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Selective and sensitive liquid chromatography-tandem mass spectrometry method for the determination of levonorgestrel in human plasma.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 25; 813(1-2):331-6.JC

Abstract

A selective, sensitive and rapid liquid chromatography-tandem mass spectrometry method for the determination of levonorgestrel in plasma was developed. An Applied Biosystems API 3000 triple quadrupole mass spectrometer set to multiple reaction monitoring (MRM) mode, using atmospheric pressure photospray ionisation (APPI) in the positive mode. Using 17-alpha-methyltestosterone as internal standard (IS), liquid-liquid extraction was followed by reversed phase liquid chromatography using a phenyl-hexyl column and tandem mass spectrometric detection. The mean recovery for levonorgestrel and 17-alpha-methyltestosterone was 99.5 and 62.9%, respectively. The method was validated from 0.265 to 130 ng levonorgestrel/ml plasma with the lower limit of quantification (LLOQ) set at 0.265 ng/ml. This assay method makes use of the increased sensitivity and selectivity of tandem mass spectrometric (MS/MS) detection, allowing for a rapid (extraction and chromatography) and selective method for the determination of levonorgestrel in human plasma. The assay method was used in a pharmacokinetic study to quantify levonorgestrel in human plasma samples generated after administrating a single oral dose of 1.5 mg levonorgestrel to healthy female volunteers for up to five half lives. The total chromatographic runtime of this method was 5.0 min per sample, allowing for analysis of a large number of samples per batch.

Authors+Show Affiliations

FARMOVS-PAREXEL Clinical Research Organisation, Private Bag X09, Brandhof 9324, South Africa. hendrik.theron@farmovs-parexel.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15556549

Citation

Theron, H B., et al. "Selective and Sensitive Liquid Chromatography-tandem Mass Spectrometry Method for the Determination of Levonorgestrel in Human Plasma." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 813, no. 1-2, 2004, pp. 331-6.
Theron HB, Coetzee C, Sutherland FC, et al. Selective and sensitive liquid chromatography-tandem mass spectrometry method for the determination of levonorgestrel in human plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2004;813(1-2):331-6.
Theron, H. B., Coetzee, C., Sutherland, F. C., Wiesner, J. L., & Swart, K. J. (2004). Selective and sensitive liquid chromatography-tandem mass spectrometry method for the determination of levonorgestrel in human plasma. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 813(1-2), 331-6.
Theron HB, et al. Selective and Sensitive Liquid Chromatography-tandem Mass Spectrometry Method for the Determination of Levonorgestrel in Human Plasma. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 25;813(1-2):331-6. PubMed PMID: 15556549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective and sensitive liquid chromatography-tandem mass spectrometry method for the determination of levonorgestrel in human plasma. AU - Theron,H B, AU - Coetzee,C, AU - Sutherland,F C W, AU - Wiesner,J L, AU - Swart,K J, PY - 2004/07/12/received PY - 2004/10/13/accepted PY - 2004/11/24/pubmed PY - 2005/4/12/medline PY - 2004/11/24/entrez SP - 331 EP - 6 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 813 IS - 1-2 N2 - A selective, sensitive and rapid liquid chromatography-tandem mass spectrometry method for the determination of levonorgestrel in plasma was developed. An Applied Biosystems API 3000 triple quadrupole mass spectrometer set to multiple reaction monitoring (MRM) mode, using atmospheric pressure photospray ionisation (APPI) in the positive mode. Using 17-alpha-methyltestosterone as internal standard (IS), liquid-liquid extraction was followed by reversed phase liquid chromatography using a phenyl-hexyl column and tandem mass spectrometric detection. The mean recovery for levonorgestrel and 17-alpha-methyltestosterone was 99.5 and 62.9%, respectively. The method was validated from 0.265 to 130 ng levonorgestrel/ml plasma with the lower limit of quantification (LLOQ) set at 0.265 ng/ml. This assay method makes use of the increased sensitivity and selectivity of tandem mass spectrometric (MS/MS) detection, allowing for a rapid (extraction and chromatography) and selective method for the determination of levonorgestrel in human plasma. The assay method was used in a pharmacokinetic study to quantify levonorgestrel in human plasma samples generated after administrating a single oral dose of 1.5 mg levonorgestrel to healthy female volunteers for up to five half lives. The total chromatographic runtime of this method was 5.0 min per sample, allowing for analysis of a large number of samples per batch. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/15556549/Selective_and_sensitive_liquid_chromatography_tandem_mass_spectrometry_method_for_the_determination_of_levonorgestrel_in_human_plasma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(04)00829-3 DB - PRIME DP - Unbound Medicine ER -