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Demonstration of human telomerase reverse transcriptase by in situ hybridization in lung carcinoma.
Oncol Rep. 2004 Dec; 12(6):1227-32.OR

Abstract

Telomerase activity is present in most malignant tumors and provides a mechanism for unlimited replication of neoplastic cells. This study was undertaken to investigate the expression of the gene encoding human telomerase reverse transcriptase (hTERT), the telomerase catalytic subunit gene in lung carcinoma, by in situ hybridization (ISH). hTERT is associated with telomerase activity, and overexpressed in most lung carcinomas. We assayed hTERT gene expression by ISH to study telomerase activity in lung carcinomas of 27 patients, and compared these results with those of telomerase activity by telomeric repeat amplification protocol (TRAP) assay. TERT gene expression by ISH was detected mainly in the nuclei of lung carcinoma cells. Some specimens showed a significant expression in only infiltrating lymphocytes. hTERT gene expression by ISH was found in 16 of 27 (59%) cases. On the other hand, telomerase activity by TRAP assay was detected in 21 of 27 (78%) cases. In 7 cases, TRAP assay detected telomerase activity, but ISH did not detect hTERT expression. TRAP assay might detect telomerase activity in not only carcinoma cells, but also in infiltrating lymphocytes. Our findings therefore suggest that ISH-based analysis of hTERT gene expression is superior to TRAP assay as a means of determining telomerase status during carcinogenesis.

Authors+Show Affiliations

Department of Clinical Pathology, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan. mitchy@nms.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15547742

Citation

Fukushima, Mitsuhiro, et al. "Demonstration of Human Telomerase Reverse Transcriptase By in Situ Hybridization in Lung Carcinoma." Oncology Reports, vol. 12, no. 6, 2004, pp. 1227-32.
Fukushima M, Shimomura N, Nakamura K, et al. Demonstration of human telomerase reverse transcriptase by in situ hybridization in lung carcinoma. Oncol Rep. 2004;12(6):1227-32.
Fukushima, M., Shimomura, N., Nakamura, K., Kammori, M., Koizumi, K., Shimizu, K., & Takubo, K. (2004). Demonstration of human telomerase reverse transcriptase by in situ hybridization in lung carcinoma. Oncology Reports, 12(6), 1227-32.
Fukushima M, et al. Demonstration of Human Telomerase Reverse Transcriptase By in Situ Hybridization in Lung Carcinoma. Oncol Rep. 2004;12(6):1227-32. PubMed PMID: 15547742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Demonstration of human telomerase reverse transcriptase by in situ hybridization in lung carcinoma. AU - Fukushima,Mitsuhiro, AU - Shimomura,Naotaka, AU - Nakamura,Ken-Ichi, AU - Kammori,Makoto, AU - Koizumi,Kiyoshi, AU - Shimizu,Kazuo, AU - Takubo,Kaiyo, PY - 2004/11/18/pubmed PY - 2005/3/30/medline PY - 2004/11/18/entrez SP - 1227 EP - 32 JF - Oncology reports JO - Oncol Rep VL - 12 IS - 6 N2 - Telomerase activity is present in most malignant tumors and provides a mechanism for unlimited replication of neoplastic cells. This study was undertaken to investigate the expression of the gene encoding human telomerase reverse transcriptase (hTERT), the telomerase catalytic subunit gene in lung carcinoma, by in situ hybridization (ISH). hTERT is associated with telomerase activity, and overexpressed in most lung carcinomas. We assayed hTERT gene expression by ISH to study telomerase activity in lung carcinomas of 27 patients, and compared these results with those of telomerase activity by telomeric repeat amplification protocol (TRAP) assay. TERT gene expression by ISH was detected mainly in the nuclei of lung carcinoma cells. Some specimens showed a significant expression in only infiltrating lymphocytes. hTERT gene expression by ISH was found in 16 of 27 (59%) cases. On the other hand, telomerase activity by TRAP assay was detected in 21 of 27 (78%) cases. In 7 cases, TRAP assay detected telomerase activity, but ISH did not detect hTERT expression. TRAP assay might detect telomerase activity in not only carcinoma cells, but also in infiltrating lymphocytes. Our findings therefore suggest that ISH-based analysis of hTERT gene expression is superior to TRAP assay as a means of determining telomerase status during carcinogenesis. SN - 1021-335X UR - https://www.unboundmedicine.com/medline/citation/15547742/Demonstration_of_human_telomerase_reverse_transcriptase_by_in_situ_hybridization_in_lung_carcinoma_ L2 - http://www.spandidos-publications.com/or/12/6/1227 DB - PRIME DP - Unbound Medicine ER -