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[Amplification and typing of Sta56 gene of Orientia tsutsugamushi from Shandong province].
Zhonghua Liu Xing Bing Xue Za Zhi. 2004 Aug; 25(8):698-701.ZL

Abstract

OBJECTIVE

To clarify the gene type of Orientia tsutsugamushi (Ot) from Shandong province.

METHODS

Nested-polymerase chain reaction (nPCR) was used to identify the gene type of 23 isolated Ot strains, 2 pools of homogenized leptotrombidium (L.) scutellare, 10 blood specimens of scrub typhus patients, and at the same time to compare with the international reference strains Gilliam, Karp, Kato. Sequencing analysis of the Sta56 gene was also used to further identify the precise gene types.

RESULTS

Of the 35 samples, 33 had the same products in the amplification of template Ot-DNA. They all belonged to Kawasaki strains endemic in Japan while 2 (FXS4 and LHGM2 strain) belonged to Karp strains. The Sta56 gene sequence homologies to Japan Kawasaki strain of the 2 representative strains (B-16 and FXS2 strain) of the 33 samples were 94.22%, 95.21% respectively, but they were less than 75.87% to other prototype strains; The homologies to Karp strain of FXS4 and LHGM2 strain were 83.03%, 96.45% respectively. B-16 and FXS2 strain were designated as of types strain Japan Kawasaki, FXS4 and LHGM2 as Karp strain.

CONCLUSION

The results indicated that the dominant Ot strains in Shandong Province were similar to Kawasaki strains, but Karp strains also existed.

Authors+Show Affiliations

Liu YX
School of Public Health, Shandong University, Jinan 250012, China.
Gao Y
No affiliation info available
Zhao ZT
No affiliation info available
Zhang JL
No affiliation info available
Yang ZQ
No affiliation info available
Bu XP
No affiliation info available
Su JJ
No affiliation info available

MeSH

AnimalsDNA, BacterialGenotypeHumansMiceOrientia tsutsugamushiPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthScrub TyphusSequence HomologySerotyping

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15555396

Citation

Liu, Yun-Xi, et al. "[Amplification and Typing of Sta56 Gene of Orientia Tsutsugamushi From Shandong Province]." Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, vol. 25, no. 8, 2004, pp. 698-701.
Liu YX, Gao Y, Zhao ZT, et al. [Amplification and typing of Sta56 gene of Orientia tsutsugamushi from Shandong province]. Zhonghua Liu Xing Bing Xue Za Zhi. 2004;25(8):698-701.
Liu, Y. X., Gao, Y., Zhao, Z. T., Zhang, J. L., Yang, Z. Q., Bu, X. P., & Su, J. J. (2004). [Amplification and typing of Sta56 gene of Orientia tsutsugamushi from Shandong province]. Zhonghua Liu Xing Bing Xue Za Zhi = Zhonghua Liuxingbingxue Zazhi, 25(8), 698-701.
Liu YX, et al. [Amplification and Typing of Sta56 Gene of Orientia Tsutsugamushi From Shandong Province]. Zhonghua Liu Xing Bing Xue Za Zhi. 2004;25(8):698-701. PubMed PMID: 15555396.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Amplification and typing of Sta56 gene of Orientia tsutsugamushi from Shandong province]. AU - Liu,Yun-Xi, AU - Gao,Yuan, AU - Zhao,Zhong-Tang, AU - Zhang,Jing-Lan, AU - Yang,Zhan-Qing, AU - Bu,Xiu-Ping, AU - Su,Jing-Jing, PY - 2004/11/24/pubmed PY - 2005/6/15/medline PY - 2004/11/24/entrez SP - 698 EP - 701 JF - Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi JO - Zhonghua Liu Xing Bing Xue Za Zhi VL - 25 IS - 8 N2 - OBJECTIVE: To clarify the gene type of Orientia tsutsugamushi (Ot) from Shandong province. METHODS: Nested-polymerase chain reaction (nPCR) was used to identify the gene type of 23 isolated Ot strains, 2 pools of homogenized leptotrombidium (L.) scutellare, 10 blood specimens of scrub typhus patients, and at the same time to compare with the international reference strains Gilliam, Karp, Kato. Sequencing analysis of the Sta56 gene was also used to further identify the precise gene types. RESULTS: Of the 35 samples, 33 had the same products in the amplification of template Ot-DNA. They all belonged to Kawasaki strains endemic in Japan while 2 (FXS4 and LHGM2 strain) belonged to Karp strains. The Sta56 gene sequence homologies to Japan Kawasaki strain of the 2 representative strains (B-16 and FXS2 strain) of the 33 samples were 94.22%, 95.21% respectively, but they were less than 75.87% to other prototype strains; The homologies to Karp strain of FXS4 and LHGM2 strain were 83.03%, 96.45% respectively. B-16 and FXS2 strain were designated as of types strain Japan Kawasaki, FXS4 and LHGM2 as Karp strain. CONCLUSION: The results indicated that the dominant Ot strains in Shandong Province were similar to Kawasaki strains, but Karp strains also existed. SN - 0254-6450 UR - https://www.unboundmedicine.com/medline/citation/15555396/[Amplification_and_typing_of_Sta56_gene_of_Orientia_tsutsugamushi_from_Shandong_province]_ DB - PRIME DP - Unbound Medicine ER -