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U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction.
Nat Struct Mol Biol. 2004 Dec; 11(12):1237-42.NS

Abstract

Intron removal in nuclear precursor mRNA is catalyzed through two transesterification reactions by a multi-megaDalton ribonucleoprotein machine called the spliceosome. A complex between U2 and U6 small nuclear RNAs is a core component of the spliceosome. Here we present an NMR structural analysis of a protein-free U2-U6 complex from Saccharomyces cerevisiae. The observed folding of the U2-U6 complex is a four-helix junction, in which the catalytically important AGC triad base-pairs only within U6 and not with U2. The base-pairing of the AGC triad extends the U6 intramolecular stem-loop (U6 ISL), and the NMR structure of this extended U6 ISL reveals structural similarities with domain 5 of group II self-splicing introns. The observed conformation of the four-helix junction could be relevant to the first, but not the second, step of splicing and may help to position the U6 ISL adjacent to the 5' splice site.

Authors+Show Affiliations

Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15543154

Citation

Sashital, Dipali G., et al. "U2-U6 RNA Folding Reveals a Group II Intron-like Domain and a Four-helix Junction." Nature Structural & Molecular Biology, vol. 11, no. 12, 2004, pp. 1237-42.
Sashital DG, Cornilescu G, McManus CJ, et al. U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction. Nat Struct Mol Biol. 2004;11(12):1237-42.
Sashital, D. G., Cornilescu, G., McManus, C. J., Brow, D. A., & Butcher, S. E. (2004). U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction. Nature Structural & Molecular Biology, 11(12), 1237-42.
Sashital DG, et al. U2-U6 RNA Folding Reveals a Group II Intron-like Domain and a Four-helix Junction. Nat Struct Mol Biol. 2004;11(12):1237-42. PubMed PMID: 15543154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - U2-U6 RNA folding reveals a group II intron-like domain and a four-helix junction. AU - Sashital,Dipali G, AU - Cornilescu,Gabriel, AU - McManus,C Joel, AU - Brow,David A, AU - Butcher,Samuel E, Y1 - 2004/11/14/ PY - 2004/08/12/received PY - 2004/09/30/accepted PY - 2004/11/16/pubmed PY - 2005/1/7/medline PY - 2004/11/16/entrez SP - 1237 EP - 42 JF - Nature structural & molecular biology JO - Nat Struct Mol Biol VL - 11 IS - 12 N2 - Intron removal in nuclear precursor mRNA is catalyzed through two transesterification reactions by a multi-megaDalton ribonucleoprotein machine called the spliceosome. A complex between U2 and U6 small nuclear RNAs is a core component of the spliceosome. Here we present an NMR structural analysis of a protein-free U2-U6 complex from Saccharomyces cerevisiae. The observed folding of the U2-U6 complex is a four-helix junction, in which the catalytically important AGC triad base-pairs only within U6 and not with U2. The base-pairing of the AGC triad extends the U6 intramolecular stem-loop (U6 ISL), and the NMR structure of this extended U6 ISL reveals structural similarities with domain 5 of group II self-splicing introns. The observed conformation of the four-helix junction could be relevant to the first, but not the second, step of splicing and may help to position the U6 ISL adjacent to the 5' splice site. SN - 1545-9993 UR - https://www.unboundmedicine.com/medline/citation/15543154/U2_U6_RNA_folding_reveals_a_group_II_intron_like_domain_and_a_four_helix_junction_ L2 - https://doi.org/10.1038/nsmb863 DB - PRIME DP - Unbound Medicine ER -