Tags

Type your tag names separated by a space and hit enter

Restoration of CD4 T-cell responses to cytomegalovirus is short-lived in severely immunodeficient HIV-infected patients responding to highly active antiretroviral therapy.
HIV Med. 2004 Nov; 5(6):407-14.HM

Abstract

OBJECTIVES

To define the level of pathogen-specific immune reconstitution persisting over 3 to 5 years of highly active antiretroviral therapy (HAART) in HIV-infected patients who began therapy with CD4 T-cell counts below 50 cells/microL.

METHODS

Cytomegalovirus (CMV)-specific T-cell responses were analysed in adult HIV-1-infected patients with nadir CD4 T-cell counts below 50 cells/microL before HAART. CMV-specific CD4 T-cell responses were measured by interferon-gamma enzyme-linked immunospot assay (ELISpot assay), lymphoproliferation and interferon-gamma levels in cell culture supernatants.

RESULTS

CD4 T-cell responses to CMV were low in untreated patients and remained low during the first year on HAART, but increased progressively to levels similar to those found in HIV-seronegative CMV-seropositive controls at 3 years. Responses then declined markedly and at 5 years were lower than controls. This could not be explained by changes in CD4 or CD8 T-cell counts or plasma HIV RNA levels. Interferon-gamma and interleukin-5 responses to a mitogen were maintained or elevated.

CONCLUSIONS

CMV-specific CD4 T-cell responses were found to decline after 3-5 years on HAART and may provide inadequate long-term protection against CMV disease in patients who are severely immunodeficient prior to treatment.

Authors+Show Affiliations

Department of Clinical Immunology and Biochemical Genetics, Royal Perth Hospital, Perth, WA, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15544692

Citation

Keane, N M., et al. "Restoration of CD4 T-cell Responses to Cytomegalovirus Is Short-lived in Severely Immunodeficient HIV-infected Patients Responding to Highly Active Antiretroviral Therapy." HIV Medicine, vol. 5, no. 6, 2004, pp. 407-14.
Keane NM, Price P, Lee S, et al. Restoration of CD4 T-cell responses to cytomegalovirus is short-lived in severely immunodeficient HIV-infected patients responding to highly active antiretroviral therapy. HIV Med. 2004;5(6):407-14.
Keane, N. M., Price, P., Lee, S., Almeida, C. A., Stone, S. F., James, I., & French, M. A. (2004). Restoration of CD4 T-cell responses to cytomegalovirus is short-lived in severely immunodeficient HIV-infected patients responding to highly active antiretroviral therapy. HIV Medicine, 5(6), 407-14.
Keane NM, et al. Restoration of CD4 T-cell Responses to Cytomegalovirus Is Short-lived in Severely Immunodeficient HIV-infected Patients Responding to Highly Active Antiretroviral Therapy. HIV Med. 2004;5(6):407-14. PubMed PMID: 15544692.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Restoration of CD4 T-cell responses to cytomegalovirus is short-lived in severely immunodeficient HIV-infected patients responding to highly active antiretroviral therapy. AU - Keane,N M, AU - Price,P, AU - Lee,S, AU - Almeida,C A, AU - Stone,S F, AU - James,I, AU - French,M A, PY - 2004/11/17/pubmed PY - 2005/2/17/medline PY - 2004/11/17/entrez SP - 407 EP - 14 JF - HIV medicine JO - HIV Med VL - 5 IS - 6 N2 - OBJECTIVES: To define the level of pathogen-specific immune reconstitution persisting over 3 to 5 years of highly active antiretroviral therapy (HAART) in HIV-infected patients who began therapy with CD4 T-cell counts below 50 cells/microL. METHODS: Cytomegalovirus (CMV)-specific T-cell responses were analysed in adult HIV-1-infected patients with nadir CD4 T-cell counts below 50 cells/microL before HAART. CMV-specific CD4 T-cell responses were measured by interferon-gamma enzyme-linked immunospot assay (ELISpot assay), lymphoproliferation and interferon-gamma levels in cell culture supernatants. RESULTS: CD4 T-cell responses to CMV were low in untreated patients and remained low during the first year on HAART, but increased progressively to levels similar to those found in HIV-seronegative CMV-seropositive controls at 3 years. Responses then declined markedly and at 5 years were lower than controls. This could not be explained by changes in CD4 or CD8 T-cell counts or plasma HIV RNA levels. Interferon-gamma and interleukin-5 responses to a mitogen were maintained or elevated. CONCLUSIONS: CMV-specific CD4 T-cell responses were found to decline after 3-5 years on HAART and may provide inadequate long-term protection against CMV disease in patients who are severely immunodeficient prior to treatment. SN - 1464-2662 UR - https://www.unboundmedicine.com/medline/citation/15544692/Restoration_of_CD4_T_cell_responses_to_cytomegalovirus_is_short_lived_in_severely_immunodeficient_HIV_infected_patients_responding_to_highly_active_antiretroviral_therapy_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1464-2662&date=2004&volume=5&issue=6&spage=407 DB - PRIME DP - Unbound Medicine ER -