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Changes in P2X3 purinoceptors in sensory ganglia of the mouse during embryonic and postnatal development.
Histochem Cell Biol 2004; 122(6):539-51HC

Abstract

The expression of the P2X(3) nucleotide receptor in embryonic day 14-18, postnatal day 1-14 and adult mouse sensory ganglia was examined using immunohistochemistry. Nearly all sensory neurons in dorsal root ganglia, trigeminal ganglia and nodose ganglia in embryos at embryonic day 14 expressed P2X(3) receptors, but after birth there was a gradual decline to about 50% of neurons showing positive immunostaining for P2X(3). In embryos there were only small neurons, while from postnatal day 7 both large and small neurons were present. Isolectin B(4) (IB(4))-positive neurons in dorsal, trigeminal and nodose ganglia did not appear until birth, but the numbers increased to about 50% by postnatal day 14 when a high proportion of IB(4)-positive neurons were also positively labelled for the P2X(3) receptor. About 10% of neurons in dorsal, trigeminal and nodose ganglia were positive for calcitonin gene-related peptide in embryos, nearly all of which stained for P2X(3) receptors. This increased postnatally to about 35-40% in adults, although only a few colocalised with P2X(3) receptors. Neurofilament 200 was expressed in about 50% of neurons in trigeminal ganglia in the embryo, and this level persisted postnatally. All neurofilament 200-positive neurons stained for P2X(3) in embryonic dorsal root ganglia, trigeminal ganglia and nodose ganglia, but by adulthood this was significantly reduced. The neurons that were positive for calbindin in embryonic dorsal, trigeminal and nodose ganglia showed colocalisation with P2X(3) receptors, but few showed colocalisation postnatally.

Authors+Show Affiliations

Autonomic Neuroscience Institute, Royal Free & University College Medical School, Rowland Hill Street, London, NW3 2PF, UK.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15549366

Citation

Ruan, Huai Zhen, et al. "Changes in P2X3 Purinoceptors in Sensory Ganglia of the Mouse During Embryonic and Postnatal Development." Histochemistry and Cell Biology, vol. 122, no. 6, 2004, pp. 539-51.
Ruan HZ, Moules E, Burnstock G. Changes in P2X3 purinoceptors in sensory ganglia of the mouse during embryonic and postnatal development. Histochem Cell Biol. 2004;122(6):539-51.
Ruan, H. Z., Moules, E., & Burnstock, G. (2004). Changes in P2X3 purinoceptors in sensory ganglia of the mouse during embryonic and postnatal development. Histochemistry and Cell Biology, 122(6), pp. 539-51.
Ruan HZ, Moules E, Burnstock G. Changes in P2X3 Purinoceptors in Sensory Ganglia of the Mouse During Embryonic and Postnatal Development. Histochem Cell Biol. 2004;122(6):539-51. PubMed PMID: 15549366.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in P2X3 purinoceptors in sensory ganglia of the mouse during embryonic and postnatal development. AU - Ruan,Huai Zhen, AU - Moules,Eamonn, AU - Burnstock,Geoffrey, Y1 - 2004/11/12/ PY - 2004/09/21/accepted PY - 2004/11/19/pubmed PY - 2005/6/1/medline PY - 2004/11/19/entrez SP - 539 EP - 51 JF - Histochemistry and cell biology JO - Histochem. Cell Biol. VL - 122 IS - 6 N2 - The expression of the P2X(3) nucleotide receptor in embryonic day 14-18, postnatal day 1-14 and adult mouse sensory ganglia was examined using immunohistochemistry. Nearly all sensory neurons in dorsal root ganglia, trigeminal ganglia and nodose ganglia in embryos at embryonic day 14 expressed P2X(3) receptors, but after birth there was a gradual decline to about 50% of neurons showing positive immunostaining for P2X(3). In embryos there were only small neurons, while from postnatal day 7 both large and small neurons were present. Isolectin B(4) (IB(4))-positive neurons in dorsal, trigeminal and nodose ganglia did not appear until birth, but the numbers increased to about 50% by postnatal day 14 when a high proportion of IB(4)-positive neurons were also positively labelled for the P2X(3) receptor. About 10% of neurons in dorsal, trigeminal and nodose ganglia were positive for calcitonin gene-related peptide in embryos, nearly all of which stained for P2X(3) receptors. This increased postnatally to about 35-40% in adults, although only a few colocalised with P2X(3) receptors. Neurofilament 200 was expressed in about 50% of neurons in trigeminal ganglia in the embryo, and this level persisted postnatally. All neurofilament 200-positive neurons stained for P2X(3) in embryonic dorsal root ganglia, trigeminal ganglia and nodose ganglia, but by adulthood this was significantly reduced. The neurons that were positive for calbindin in embryonic dorsal, trigeminal and nodose ganglia showed colocalisation with P2X(3) receptors, but few showed colocalisation postnatally. SN - 0948-6143 UR - https://www.unboundmedicine.com/medline/citation/15549366/Changes_in_P2X3_purinoceptors_in_sensory_ganglia_of_the_mouse_during_embryonic_and_postnatal_development_ L2 - https://dx.doi.org/10.1007/s00418-004-0714-9 DB - PRIME DP - Unbound Medicine ER -