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[Construction and identification of anti-chymopapain scFv phage display library].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Nov; 20(6):689-92.XB

Abstract

AIM

To construct phage display library of anti-chymopapain scFv.

METHODS

V(H) and V(L) gene repertoires were amplified from splenocyte mRNA by RT-PCR and joined by a (Gly(4)ser)3 linker to obtain scFv genes. The scFv genes were then cloned into phagemid pFAB5C to construct phage display library. Affinity selection and ELISA were used to identify specific phage antibody to chymopapain.

RESULTS

After 4 rounds of panning, high affinity scFv was obtained.

CONCLUSION

Phage display library of anti-chymopapain scFv was successfully constructed, and scFv with binding ability to chymopapain was obtained.

Authors+Show Affiliations

Department of Bioengineering, South China University of Technology, Guangzhou 510640, China. bestwjp@sohu.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15555436

Citation

Wang, Jian-ping, et al. "[Construction and Identification of Anti-chymopapain scFv Phage Display Library]." Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, vol. 20, no. 6, 2004, pp. 689-92.
Wang JP, Zhao SJ, Wang J, et al. [Construction and identification of anti-chymopapain scFv phage display library]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004;20(6):689-92.
Wang, J. P., Zhao, S. J., Wang, J., Wu, J., Yang, T. C., & Yang, J. (2004). [Construction and identification of anti-chymopapain scFv phage display library]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology, 20(6), 689-92.
Wang JP, et al. [Construction and Identification of Anti-chymopapain scFv Phage Display Library]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004;20(6):689-92. PubMed PMID: 15555436.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Construction and identification of anti-chymopapain scFv phage display library]. AU - Wang,Jian-ping, AU - Zhao,Shu-jin, AU - Wang,Jie, AU - Wu,Jie, AU - Yang,Tai-cheng, AU - Yang,Jing, PY - 2004/11/24/pubmed PY - 2006/4/29/medline PY - 2004/11/24/entrez SP - 689 EP - 92 JF - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology JO - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi VL - 20 IS - 6 N2 - AIM: To construct phage display library of anti-chymopapain scFv. METHODS: V(H) and V(L) gene repertoires were amplified from splenocyte mRNA by RT-PCR and joined by a (Gly(4)ser)3 linker to obtain scFv genes. The scFv genes were then cloned into phagemid pFAB5C to construct phage display library. Affinity selection and ELISA were used to identify specific phage antibody to chymopapain. RESULTS: After 4 rounds of panning, high affinity scFv was obtained. CONCLUSION: Phage display library of anti-chymopapain scFv was successfully constructed, and scFv with binding ability to chymopapain was obtained. SN - 1007-8738 UR - https://www.unboundmedicine.com/medline/citation/15555436/[Construction_and_identification_of_anti_chymopapain_scFv_phage_display_library]_ DB - PRIME DP - Unbound Medicine ER -