 187 EP - 92 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 50 IS - 3 N2 - We determined the in vitro activity of fluconazole against 1565 clinical Candida spp. isolates collected from different specimens of non-AIDS outpatients and inpatients in 3 different regions of Italy. Susceptibility testing was performed by agar disk diffusion using the NCCLS document M44-A guidelines. Candida albicans was the most frequently isolated yeast (68%) followed by C. glabrata (15%), C. tropicalis (5%), C. parapsilosis (5%), and C. krusei (5%). Other yeasts represented 4% of all isolates. Of the 1565 isolates tested, 1449 (92.6%) were susceptible (S) to fluconazole, 43 (2.7%) were susceptible dose-dependent (S-DD) and 73 (4.7%) were resistant (R). Almost all (98.2%) of the C. albicans isolates were classified as S or S-DD. Despite its widespread use, fluconazole displayed good activity against the isolates we tested, and the disk diffusion method was confirmed as a reliable approach to the evaluation of in vitro susceptibility of yeasts to this antimycotic agent. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/15541604/In_vitro_fluconazole_susceptibility_of_1565_clinical_isolates_of_Candida_species_evaluated_by_the_disk_diffusion_method_performed_using_NCCLS_M44_A_guidelines_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732889304001476 DB - PRIME DP - Unbound Medicine ER -