Tags

Type your tag names separated by a space and hit enter

Elevation of plasminogen activators in cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis.
Int J Parasitol. 2004 Nov; 34(12):1355-64.IJ

Abstract

A hallmark of parasitic meningitis is the infiltration of eosinophils into the subarachnoid space. Infection with Angiostrongylus cantonensis in mice induced proteinase activity in parallel with the pathological changes of eosinophilic meningitis. Zymogram analysis demonstrated that 70 and 55 kDa proteinases from cerebrospinal fluid (CSF) were active against the casein/plasminogen substrate. The proteinase activities were clearly inhibited by phenylmethanesulphonyl fluoride but not by ethylenediamine tetraacetic acid, 1,10-phenanthroline or leupeptin. Western blotting confirmed these enzymes to be tissue-type plasminogen activator and urokinase-type plasminogen activator, respectively. High activities of tissue-type plasminogen activator and urokinase-type plasminogen activator were detected in the CSF of mice with eosinophilic meningitis, and correlated positively with CSF eosinophil numbers and total protein, respectively. Immunohistochemistry demonstrated that tissue-type plasminogen activator and urokinase-type plasminogen activator localised in the endothelial cells of blood vessels, in blood clots and in infiltrated leukocytes. These results suggest that tissue-type plasminogen activator and urokinase-type plasminogen activator may be play a role in the pathogenesis of eosinophilic meningitis of angiostrongyliasis.

Authors+Show Affiliations

Department of Entomology, National Chung-Hsing University, Taichung 402, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15542096

Citation

Hou, Roger F., et al. "Elevation of Plasminogen Activators in Cerebrospinal Fluid of Mice With Eosinophilic Meningitis Caused By Angiostrongylus Cantonensis." International Journal for Parasitology, vol. 34, no. 12, 2004, pp. 1355-64.
Hou RF, Tu WC, Lee HH, et al. Elevation of plasminogen activators in cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis. Int J Parasitol. 2004;34(12):1355-64.
Hou, R. F., Tu, W. C., Lee, H. H., Chen, K. M., Chou, H. L., & Lai, S. C. (2004). Elevation of plasminogen activators in cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis. International Journal for Parasitology, 34(12), 1355-64.
Hou RF, et al. Elevation of Plasminogen Activators in Cerebrospinal Fluid of Mice With Eosinophilic Meningitis Caused By Angiostrongylus Cantonensis. Int J Parasitol. 2004;34(12):1355-64. PubMed PMID: 15542096.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Elevation of plasminogen activators in cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis. AU - Hou,Roger F, AU - Tu,Wu-Chun, AU - Lee,Hsiu-Hsiung, AU - Chen,Ke-Min, AU - Chou,Hui-Lin, AU - Lai,Shih-Chan, PY - 2004/07/23/received PY - 2004/08/25/revised PY - 2004/08/27/accepted PY - 2004/11/16/pubmed PY - 2005/3/3/medline PY - 2004/11/16/entrez SP - 1355 EP - 64 JF - International journal for parasitology JO - Int J Parasitol VL - 34 IS - 12 N2 - A hallmark of parasitic meningitis is the infiltration of eosinophils into the subarachnoid space. Infection with Angiostrongylus cantonensis in mice induced proteinase activity in parallel with the pathological changes of eosinophilic meningitis. Zymogram analysis demonstrated that 70 and 55 kDa proteinases from cerebrospinal fluid (CSF) were active against the casein/plasminogen substrate. The proteinase activities were clearly inhibited by phenylmethanesulphonyl fluoride but not by ethylenediamine tetraacetic acid, 1,10-phenanthroline or leupeptin. Western blotting confirmed these enzymes to be tissue-type plasminogen activator and urokinase-type plasminogen activator, respectively. High activities of tissue-type plasminogen activator and urokinase-type plasminogen activator were detected in the CSF of mice with eosinophilic meningitis, and correlated positively with CSF eosinophil numbers and total protein, respectively. Immunohistochemistry demonstrated that tissue-type plasminogen activator and urokinase-type plasminogen activator localised in the endothelial cells of blood vessels, in blood clots and in infiltrated leukocytes. These results suggest that tissue-type plasminogen activator and urokinase-type plasminogen activator may be play a role in the pathogenesis of eosinophilic meningitis of angiostrongyliasis. SN - 0020-7519 UR - https://www.unboundmedicine.com/medline/citation/15542096/Elevation_of_plasminogen_activators_in_cerebrospinal_fluid_of_mice_with_eosinophilic_meningitis_caused_by_Angiostrongylus_cantonensis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0020-7519(04)00187-0 DB - PRIME DP - Unbound Medicine ER -