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Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis).
J Exp Zool A Comp Exp Biol. 2004 Dec 01; 301(12):979-91.JE

Abstract

The time course of osmoregulatory adjustments and expressional changes of three key ion transporters in the gill were investigated in the striped bass during salinity acclimations. In three experiments, fish were transferred from fresh water (FW) to seawater (SW), from SW to FW, and from 15-ppt brackish water (BW) to either FW or SW, respectively. Each transfer induced minor deflections in serum [Na+] and muscle water content, both being corrected rapidly (24 hr). Transfer from FW to SW increased gill Na+,K+-ATPase activity and Na+,K+,2Cl- co-transporter expression after 3 days. Abundance of Na+,K+-ATPase alpha-subunit mRNA and protein was unchanged. Changes in Na+,K+,2Cl- co-transporter protein were preceded by increased mRNA expression after 24 hr. Expression of V-type H+-ATPase mRNA decreased after 3 days. Transfer from SW to FW induced no change in expression of gill Na+,K+-ATPase. However, Na+,K+,2Cl- co-transporter mRNA and protein levels decreased after 24 hr and 7 days, respectively. Expression of H+-ATPase mRNA increased in response to FW after 7 days. In BW fish transferred to FW and SW, gill Na+,K+-ATPase activity was stimulated by both challenges, suggesting both a hyper- and a hypo-osmoregulatory response of the enzyme. Acclimation of striped bass to SW occurs on a rapid time scale. This seems partly to rely on the relative high abundance of gill Na+,K+-ATPase and Na+,K+,2Cl- co-transporter in FW fish. In a separate study, we found a smaller response to SW in expression of these ion transport proteins in striped bass when compared with the less euryhaline brown trout. In both FW and SW, NEM-sensitive gill H+-ATPase activity was negligible in striped bass and approximately 10-fold higher in brown trout. This suggests that in striped bass Na+-uptake in FW may rely more on a relatively high abundance/activity of Na+,K+-ATPase compared to trout, where H+-ATPase is critical for establishing a thermodynamically favorable gradient for Na+-uptake.

Authors+Show Affiliations

Department of Zoology, North Carolina State University, Raleigh, North Carolina 27695-7617, USA. christian_tipsmark@ncsu.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15562450

Citation

Tipsmark, Christian Kølbaek, et al. "Effect of Salinity On Expression of Branchial Ion Transporters in Striped Bass (Morone Saxatilis)." Journal of Experimental Zoology. Part A, Comparative Experimental Biology, vol. 301, no. 12, 2004, pp. 979-91.
Tipsmark CK, Madsen SS, Borski RJ. Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis). J Exp Zool A Comp Exp Biol. 2004;301(12):979-91.
Tipsmark, C. K., Madsen, S. S., & Borski, R. J. (2004). Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis). Journal of Experimental Zoology. Part A, Comparative Experimental Biology, 301(12), 979-91.
Tipsmark CK, Madsen SS, Borski RJ. Effect of Salinity On Expression of Branchial Ion Transporters in Striped Bass (Morone Saxatilis). J Exp Zool A Comp Exp Biol. 2004 Dec 1;301(12):979-91. PubMed PMID: 15562450.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of salinity on expression of branchial ion transporters in striped bass (Morone saxatilis). AU - Tipsmark,Christian Kølbaek, AU - Madsen,Steffen Søndergaard, AU - Borski,Russell John, PY - 2004/11/25/pubmed PY - 2005/3/22/medline PY - 2004/11/25/entrez SP - 979 EP - 91 JF - Journal of experimental zoology. Part A, Comparative experimental biology JO - J Exp Zool A Comp Exp Biol VL - 301 IS - 12 N2 - The time course of osmoregulatory adjustments and expressional changes of three key ion transporters in the gill were investigated in the striped bass during salinity acclimations. In three experiments, fish were transferred from fresh water (FW) to seawater (SW), from SW to FW, and from 15-ppt brackish water (BW) to either FW or SW, respectively. Each transfer induced minor deflections in serum [Na+] and muscle water content, both being corrected rapidly (24 hr). Transfer from FW to SW increased gill Na+,K+-ATPase activity and Na+,K+,2Cl- co-transporter expression after 3 days. Abundance of Na+,K+-ATPase alpha-subunit mRNA and protein was unchanged. Changes in Na+,K+,2Cl- co-transporter protein were preceded by increased mRNA expression after 24 hr. Expression of V-type H+-ATPase mRNA decreased after 3 days. Transfer from SW to FW induced no change in expression of gill Na+,K+-ATPase. However, Na+,K+,2Cl- co-transporter mRNA and protein levels decreased after 24 hr and 7 days, respectively. Expression of H+-ATPase mRNA increased in response to FW after 7 days. In BW fish transferred to FW and SW, gill Na+,K+-ATPase activity was stimulated by both challenges, suggesting both a hyper- and a hypo-osmoregulatory response of the enzyme. Acclimation of striped bass to SW occurs on a rapid time scale. This seems partly to rely on the relative high abundance of gill Na+,K+-ATPase and Na+,K+,2Cl- co-transporter in FW fish. In a separate study, we found a smaller response to SW in expression of these ion transport proteins in striped bass when compared with the less euryhaline brown trout. In both FW and SW, NEM-sensitive gill H+-ATPase activity was negligible in striped bass and approximately 10-fold higher in brown trout. This suggests that in striped bass Na+-uptake in FW may rely more on a relatively high abundance/activity of Na+,K+-ATPase compared to trout, where H+-ATPase is critical for establishing a thermodynamically favorable gradient for Na+-uptake. SN - 1548-8969 UR - https://www.unboundmedicine.com/medline/citation/15562450/Effect_of_salinity_on_expression_of_branchial_ion_transporters_in_striped_bass__Morone_saxatilis__ L2 - https://doi.org/10.1002/jez.a.119 DB - PRIME DP - Unbound Medicine ER -