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Diversity and abundance of Bacteria and Archaea in the Bor Khlueng Hot Spring in Thailand.
J Basic Microbiol. 2004; 44(6):430-44.JB

Abstract

The prokaryotic diversity in the Bor Khlueng hot spring in Ratchaburi province, Thailand was investigated by a culture-independent molecular approach. This hydrothermal pool is located in the central part of Thailand and contains sulfide-rich mineral water that is believed to relieve muscle ache and pain. The water flow year-round with temperature ranging between 50-57 degrees C. Community DNA was extracted directly from sediment samples by coring to depth of approximately 20-30 cm. Small-subunit rRNA genes (16S rDNA) were amplified by PCR using primers specific for the domains Archaea and Bacteria. The PCR products were cloned and sequenced. For the bacterial rDNA clone library, 200 clones were randomly selected for further analyses. After restriction fragment length polymorphism (RFLP) analysis of rDNA clones and exclusion of chimeric sequences 36 phylotypes were obtained. The Bor Khlueng phylotypes spanned a wide range within the domain Bacteria, occupying eleven major lineages (phyla). Almost a quarter (23%) of the clones were classified as Acidobacteria. The other clones were grouped into the Bacteriodetes (19%), Nitrospirae (13%), Proteobacteria (12%), Deinococcus-Thermus lineage (11%), planctomycetes (6%), and Verrucomicrobia (5%). The four remaining phyla, 5% each, were assigned to Actinobacteria, Chloroflexi, Cyanobacteria, and the candidate division "OP10". For the archaeal 16S rRNA gene sequence library, 25 distinct phylotypes were obtained, 17 clones were found to be associated with Crenarahaeota and 8 clones were associated with Euryarachaeota. The findings of the molecular survey of this so far not investigated site showed that Bor Khlueng hot spring is a potential rich source of unique bacterial and archaeal species. The great majority (approximately 80%) of the prokaryotic sequences detected in Bor Khlueng were unknown.

Authors+Show Affiliations

BIOTEC Center Laboratory, Thailand Science Park, 113 Paholyothin Road, Klong 1, Klongluang, Pathumthani 12120, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15558824

Citation

Kanokratana, Pattanop, et al. "Diversity and Abundance of Bacteria and Archaea in the Bor Khlueng Hot Spring in Thailand." Journal of Basic Microbiology, vol. 44, no. 6, 2004, pp. 430-44.
Kanokratana P, Chanapan S, Pootanakit K, et al. Diversity and abundance of Bacteria and Archaea in the Bor Khlueng Hot Spring in Thailand. J Basic Microbiol. 2004;44(6):430-44.
Kanokratana, P., Chanapan, S., Pootanakit, K., & Eurwilaichitr, L. (2004). Diversity and abundance of Bacteria and Archaea in the Bor Khlueng Hot Spring in Thailand. Journal of Basic Microbiology, 44(6), 430-44.
Kanokratana P, et al. Diversity and Abundance of Bacteria and Archaea in the Bor Khlueng Hot Spring in Thailand. J Basic Microbiol. 2004;44(6):430-44. PubMed PMID: 15558824.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diversity and abundance of Bacteria and Archaea in the Bor Khlueng Hot Spring in Thailand. AU - Kanokratana,Pattanop, AU - Chanapan,Supavadee, AU - Pootanakit,Kusol, AU - Eurwilaichitr,Lily, PY - 2004/11/24/pubmed PY - 2005/1/28/medline PY - 2004/11/24/entrez SP - 430 EP - 44 JF - Journal of basic microbiology JO - J Basic Microbiol VL - 44 IS - 6 N2 - The prokaryotic diversity in the Bor Khlueng hot spring in Ratchaburi province, Thailand was investigated by a culture-independent molecular approach. This hydrothermal pool is located in the central part of Thailand and contains sulfide-rich mineral water that is believed to relieve muscle ache and pain. The water flow year-round with temperature ranging between 50-57 degrees C. Community DNA was extracted directly from sediment samples by coring to depth of approximately 20-30 cm. Small-subunit rRNA genes (16S rDNA) were amplified by PCR using primers specific for the domains Archaea and Bacteria. The PCR products were cloned and sequenced. For the bacterial rDNA clone library, 200 clones were randomly selected for further analyses. After restriction fragment length polymorphism (RFLP) analysis of rDNA clones and exclusion of chimeric sequences 36 phylotypes were obtained. The Bor Khlueng phylotypes spanned a wide range within the domain Bacteria, occupying eleven major lineages (phyla). Almost a quarter (23%) of the clones were classified as Acidobacteria. The other clones were grouped into the Bacteriodetes (19%), Nitrospirae (13%), Proteobacteria (12%), Deinococcus-Thermus lineage (11%), planctomycetes (6%), and Verrucomicrobia (5%). The four remaining phyla, 5% each, were assigned to Actinobacteria, Chloroflexi, Cyanobacteria, and the candidate division "OP10". For the archaeal 16S rRNA gene sequence library, 25 distinct phylotypes were obtained, 17 clones were found to be associated with Crenarahaeota and 8 clones were associated with Euryarachaeota. The findings of the molecular survey of this so far not investigated site showed that Bor Khlueng hot spring is a potential rich source of unique bacterial and archaeal species. The great majority (approximately 80%) of the prokaryotic sequences detected in Bor Khlueng were unknown. SN - 0233-111X UR - https://www.unboundmedicine.com/medline/citation/15558824/Diversity_and_abundance_of_Bacteria_and_Archaea_in_the_Bor_Khlueng_Hot_Spring_in_Thailand_ L2 - https://doi.org/10.1002/jobm.200410388 DB - PRIME DP - Unbound Medicine ER -