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High-power blue/UV light-emitting diodes as excitation sources for sensitive detection.
Electrophoresis. 2004 Nov; 25(21-22):3796-804.E

Abstract

With advances in III-V nitride manufacturing processes, high-power light-emitting diode (LED) chips in the blue and UV wavelengths are now commercially available at reasonable cost and can be used as excitation sources in optical sensing. We describe the use of these high-power blue and UV LEDs for sensitive fluorescence detection, including chip-based flow cytometry, capillary electrophoresis (CE), and single-molecule imaging. By using a blue LED with a focusable power of approximately 40 mW as the excitation source for fluorescent beads, we demonstrate a simple chip-based bead sorter capable of enriching the concentration of green fluorescent beads from 63% to 95%. In CE experiments, we show that a mixture of analyte solution containing 30 nM 6-carboxyrhodamine 6G and 10 nM fluorescein can be separated and detected with excellent signal-to-noise ratio (approximately 17 for 10 nM fluorescein) using the collimated emission from a blue LED; the estimated mass detection limit was approximately 200 zmol for fluorescein. We also demonstrated ultrasensitive fluorescence imaging of single rhodamine 123 molecules and individual lambda-DNA molecules. At a small fraction of the cost of an Ar+ laser, high-power blue and UV LEDs are effective alternatives for lasers and arc lamps in fluorescence applications that demand portability, low cost, and convenience.

Authors+Show Affiliations

Department of Chemistry, University of Washington, Seattle, WA 98195, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15565689

Citation

Kuo, Jason S., et al. "High-power blue/UV Light-emitting Diodes as Excitation Sources for Sensitive Detection." Electrophoresis, vol. 25, no. 21-22, 2004, pp. 3796-804.
Kuo JS, Kuyper CL, Allen PB, et al. High-power blue/UV light-emitting diodes as excitation sources for sensitive detection. Electrophoresis. 2004;25(21-22):3796-804.
Kuo, J. S., Kuyper, C. L., Allen, P. B., Fiorini, G. S., & Chiu, D. T. (2004). High-power blue/UV light-emitting diodes as excitation sources for sensitive detection. Electrophoresis, 25(21-22), 3796-804.
Kuo JS, et al. High-power blue/UV Light-emitting Diodes as Excitation Sources for Sensitive Detection. Electrophoresis. 2004;25(21-22):3796-804. PubMed PMID: 15565689.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-power blue/UV light-emitting diodes as excitation sources for sensitive detection. AU - Kuo,Jason S, AU - Kuyper,Christopher L, AU - Allen,Peter B, AU - Fiorini,Gina S, AU - Chiu,Daniel T, PY - 2004/11/27/pubmed PY - 2005/4/20/medline PY - 2004/11/27/entrez SP - 3796 EP - 804 JF - Electrophoresis JO - Electrophoresis VL - 25 IS - 21-22 N2 - With advances in III-V nitride manufacturing processes, high-power light-emitting diode (LED) chips in the blue and UV wavelengths are now commercially available at reasonable cost and can be used as excitation sources in optical sensing. We describe the use of these high-power blue and UV LEDs for sensitive fluorescence detection, including chip-based flow cytometry, capillary electrophoresis (CE), and single-molecule imaging. By using a blue LED with a focusable power of approximately 40 mW as the excitation source for fluorescent beads, we demonstrate a simple chip-based bead sorter capable of enriching the concentration of green fluorescent beads from 63% to 95%. In CE experiments, we show that a mixture of analyte solution containing 30 nM 6-carboxyrhodamine 6G and 10 nM fluorescein can be separated and detected with excellent signal-to-noise ratio (approximately 17 for 10 nM fluorescein) using the collimated emission from a blue LED; the estimated mass detection limit was approximately 200 zmol for fluorescein. We also demonstrated ultrasensitive fluorescence imaging of single rhodamine 123 molecules and individual lambda-DNA molecules. At a small fraction of the cost of an Ar+ laser, high-power blue and UV LEDs are effective alternatives for lasers and arc lamps in fluorescence applications that demand portability, low cost, and convenience. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/15565689/High_power_blue/UV_light_emitting_diodes_as_excitation_sources_for_sensitive_detection_ DB - PRIME DP - Unbound Medicine ER -