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Cytokine profile of sickle cell disease in Oman.
Am J Hematol. 2004 Dec; 77(4):323-8.AJ

Abstract

The aim of our study was to assess the cytokine profile of sickle cell disease (SCD) patients in steady state and in vaso-occlusive crisis (VOC). VOC has a complex nature, involving interactions between sickle red blood cells (RBC), the endothelium, and leucocytes. Endothelial damage due to recurrent adhesion of sickle RBCs may disrupt endothelial function, leading to altered cytokine release. It is therefore pertinent to study the cytokine profile of SCD patients in steady state and in crisis prior to exploring its contribution to vaso-occlusive manifestations, since it is believed that an altered balance of proinflammatory and anti-inflammatory cytokines plays an important role in painful crisis. Cytokines including IL-1beta, IL-2, IL-4, IL-6, IL-8, TNF-alpha, and IFN-gamma were measured by commercially available ELISA kits in SCD patients (n = 60); in steady state (n = 26) and in painful crisis (n = 34) and compared with nonanemic age- and sex-matched normal Omani controls (n = 20). SCD patients in crisis showed elevated levels of TNF-alpha (P < 0.092) and IL-6 (P < 0.024) when compared with steady state. It was also observed that SCD patients in steady state showed a significant elevation in IL-1beta (P < 0.04), IL-6 (P < 0.0001), and IFN-gamma (P < 0.02) as compared to normal subjects. It is thus evident that both type I and type II cytokines are significantly altered in SCD patients. In steady state, type II proinflammatory cytokines are elevated, whereas in crisis, an additional augmentation of type I cytokines occurs, with persistent elevation of type II cytokines, emphasizing the role of perturbed endothelium and activated monocytes in the pathophysiology of vaso-occlusion in sickle cell crisis.

Authors+Show Affiliations

Department of Haematology, Sultan Qaboos University Hospital, Sultanate of Oman. avp16@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15551290

Citation

Pathare, Anil, et al. "Cytokine Profile of Sickle Cell Disease in Oman." American Journal of Hematology, vol. 77, no. 4, 2004, pp. 323-8.
Pathare A, Al Kindi S, Alnaqdy AA, et al. Cytokine profile of sickle cell disease in Oman. Am J Hematol. 2004;77(4):323-8.
Pathare, A., Al Kindi, S., Alnaqdy, A. A., Daar, S., Knox-Macaulay, H., & Dennison, D. (2004). Cytokine profile of sickle cell disease in Oman. American Journal of Hematology, 77(4), 323-8.
Pathare A, et al. Cytokine Profile of Sickle Cell Disease in Oman. Am J Hematol. 2004;77(4):323-8. PubMed PMID: 15551290.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytokine profile of sickle cell disease in Oman. AU - Pathare,Anil, AU - Al Kindi,Salam, AU - Alnaqdy,Adel Alwan, AU - Daar,Shahina, AU - Knox-Macaulay,Huxley, AU - Dennison,David, PY - 2004/11/20/pubmed PY - 2005/1/19/medline PY - 2004/11/20/entrez SP - 323 EP - 8 JF - American journal of hematology JO - Am. J. Hematol. VL - 77 IS - 4 N2 - The aim of our study was to assess the cytokine profile of sickle cell disease (SCD) patients in steady state and in vaso-occlusive crisis (VOC). VOC has a complex nature, involving interactions between sickle red blood cells (RBC), the endothelium, and leucocytes. Endothelial damage due to recurrent adhesion of sickle RBCs may disrupt endothelial function, leading to altered cytokine release. It is therefore pertinent to study the cytokine profile of SCD patients in steady state and in crisis prior to exploring its contribution to vaso-occlusive manifestations, since it is believed that an altered balance of proinflammatory and anti-inflammatory cytokines plays an important role in painful crisis. Cytokines including IL-1beta, IL-2, IL-4, IL-6, IL-8, TNF-alpha, and IFN-gamma were measured by commercially available ELISA kits in SCD patients (n = 60); in steady state (n = 26) and in painful crisis (n = 34) and compared with nonanemic age- and sex-matched normal Omani controls (n = 20). SCD patients in crisis showed elevated levels of TNF-alpha (P < 0.092) and IL-6 (P < 0.024) when compared with steady state. It was also observed that SCD patients in steady state showed a significant elevation in IL-1beta (P < 0.04), IL-6 (P < 0.0001), and IFN-gamma (P < 0.02) as compared to normal subjects. It is thus evident that both type I and type II cytokines are significantly altered in SCD patients. In steady state, type II proinflammatory cytokines are elevated, whereas in crisis, an additional augmentation of type I cytokines occurs, with persistent elevation of type II cytokines, emphasizing the role of perturbed endothelium and activated monocytes in the pathophysiology of vaso-occlusion in sickle cell crisis. SN - 0361-8609 UR - https://www.unboundmedicine.com/medline/citation/15551290/Cytokine_profile_of_sickle_cell_disease_in_Oman_ L2 - https://doi.org/10.1002/ajh.20196 DB - PRIME DP - Unbound Medicine ER -