Tags

Type your tag names separated by a space and hit enter

Application of extended single-reaction multiplex polymerase chain reaction for toxin typing of Staphylococcus aureus isolates in South Korea.
Int J Food Microbiol. 2004 Dec 15; 97(2):137-45.IJ

Abstract

The extended single-reaction multiplex PCR (esr-mPCR) developed in this study to detect staphylococcal enterotoxins (SEs), including SEA, SEB, SEC, SED, SEE, SEH, SEI, and SEJ, requires fewer sets of primers than other conventional multiplex PCRs and can be used to detect newly identified staphylococcal enterotoxins SEs more readily. Esr-mPCR analysis of 141 isolates of Staphylococcus aureus obtained from abattoir and livestock product samples revealed that 27 of the S. aureus isolates were toxigenic, and two were 2 multitoxigenic isolates. The most prevalent SE type was SEI followed by SEA and SEH. In addition, we investigated the clonal relatedness of toxigenic S. aureus isolates by arbitrarily primed PCR (AP-PCR). AP-PCR analysis of toxigenic S. aureus isolates revealed that the discriminatory power of AP-PCR was 9 (D=0.81), 8 (D=0.77), and 10 types (D=0.83) with primers AP1, ERIC2, and AP7, respectively. The combination of three each AP-PCR result could rearrange toxigenic S. aureus isolates into 10 types and five subtypes, with the D-value of 0.92. Interestingly, our data showed that toxigenic S. aureus isolates from different sources had different fingerprinting patterns although some of them carried the same types of SE genes. These data suggest that combinations of esr-mPCR and AP-PCR can provide a powerful approach for epidemiological investigation of toxigenic S. aureus isolates.

Authors+Show Affiliations

Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Shilim 9-dong, Gwanak-gu, Seoul 151-742, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15541800

Citation

Kwon, N H., et al. "Application of Extended Single-reaction Multiplex Polymerase Chain Reaction for Toxin Typing of Staphylococcus Aureus Isolates in South Korea." International Journal of Food Microbiology, vol. 97, no. 2, 2004, pp. 137-45.
Kwon NH, Kim SH, Park KT, et al. Application of extended single-reaction multiplex polymerase chain reaction for toxin typing of Staphylococcus aureus isolates in South Korea. Int J Food Microbiol. 2004;97(2):137-45.
Kwon, N. H., Kim, S. H., Park, K. T., Bae, W. K., Kim, J. Y., Lim, J. Y., Ahn, J. S., Lyoo, K. S., Kim, J. M., Jung, W. K., Noh, K. M., Bohach, G. A., & Park, Y. H. (2004). Application of extended single-reaction multiplex polymerase chain reaction for toxin typing of Staphylococcus aureus isolates in South Korea. International Journal of Food Microbiology, 97(2), 137-45.
Kwon NH, et al. Application of Extended Single-reaction Multiplex Polymerase Chain Reaction for Toxin Typing of Staphylococcus Aureus Isolates in South Korea. Int J Food Microbiol. 2004 Dec 15;97(2):137-45. PubMed PMID: 15541800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of extended single-reaction multiplex polymerase chain reaction for toxin typing of Staphylococcus aureus isolates in South Korea. AU - Kwon,N H, AU - Kim,S H, AU - Park,K T, AU - Bae,W K, AU - Kim,J Y, AU - Lim,J Y, AU - Ahn,J S, AU - Lyoo,K S, AU - Kim,J M, AU - Jung,W K, AU - Noh,K M, AU - Bohach,G A, AU - Park,Y H, PY - 2003/03/10/received PY - 2004/03/31/revised PY - 2004/04/22/accepted PY - 2004/11/16/pubmed PY - 2005/2/3/medline PY - 2004/11/16/entrez SP - 137 EP - 45 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 97 IS - 2 N2 - The extended single-reaction multiplex PCR (esr-mPCR) developed in this study to detect staphylococcal enterotoxins (SEs), including SEA, SEB, SEC, SED, SEE, SEH, SEI, and SEJ, requires fewer sets of primers than other conventional multiplex PCRs and can be used to detect newly identified staphylococcal enterotoxins SEs more readily. Esr-mPCR analysis of 141 isolates of Staphylococcus aureus obtained from abattoir and livestock product samples revealed that 27 of the S. aureus isolates were toxigenic, and two were 2 multitoxigenic isolates. The most prevalent SE type was SEI followed by SEA and SEH. In addition, we investigated the clonal relatedness of toxigenic S. aureus isolates by arbitrarily primed PCR (AP-PCR). AP-PCR analysis of toxigenic S. aureus isolates revealed that the discriminatory power of AP-PCR was 9 (D=0.81), 8 (D=0.77), and 10 types (D=0.83) with primers AP1, ERIC2, and AP7, respectively. The combination of three each AP-PCR result could rearrange toxigenic S. aureus isolates into 10 types and five subtypes, with the D-value of 0.92. Interestingly, our data showed that toxigenic S. aureus isolates from different sources had different fingerprinting patterns although some of them carried the same types of SE genes. These data suggest that combinations of esr-mPCR and AP-PCR can provide a powerful approach for epidemiological investigation of toxigenic S. aureus isolates. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/15541800/Application_of_extended_single_reaction_multiplex_polymerase_chain_reaction_for_toxin_typing_of_Staphylococcus_aureus_isolates_in_South_Korea_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168160504002594 DB - PRIME DP - Unbound Medicine ER -