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Enatiomeric analysis of simendan by CE with beta-CD as chiral selector compared with CMPA-HPLC.
Biomed Chromatogr. 2004 Dec; 18(10):866-71.BC

Abstract

The chiral separation of simendan enantiomers using capillary electrophoresis was studied with beta-cyclodextrin (beta-CD) as chiral selector. The influences of the concentration and pH of borate buffer solution, beta-CD concentration and methanol content in the background electrolyte were investigated. These factors were compared with those in an HPLC with beta-CD as chiral mobile phase additive (CMPA-HPLC). The quantification properties of the developed CE method were examined. A baseline separation of simendan enantiomers was achieved in the background electrolyte of 20 mmol/L borate buffer (pH 11.0) containing 12 mmol/L beta-CD-methanol (50:50 in volume ratio). The CE method is comparable with CMPA-HPLC in chiral resolution, although the optimal pH in CE (11.0) is much higher than that (6.0) in CMPA-HPLC. This chiral CE method is applicable to the quantitative ananlysis and enantiomeric excess value determination of L-simendan.

Authors+Show Affiliations

Li F
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. fameili@hotmail.com
Zhang D
No affiliation info available
Lu X
No affiliation info available
Wang Y
No affiliation info available
Xiong Z
No affiliation info available

MeSH

BoratesBuffersCardiotonic AgentsChromatography, High Pressure LiquidElectrophoresis, CapillaryHydrazonesHydrogen-Ion ConcentrationIndicators and ReagentsPyridazinesSimendanStereoisomerismbeta-Cyclodextrins

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15543646

Citation

Li, Famei, et al. "Enatiomeric Analysis of Simendan By CE With beta-CD as Chiral Selector Compared With CMPA-HPLC." Biomedical Chromatography : BMC, vol. 18, no. 10, 2004, pp. 866-71.
Li F, Zhang D, Lu X, et al. Enatiomeric analysis of simendan by CE with beta-CD as chiral selector compared with CMPA-HPLC. Biomed Chromatogr. 2004;18(10):866-71.
Li, F., Zhang, D., Lu, X., Wang, Y., & Xiong, Z. (2004). Enatiomeric analysis of simendan by CE with beta-CD as chiral selector compared with CMPA-HPLC. Biomedical Chromatography : BMC, 18(10), 866-71.
Li F, et al. Enatiomeric Analysis of Simendan By CE With beta-CD as Chiral Selector Compared With CMPA-HPLC. Biomed Chromatogr. 2004;18(10):866-71. PubMed PMID: 15543646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enatiomeric analysis of simendan by CE with beta-CD as chiral selector compared with CMPA-HPLC. AU - Li,Famei, AU - Zhang,Dandan, AU - Lu,Xiumei, AU - Wang,Yuling, AU - Xiong,Zhili, PY - 2004/11/16/pubmed PY - 2005/5/6/medline PY - 2004/11/16/entrez SP - 866 EP - 71 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 18 IS - 10 N2 - The chiral separation of simendan enantiomers using capillary electrophoresis was studied with beta-cyclodextrin (beta-CD) as chiral selector. The influences of the concentration and pH of borate buffer solution, beta-CD concentration and methanol content in the background electrolyte were investigated. These factors were compared with those in an HPLC with beta-CD as chiral mobile phase additive (CMPA-HPLC). The quantification properties of the developed CE method were examined. A baseline separation of simendan enantiomers was achieved in the background electrolyte of 20 mmol/L borate buffer (pH 11.0) containing 12 mmol/L beta-CD-methanol (50:50 in volume ratio). The CE method is comparable with CMPA-HPLC in chiral resolution, although the optimal pH in CE (11.0) is much higher than that (6.0) in CMPA-HPLC. This chiral CE method is applicable to the quantitative ananlysis and enantiomeric excess value determination of L-simendan. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/15543646/Enatiomeric_analysis_of_simendan_by_CE_with_beta_CD_as_chiral_selector_compared_with_CMPA_HPLC_ DB - PRIME DP - Unbound Medicine ER -