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Identification and characterization of a DNA photolyase-containing baculovirus from Chrysodeixis chalcites.
Virology 2004; 330(2):460-70V

Abstract

A hitherto unknown single nucleocapsid nucleopolyhedrovirus (SNPV) with a unique property was isolated from larvae of the looper Chrysodeixis chalcites (Lepidoptera, Noctuidae, Plusiinae). Polyhedrin, lef-8, and pif-2 gene sequences were obtained by PCR with degenerate primers and used for phylogenetic analysis. ChchNPV belonged to class II NPVs and its polyhedrin sequence was most similar to that of class II NPVs of other members of the subfamily Plusiinae. Further genetic characterization involved the random cloning of HindIII fragments into a plasmid vector and analysis by end-in sequencing. A gene so far unique to baculoviruses was identified, which encodes a putative DNA repair enzyme: cyclobutane pyrimidine dimer (CPD) DNA photolyase (dpl). The transcriptional activity of this gene was demonstrated in both ChchNPV-infected C. chalcites larvae and infected Trichoplusia ni High Five cells by RT-PCR and 5' and 3' RACE analysis. The possible role of this gene in the biology of the virus is discussed.

Authors+Show Affiliations

Laboratory of Virology, Wageningen University, 6709 PD Wageningen, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15567439

Citation

van Oers, Monique M., et al. "Identification and Characterization of a DNA Photolyase-containing Baculovirus From Chrysodeixis Chalcites." Virology, vol. 330, no. 2, 2004, pp. 460-70.
van Oers MM, Herniou EA, Usmany M, et al. Identification and characterization of a DNA photolyase-containing baculovirus from Chrysodeixis chalcites. Virology. 2004;330(2):460-70.
van Oers, M. M., Herniou, E. A., Usmany, M., Messelink, G. J., & Vlak, J. M. (2004). Identification and characterization of a DNA photolyase-containing baculovirus from Chrysodeixis chalcites. Virology, 330(2), pp. 460-70.
van Oers MM, et al. Identification and Characterization of a DNA Photolyase-containing Baculovirus From Chrysodeixis Chalcites. Virology. 2004 Dec 20;330(2):460-70. PubMed PMID: 15567439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of a DNA photolyase-containing baculovirus from Chrysodeixis chalcites. AU - van Oers,Monique M, AU - Herniou,Elisabeth A, AU - Usmany,Magda, AU - Messelink,Gerben J, AU - Vlak,Just M, PY - 2004/08/27/received PY - 2004/09/20/revised PY - 2004/09/22/accepted PY - 2004/11/30/pubmed PY - 2005/1/12/medline PY - 2004/11/30/entrez SP - 460 EP - 70 JF - Virology JO - Virology VL - 330 IS - 2 N2 - A hitherto unknown single nucleocapsid nucleopolyhedrovirus (SNPV) with a unique property was isolated from larvae of the looper Chrysodeixis chalcites (Lepidoptera, Noctuidae, Plusiinae). Polyhedrin, lef-8, and pif-2 gene sequences were obtained by PCR with degenerate primers and used for phylogenetic analysis. ChchNPV belonged to class II NPVs and its polyhedrin sequence was most similar to that of class II NPVs of other members of the subfamily Plusiinae. Further genetic characterization involved the random cloning of HindIII fragments into a plasmid vector and analysis by end-in sequencing. A gene so far unique to baculoviruses was identified, which encodes a putative DNA repair enzyme: cyclobutane pyrimidine dimer (CPD) DNA photolyase (dpl). The transcriptional activity of this gene was demonstrated in both ChchNPV-infected C. chalcites larvae and infected Trichoplusia ni High Five cells by RT-PCR and 5' and 3' RACE analysis. The possible role of this gene in the biology of the virus is discussed. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/15567439/Identification_and_characterization_of_a_DNA_photolyase_containing_baculovirus_from_Chrysodeixis_chalcites_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(04)00643-9 DB - PRIME DP - Unbound Medicine ER -