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Liquid chromatography-mass spectrometry method for determination of tetramethylpyrazine and its metabolite in dog plasma.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 25; 813(1-2):263-8.JC

Abstract

A liquid chromatography-mass spectrometry method is described for the determination of tetramethylpyrazine (TMP) and its active metabolite, 2-hydroxymethyl-3,5,6-trimethylpyrazine (HTMP) in dog plasma. This method involves a plasma clean-up step using protein precipitation procedure followed by LC separation and positive electrospray ionization mass spectrometry detection (ESI-MS). Chromatographic separation of the analytes was achieved on a C18 column using a mobile phase of methanol, water and acetic acid (50:50:0.6, v/v/v) at a flow rate of 1.0 ml/min. Selected ion monitoring (SIM) mode was used for analyte quantitation at m/z 137.2 for TMP, m/z 153.2 for HTMP and m/z 195.2 for caffeine. The linearity was obtained over the concentration ranges of 20-6000 ng/ml for TMP and 20-4000 ng/ml for HTMP and the lower limit of quantitation was 20 ng/ml for both analytes. For each level of QC samples, both inter- and intra-day precisions (R.S.D.) were <or=7.4% for TMP and <or=6.0% for HTMP, and accuracy (R.E.) was +/-6.0% for TMP and <or=3.5% for HTMP. The proposed LC-MS method was successfully applied to the pharmacokinetic studies of a TMP formulation preparation after oral administration to beagle dogs.

Authors+Show Affiliations

School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15556542

Citation

Wang, Peng, et al. "Liquid Chromatography-mass Spectrometry Method for Determination of Tetramethylpyrazine and Its Metabolite in Dog Plasma." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 813, no. 1-2, 2004, pp. 263-8.
Wang P, Jin X, Qi M, et al. Liquid chromatography-mass spectrometry method for determination of tetramethylpyrazine and its metabolite in dog plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;813(1-2):263-8.
Wang, P., Jin, X., Qi, M., & Fang, L. (2004). Liquid chromatography-mass spectrometry method for determination of tetramethylpyrazine and its metabolite in dog plasma. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 813(1-2), 263-8.
Wang P, et al. Liquid Chromatography-mass Spectrometry Method for Determination of Tetramethylpyrazine and Its Metabolite in Dog Plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 25;813(1-2):263-8. PubMed PMID: 15556542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatography-mass spectrometry method for determination of tetramethylpyrazine and its metabolite in dog plasma. AU - Wang,Peng, AU - Jin,Xin, AU - Qi,Meiling, AU - Fang,Lin, PY - 2004/07/30/received PY - 2004/09/23/accepted PY - 2004/11/24/pubmed PY - 2005/4/12/medline PY - 2004/11/24/entrez SP - 263 EP - 8 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 813 IS - 1-2 N2 - A liquid chromatography-mass spectrometry method is described for the determination of tetramethylpyrazine (TMP) and its active metabolite, 2-hydroxymethyl-3,5,6-trimethylpyrazine (HTMP) in dog plasma. This method involves a plasma clean-up step using protein precipitation procedure followed by LC separation and positive electrospray ionization mass spectrometry detection (ESI-MS). Chromatographic separation of the analytes was achieved on a C18 column using a mobile phase of methanol, water and acetic acid (50:50:0.6, v/v/v) at a flow rate of 1.0 ml/min. Selected ion monitoring (SIM) mode was used for analyte quantitation at m/z 137.2 for TMP, m/z 153.2 for HTMP and m/z 195.2 for caffeine. The linearity was obtained over the concentration ranges of 20-6000 ng/ml for TMP and 20-4000 ng/ml for HTMP and the lower limit of quantitation was 20 ng/ml for both analytes. For each level of QC samples, both inter- and intra-day precisions (R.S.D.) were <or=7.4% for TMP and <or=6.0% for HTMP, and accuracy (R.E.) was +/-6.0% for TMP and <or=3.5% for HTMP. The proposed LC-MS method was successfully applied to the pharmacokinetic studies of a TMP formulation preparation after oral administration to beagle dogs. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15556542/Liquid_chromatography_mass_spectrometry_method_for_determination_of_tetramethylpyrazine_and_its_metabolite_in_dog_plasma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(04)00783-4 DB - PRIME DP - Unbound Medicine ER -