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Scoparia dulcis, a traditional antidiabetic plant, protects against streptozotocin induced oxidative stress and apoptosis in vitro and in vivo.

Abstract

Oxidative stress is implicated in the pathogenesis of diabetic complications. The experiments were performed on normal and experimental male Wistar rats treated with Scoparia dulcis plant extract (SPEt). The effect of SPEt was tested on streptozotocin (STZ) treated Rat insulinoma cell lines (RINm5F cells) and isolated islets in vitro. Administration of an aqueous extract of Scoparia dulcis by intragastric intubation (po) at a dose of 200 mg/kg body weight significantly decreased the blood glucose and lipid peroxidative marker thiobarbituric acid reactive substances (TBARS) with significant increase in the activities of plasma insulin, pancreatic superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) in streptozotocin diabetic rats at the end of 15 days treatment. Streptozotocin at a dose of 10 mug/mL evoked 6-fold stimulation of insulin secretion from isolated islets indicating its insulin secretagogue activity. The extract markedly reduced the STZ-induced lipidperoxidation in RINm5F cells. Further, SPEt protected STZ-mediated cytotoxicity and nitric oxide (NO) production in RINm5F cells. Treatment of RINm5F cells with 5 mM STZ and 10 mug of SPEt completely abrogated apoptosis induced by STZ, suggesting the involvement of oxidative stress. Flow cytometric assessment on the level of intracellular peroxides using fluorescent probe 2'7'-dichlorofluorescein diacetate (DCF-DA) confirmed that STZ (46%) induced an intracellular oxidative stress in RINm5F cells, which was suppressed by SPEt (21%). In addition, SPEt also reduced (33%) the STZ-induced apoptosis (72%) in RINm5F cells indicating the mode of protection of SPEt on RIN m5Fcells, islets, and pancreatic beta-cell mass (histopathological observations). Present study thus confirms antihyperglycemic effect of SPEt and also demonstrated the consistently strong antioxidant properties of Scoparia dulcis used in the traditional medicine.

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  • Authors+Show Affiliations

    ,

    Department of Biochemistry, Faculty of Science, Annamalai University, Annamalainagar 608 002, Tamil Nadu, India.

    , ,

    Source

    MeSH

    Animals
    Apoptosis
    Blood Glucose
    Catalase
    Cell Line, Tumor
    Diabetes Mellitus, Experimental
    Insulin
    Insulinoma
    Islets of Langerhans
    Lipid Peroxidation
    Male
    Oxidative Stress
    Pancreatic Neoplasms
    Phytotherapy
    Plant Extracts
    Rats
    Rats, Wistar
    Scoparia
    Streptozocin
    Superoxide Dismutase
    Thiobarbituric Acid Reactive Substances

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    15549711

    Citation

    Latha, Muniappan, et al. "Scoparia Dulcis, a Traditional Antidiabetic Plant, Protects Against Streptozotocin Induced Oxidative Stress and Apoptosis in Vitro and in Vivo." Journal of Biochemical and Molecular Toxicology, vol. 18, no. 5, 2004, pp. 261-72.
    Latha M, Pari L, Sitasawad S, et al. Scoparia dulcis, a traditional antidiabetic plant, protects against streptozotocin induced oxidative stress and apoptosis in vitro and in vivo. J Biochem Mol Toxicol. 2004;18(5):261-72.
    Latha, M., Pari, L., Sitasawad, S., & Bhonde, R. (2004). Scoparia dulcis, a traditional antidiabetic plant, protects against streptozotocin induced oxidative stress and apoptosis in vitro and in vivo. Journal of Biochemical and Molecular Toxicology, 18(5), pp. 261-72.
    Latha M, et al. Scoparia Dulcis, a Traditional Antidiabetic Plant, Protects Against Streptozotocin Induced Oxidative Stress and Apoptosis in Vitro and in Vivo. J Biochem Mol Toxicol. 2004;18(5):261-72. PubMed PMID: 15549711.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Scoparia dulcis, a traditional antidiabetic plant, protects against streptozotocin induced oxidative stress and apoptosis in vitro and in vivo. AU - Latha,Muniappan, AU - Pari,Leelavinothan, AU - Sitasawad,Sandhya, AU - Bhonde,Ramesh, PY - 2004/11/19/pubmed PY - 2005/5/25/medline PY - 2004/11/19/entrez SP - 261 EP - 72 JF - Journal of biochemical and molecular toxicology JO - J. Biochem. Mol. Toxicol. VL - 18 IS - 5 N2 - Oxidative stress is implicated in the pathogenesis of diabetic complications. The experiments were performed on normal and experimental male Wistar rats treated with Scoparia dulcis plant extract (SPEt). The effect of SPEt was tested on streptozotocin (STZ) treated Rat insulinoma cell lines (RINm5F cells) and isolated islets in vitro. Administration of an aqueous extract of Scoparia dulcis by intragastric intubation (po) at a dose of 200 mg/kg body weight significantly decreased the blood glucose and lipid peroxidative marker thiobarbituric acid reactive substances (TBARS) with significant increase in the activities of plasma insulin, pancreatic superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) in streptozotocin diabetic rats at the end of 15 days treatment. Streptozotocin at a dose of 10 mug/mL evoked 6-fold stimulation of insulin secretion from isolated islets indicating its insulin secretagogue activity. The extract markedly reduced the STZ-induced lipidperoxidation in RINm5F cells. Further, SPEt protected STZ-mediated cytotoxicity and nitric oxide (NO) production in RINm5F cells. Treatment of RINm5F cells with 5 mM STZ and 10 mug of SPEt completely abrogated apoptosis induced by STZ, suggesting the involvement of oxidative stress. Flow cytometric assessment on the level of intracellular peroxides using fluorescent probe 2'7'-dichlorofluorescein diacetate (DCF-DA) confirmed that STZ (46%) induced an intracellular oxidative stress in RINm5F cells, which was suppressed by SPEt (21%). In addition, SPEt also reduced (33%) the STZ-induced apoptosis (72%) in RINm5F cells indicating the mode of protection of SPEt on RIN m5Fcells, islets, and pancreatic beta-cell mass (histopathological observations). Present study thus confirms antihyperglycemic effect of SPEt and also demonstrated the consistently strong antioxidant properties of Scoparia dulcis used in the traditional medicine. SN - 1095-6670 UR - https://www.unboundmedicine.com/medline/citation/15549711/Scoparia_dulcis_a_traditional_antidiabetic_plant_protects_against_streptozotocin_induced_oxidative_stress_and_apoptosis_in_vitro_and_in_vivo_ L2 - https://doi.org/10.1002/jbt.20035 DB - PRIME DP - Unbound Medicine ER -