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Microchimerism in immune competent patients related to the leukocyte content of transfused red blood cell concentrates.
Transfus Apher Sci. 2004 Dec; 31(3):173-80.TA

Abstract

BACKGROUND

Microchimerism may play a part in transfusion complications. The aim of this study was to examine whether establishment of post-transfusion microchimerism was related to leukocyte content.

METHODS

Twenty non-pregnant female patients, without known malignant or immunological diseases, mean age 68 years, receiving 2-4 units of red blood cell concentrates during elective surgery, were included. One or two of the units were from male donors. Ten patients received buffy-coat depleted red blood cell concentrates, leukocyte count 108-109 per unit, and 10 patients received red blood cells leukoreduced by prestorage leukocyte filtration, with a leukocyte count of <106 per unit. EDTA samples were collected in vacuum tubes before and after 1 week and 6 months after transfusion. The tubes were frozen and stored at -400 degrees C. Genomic DNA was isolated and PCR performed using four primer sets amplifying markers on the Y-chromosome.

RESULTS

Microchimerism was detected in a total of eight out of the 20 patients. In three patients microchimerism was detected only before transfusion. These patients had given birth to one or two boys each, and had no history of previous transfusion. Two patients receiving buffy-coat depleted red blood cell concentrates and two patients receiving leukoreduced red blood cell concentrates had detectable microchimerism 1 week after transfusion. The age of the transfused red blood cell concentrates was 6, 24, 8 and 7 days, respectively. One patient receiving leukoreduced red blood cell concentrates had detectable microchimerism after 6 months. The age of this concentrate was 22 days.

DISCUSSION

This study demonstrates that microchimerism after transfusion does not seem to be dose dependent, and can be induced even by a >3 week old leukoreduced red blood cell concentrate with a very low leukocyte content.

Authors+Show Affiliations

Institute of Immunology, Rikshospitalet University Hospital, Oslo NO-0023, Norway; Blood Bank, Asker and Baerum Hospital, Rud NO-1309, Norway. oystein.flesland@rikshospitalet.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15556463

Citation

Flesland, Oystein, et al. "Microchimerism in Immune Competent Patients Related to the Leukocyte Content of Transfused Red Blood Cell Concentrates." Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis, vol. 31, no. 3, 2004, pp. 173-80.
Flesland O, Ip LS, Storlien AS, et al. Microchimerism in immune competent patients related to the leukocyte content of transfused red blood cell concentrates. Transfus Apher Sci. 2004;31(3):173-80.
Flesland, O., Ip, L. S., Storlien, A. S., Spurkland, A., Larsen, J., & Solheim, B. G. (2004). Microchimerism in immune competent patients related to the leukocyte content of transfused red blood cell concentrates. Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis, 31(3), 173-80.
Flesland O, et al. Microchimerism in Immune Competent Patients Related to the Leukocyte Content of Transfused Red Blood Cell Concentrates. Transfus Apher Sci. 2004;31(3):173-80. PubMed PMID: 15556463.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Microchimerism in immune competent patients related to the leukocyte content of transfused red blood cell concentrates. AU - Flesland,Oystein, AU - Ip,Lisa S K, AU - Storlien,Anne S, AU - Spurkland,Anne, AU - Larsen,Jonn, AU - Solheim,Bjarte G, PY - 2004/06/01/received PY - 2004/07/01/revised PY - 2004/07/01/accepted PY - 2004/11/24/pubmed PY - 2005/2/18/medline PY - 2004/11/24/entrez SP - 173 EP - 80 JF - Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis JO - Transfus Apher Sci VL - 31 IS - 3 N2 - BACKGROUND: Microchimerism may play a part in transfusion complications. The aim of this study was to examine whether establishment of post-transfusion microchimerism was related to leukocyte content. METHODS: Twenty non-pregnant female patients, without known malignant or immunological diseases, mean age 68 years, receiving 2-4 units of red blood cell concentrates during elective surgery, were included. One or two of the units were from male donors. Ten patients received buffy-coat depleted red blood cell concentrates, leukocyte count 108-109 per unit, and 10 patients received red blood cells leukoreduced by prestorage leukocyte filtration, with a leukocyte count of <106 per unit. EDTA samples were collected in vacuum tubes before and after 1 week and 6 months after transfusion. The tubes were frozen and stored at -400 degrees C. Genomic DNA was isolated and PCR performed using four primer sets amplifying markers on the Y-chromosome. RESULTS: Microchimerism was detected in a total of eight out of the 20 patients. In three patients microchimerism was detected only before transfusion. These patients had given birth to one or two boys each, and had no history of previous transfusion. Two patients receiving buffy-coat depleted red blood cell concentrates and two patients receiving leukoreduced red blood cell concentrates had detectable microchimerism 1 week after transfusion. The age of the transfused red blood cell concentrates was 6, 24, 8 and 7 days, respectively. One patient receiving leukoreduced red blood cell concentrates had detectable microchimerism after 6 months. The age of this concentrate was 22 days. DISCUSSION: This study demonstrates that microchimerism after transfusion does not seem to be dose dependent, and can be induced even by a >3 week old leukoreduced red blood cell concentrate with a very low leukocyte content. SN - 1473-0502 UR - https://www.unboundmedicine.com/medline/citation/15556463/Microchimerism_in_immune_competent_patients_related_to_the_leukocyte_content_of_transfused_red_blood_cell_concentrates_ DB - PRIME DP - Unbound Medicine ER -