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Human brain endothelium: coexpression and function of vanilloid and endocannabinoid receptors.
Brain Res Mol Brain Res 2004; 132(1):87-92BR

Abstract

The arachidonic acid derivative, 2-arachidonoyl-glycerol (2-AG), was initially isolated from gut and brain; it is also produced and released from blood and vascular cells. Many of the 2-AG-induced cellular responses (i.e., neuromodulation, cytoprotection and vasodilation) are mediated by cannabinoid receptors CB1 and CB2. The findings presented here demonstrate the expression of CB1, CB2 and TRPV1 receptors on cerebromicrovascular endothelial cells (HBEC). The expression of TRPV1, CB1 and CB2 receptor mRNA and proteins were demonstrated by RT-PCR and polyclonal antibodies, respectively. The endocannabinoid 2-AG, and other related compounds [anandamide (ANA), methanandamide (m-ANA), N-(4-hydroxyphenyl-arachidonyl-ethanolamide) (AM404) and capsaicin] dose-dependently stimulated Ca2+ influx in HBEC. The selective TRPV1 receptor antagonist (capsazepine), CB1 receptor antagonist (SR141716A) and CB2 receptor antagonist (SR144528) inhibited these responses. The effects of capsaicin, a specific agonist for TRPV1 receptors, were inhibited by capsazepine, but only weakly by CB1 or CB2 receptor antagonists. 2-AG also induced phosphorylation of vasodilator-stimulated phosphoprotein (VASP); this response was mediated by VR1 receptors. These studies clearly indicate that 2-AG and other related compounds may function as agonists on VR1 receptors, as well as CB1 and CB2 receptors, and implicated these factors in various HBEC functions.

Authors+Show Affiliations

Resuscitative Medicine Department, Naval Medical Research Center, Bethesda, MD, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15548432

Citation

Golech, Susanne Andrea, et al. "Human Brain Endothelium: Coexpression and Function of Vanilloid and Endocannabinoid Receptors." Brain Research. Molecular Brain Research, vol. 132, no. 1, 2004, pp. 87-92.
Golech SA, McCarron RM, Chen Y, et al. Human brain endothelium: coexpression and function of vanilloid and endocannabinoid receptors. Brain Res Mol Brain Res. 2004;132(1):87-92.
Golech, S. A., McCarron, R. M., Chen, Y., Bembry, J., Lenz, F., Mechoulam, R., ... Spatz, M. (2004). Human brain endothelium: coexpression and function of vanilloid and endocannabinoid receptors. Brain Research. Molecular Brain Research, 132(1), pp. 87-92.
Golech SA, et al. Human Brain Endothelium: Coexpression and Function of Vanilloid and Endocannabinoid Receptors. Brain Res Mol Brain Res. 2004 Dec 6;132(1):87-92. PubMed PMID: 15548432.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human brain endothelium: coexpression and function of vanilloid and endocannabinoid receptors. AU - Golech,Susanne Andrea, AU - McCarron,Richard M, AU - Chen,Ye, AU - Bembry,Joliet, AU - Lenz,Frederick, AU - Mechoulam,Raphael, AU - Shohami,Esther, AU - Spatz,Maria, PY - 2004/08/28/accepted PY - 2004/11/19/pubmed PY - 2005/3/3/medline PY - 2004/11/19/entrez SP - 87 EP - 92 JF - Brain research. Molecular brain research JO - Brain Res. Mol. Brain Res. VL - 132 IS - 1 N2 - The arachidonic acid derivative, 2-arachidonoyl-glycerol (2-AG), was initially isolated from gut and brain; it is also produced and released from blood and vascular cells. Many of the 2-AG-induced cellular responses (i.e., neuromodulation, cytoprotection and vasodilation) are mediated by cannabinoid receptors CB1 and CB2. The findings presented here demonstrate the expression of CB1, CB2 and TRPV1 receptors on cerebromicrovascular endothelial cells (HBEC). The expression of TRPV1, CB1 and CB2 receptor mRNA and proteins were demonstrated by RT-PCR and polyclonal antibodies, respectively. The endocannabinoid 2-AG, and other related compounds [anandamide (ANA), methanandamide (m-ANA), N-(4-hydroxyphenyl-arachidonyl-ethanolamide) (AM404) and capsaicin] dose-dependently stimulated Ca2+ influx in HBEC. The selective TRPV1 receptor antagonist (capsazepine), CB1 receptor antagonist (SR141716A) and CB2 receptor antagonist (SR144528) inhibited these responses. The effects of capsaicin, a specific agonist for TRPV1 receptors, were inhibited by capsazepine, but only weakly by CB1 or CB2 receptor antagonists. 2-AG also induced phosphorylation of vasodilator-stimulated phosphoprotein (VASP); this response was mediated by VR1 receptors. These studies clearly indicate that 2-AG and other related compounds may function as agonists on VR1 receptors, as well as CB1 and CB2 receptors, and implicated these factors in various HBEC functions. SN - 0169-328X UR - https://www.unboundmedicine.com/medline/citation/15548432/Human_brain_endothelium:_coexpression_and_function_of_vanilloid_and_endocannabinoid_receptors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0169-328X(04)00402-4 DB - PRIME DP - Unbound Medicine ER -