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The role of NOS2 and NOS3 in renal protein and arginine metabolism during early endotoxemia in mice.
Am J Physiol Renal Physiol 2005; 288(4):F816-22AJ

Abstract

Previously, we observed an enhanced renal protein synthesis and increased de novo arginine production in the early response to endotoxemia in wild-type Swiss mice (Hallemeesch MM, Soeters PB, and Deutz NE. Am J Physiol Renal Physiol 282: F316-F323, 2002). To establish whether these changes are regulated by nitric oxide (NO) synthesized by NO synthase isoforms NOS2 and NOS3, we studied C57BL6/J wild-type (WT), NOS2-deficient (NOS2(-/-)), and NOS3-deficient (NOS3(-/-)) mice under baseline (unstimulated) and LPS-treated conditions. The metabolism of renal protein, amino acid, and arginine was studied at the whole body level and across the kidney by infusing the stable isotopes l-[phenyl-(2)H(5)]phenylalanine, l-[phenyl-(2)H(2)]tyrosine, l-guanidino-[(15)N(2)]arginine, and l-[ureido-(13)C,(2)H(2)]citrulline. Renal blood flow was measured using radioactive PAH extraction. Under baseline conditions, renal blood flow was significantly reduced in NOS2(-/-) mice (0.29 +/- 0.01 vs. 0.48 +/- 0.07 ml.10 g body wt(-1).min(-1) in WT) (P < 0.05), and de novo arginine production was lower in NOS2(-/-) mice. After LPS challenge, renal protein turnover and arginine production increased in all three groups (P < 0.05), even though renal de novo arginine synthesis did not increase. The expected increase in renal citrulline production and disposal after LPS was not observed in NOS2(-/-) mice (P = 0.06). Collectively, these data show that NOS2 is constitutively expressed in the kidney and remarkably functional as it affects renal blood flow and de novo arginine production under baseline conditions and is important for the increase in renal citrulline turnover during endotoxemia. NOS3, in contrast, appears less important for renal metabolism. The increase in renal protein turnover during endotoxemia does not depend on NOS2 or NOS3 activity.

Authors+Show Affiliations

Dept. of Surgery, Maastricht Univ., PO Box 616, NL-6200 MD Maastricht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15547116

Citation

Luiking, Yvette C., et al. "The Role of NOS2 and NOS3 in Renal Protein and Arginine Metabolism During Early Endotoxemia in Mice." American Journal of Physiology. Renal Physiology, vol. 288, no. 4, 2005, pp. F816-22.
Luiking YC, Hallemeesch MM, Lamers WH, et al. The role of NOS2 and NOS3 in renal protein and arginine metabolism during early endotoxemia in mice. Am J Physiol Renal Physiol. 2005;288(4):F816-22.
Luiking, Y. C., Hallemeesch, M. M., Lamers, W. H., & Deutz, N. E. (2005). The role of NOS2 and NOS3 in renal protein and arginine metabolism during early endotoxemia in mice. American Journal of Physiology. Renal Physiology, 288(4), pp. F816-22.
Luiking YC, et al. The Role of NOS2 and NOS3 in Renal Protein and Arginine Metabolism During Early Endotoxemia in Mice. Am J Physiol Renal Physiol. 2005;288(4):F816-22. PubMed PMID: 15547116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of NOS2 and NOS3 in renal protein and arginine metabolism during early endotoxemia in mice. AU - Luiking,Yvette C, AU - Hallemeesch,Marcella M, AU - Lamers,Wouter H, AU - Deutz,Nicolaas E P, Y1 - 2004/11/16/ PY - 2004/11/18/pubmed PY - 2005/4/19/medline PY - 2004/11/18/entrez SP - F816 EP - 22 JF - American journal of physiology. Renal physiology JO - Am. J. Physiol. Renal Physiol. VL - 288 IS - 4 N2 - Previously, we observed an enhanced renal protein synthesis and increased de novo arginine production in the early response to endotoxemia in wild-type Swiss mice (Hallemeesch MM, Soeters PB, and Deutz NE. Am J Physiol Renal Physiol 282: F316-F323, 2002). To establish whether these changes are regulated by nitric oxide (NO) synthesized by NO synthase isoforms NOS2 and NOS3, we studied C57BL6/J wild-type (WT), NOS2-deficient (NOS2(-/-)), and NOS3-deficient (NOS3(-/-)) mice under baseline (unstimulated) and LPS-treated conditions. The metabolism of renal protein, amino acid, and arginine was studied at the whole body level and across the kidney by infusing the stable isotopes l-[phenyl-(2)H(5)]phenylalanine, l-[phenyl-(2)H(2)]tyrosine, l-guanidino-[(15)N(2)]arginine, and l-[ureido-(13)C,(2)H(2)]citrulline. Renal blood flow was measured using radioactive PAH extraction. Under baseline conditions, renal blood flow was significantly reduced in NOS2(-/-) mice (0.29 +/- 0.01 vs. 0.48 +/- 0.07 ml.10 g body wt(-1).min(-1) in WT) (P < 0.05), and de novo arginine production was lower in NOS2(-/-) mice. After LPS challenge, renal protein turnover and arginine production increased in all three groups (P < 0.05), even though renal de novo arginine synthesis did not increase. The expected increase in renal citrulline production and disposal after LPS was not observed in NOS2(-/-) mice (P = 0.06). Collectively, these data show that NOS2 is constitutively expressed in the kidney and remarkably functional as it affects renal blood flow and de novo arginine production under baseline conditions and is important for the increase in renal citrulline turnover during endotoxemia. NOS3, in contrast, appears less important for renal metabolism. The increase in renal protein turnover during endotoxemia does not depend on NOS2 or NOS3 activity. SN - 1931-857X UR - https://www.unboundmedicine.com/medline/citation/15547116/The_role_of_NOS2_and_NOS3_in_renal_protein_and_arginine_metabolism_during_early_endotoxemia_in_mice_ L2 - http://www.physiology.org/doi/full/10.1152/ajprenal.00308.2004?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -