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Multiple drug resistance protein (MDR-1), multidrug resistance-related protein (MRP) and lung resistance protein (LRP) gene expression in childhood acute lymphoblastic leukemia.
Sao Paulo Med J. 2004 Jul 01; 122(4):166-71.SP

Abstract

CONTEXT

Despite the advances in the cure rate for acute lymphoblastic leukemia, approximately 25% of affected children suffer relapses. Expression of genes for the multiple drug resistance protein (MDR-1), multidrug resistance-related protein (MRP), and lung resistance protein (LRP) may confer the phenotype of resistance to the treatment of neoplasias.

OBJECTIVE

To analyze the expression of the MDR-1, MRP and LRP genes in children with a diagnosis of acute lymphoblastic leukemia via the semiquantitative reverse transcription polymerase chain reaction (RT-PCR), and to determine the correlation between expression and event-free survival and clinical and laboratory variables.

DESIGN

A retrospective clinical study.

SETTING

Laboratory of Pediatric Oncology, Department of Pediatrics, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil.

METHODS

Bone marrow aspirates from 30 children with a diagnosis of acute lymphoblastic leukemia were assessed for the expression of messenger RNA for the MDR-1, MRP and LRP genes by semi-quantitative RT-PCR.

RESULTS

In the three groups studied, only the increased expression of LRP was related to worsened event-free survival (p = 0.005). The presence of the common acute lymphoblastic leukemia antigen (CALLA) was correlated with increased LRP expression (p = 0.009) and increased risk of relapse or death (p = 0.05). The relative risk of relapse or death was six times higher among children with high LRP expression upon diagnosis (p = 0.05), as confirmed by multivariate analysis of the three genes studied (p = 0.035).

DISCUSSION

Cell resistance to drugs is a determinant of the response to chemotherapy and its detection via RT-PCR may be of clinical importance.

CONCLUSIONS

Evaluation of the expression of genes for resistance to antineoplastic drugs in childhood acute lymphoblastic leukemia upon diagnosis, and particularly the expression of the LRP gene, may be of clinical relevance, and should be the object of prospective studies.

Authors+Show Affiliations

Laboratory of Pediatric Oncology, Department of Pediatrics, University Hospital, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. elvisvalera@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

15543372

Citation

Valera, Elvis Terci, et al. "Multiple Drug Resistance Protein (MDR-1), Multidrug Resistance-related Protein (MRP) and Lung Resistance Protein (LRP) Gene Expression in Childhood Acute Lymphoblastic Leukemia." Sao Paulo Medical Journal = Revista Paulista De Medicina, vol. 122, no. 4, 2004, pp. 166-71.
Valera ET, Scrideli CA, Queiroz RG, et al. Multiple drug resistance protein (MDR-1), multidrug resistance-related protein (MRP) and lung resistance protein (LRP) gene expression in childhood acute lymphoblastic leukemia. Sao Paulo Med J. 2004;122(4):166-71.
Valera, E. T., Scrideli, C. A., Queiroz, R. G., Mori, B. M., & Tone, L. G. (2004). Multiple drug resistance protein (MDR-1), multidrug resistance-related protein (MRP) and lung resistance protein (LRP) gene expression in childhood acute lymphoblastic leukemia. Sao Paulo Medical Journal = Revista Paulista De Medicina, 122(4), 166-71.
Valera ET, et al. Multiple Drug Resistance Protein (MDR-1), Multidrug Resistance-related Protein (MRP) and Lung Resistance Protein (LRP) Gene Expression in Childhood Acute Lymphoblastic Leukemia. Sao Paulo Med J. 2004 Jul 1;122(4):166-71. PubMed PMID: 15543372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple drug resistance protein (MDR-1), multidrug resistance-related protein (MRP) and lung resistance protein (LRP) gene expression in childhood acute lymphoblastic leukemia. AU - Valera,Elvis Terci, AU - Scrideli,Carlos Alberto, AU - Queiroz,Rosane Gomes de Paula, AU - Mori,Bianca Maria Ortelli, AU - Tone,Luiz Gonzaga, Y1 - 2004/11/09/ PY - 2004/11/16/pubmed PY - 2005/4/29/medline PY - 2004/11/16/entrez SP - 166 EP - 71 JF - Sao Paulo medical journal = Revista paulista de medicina JO - Sao Paulo Med J VL - 122 IS - 4 N2 - CONTEXT: Despite the advances in the cure rate for acute lymphoblastic leukemia, approximately 25% of affected children suffer relapses. Expression of genes for the multiple drug resistance protein (MDR-1), multidrug resistance-related protein (MRP), and lung resistance protein (LRP) may confer the phenotype of resistance to the treatment of neoplasias. OBJECTIVE: To analyze the expression of the MDR-1, MRP and LRP genes in children with a diagnosis of acute lymphoblastic leukemia via the semiquantitative reverse transcription polymerase chain reaction (RT-PCR), and to determine the correlation between expression and event-free survival and clinical and laboratory variables. DESIGN: A retrospective clinical study. SETTING: Laboratory of Pediatric Oncology, Department of Pediatrics, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil. METHODS: Bone marrow aspirates from 30 children with a diagnosis of acute lymphoblastic leukemia were assessed for the expression of messenger RNA for the MDR-1, MRP and LRP genes by semi-quantitative RT-PCR. RESULTS: In the three groups studied, only the increased expression of LRP was related to worsened event-free survival (p = 0.005). The presence of the common acute lymphoblastic leukemia antigen (CALLA) was correlated with increased LRP expression (p = 0.009) and increased risk of relapse or death (p = 0.05). The relative risk of relapse or death was six times higher among children with high LRP expression upon diagnosis (p = 0.05), as confirmed by multivariate analysis of the three genes studied (p = 0.035). DISCUSSION: Cell resistance to drugs is a determinant of the response to chemotherapy and its detection via RT-PCR may be of clinical importance. CONCLUSIONS: Evaluation of the expression of genes for resistance to antineoplastic drugs in childhood acute lymphoblastic leukemia upon diagnosis, and particularly the expression of the LRP gene, may be of clinical relevance, and should be the object of prospective studies. SN - 1516-3180 UR - https://www.unboundmedicine.com/medline/citation/15543372/Multiple_drug_resistance_protein__MDR_1__multidrug_resistance_related_protein__MRP__and_lung_resistance_protein__LRP__gene_expression_in_childhood_acute_lymphoblastic_leukemia_ L2 - https://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802004000400007&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -