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Extrasynaptic localization of GABA in the developing mouse cerebellum.
Neurosci Res 2004; 50(4):447-58NR

Abstract

In the adult brain, gamma-amino butyric acid (GABA) is synaptically released and mediates inhibitory transmission. Recent studies have revealed that GABA is a trophic factor for brain development. To reveal the distribution of GABA and its secretion mechanisms during brain development, we investigated the immunohistochemical localization of two molecules, GABA and vesicular GABA transporter (VGAT), which is a GABAergic vesicle protein, in the developing mouse cerebellum by means of newly developed antibodies. Furthermore, we tested the relationship between developmental changes in distribution of above two molecules in the presynapses and ontogeny of GABAergic synapses. GABAergic synapses were detected by immunohistochemistry for the GABA(A) receptor alpha1 subunit, which is an essential subunit for inhibitory synaptic transmission in the mature cerebellar cortex. Until postnatal day 7 (P7), GABA was localized throughout the GABAergic neurons, and VGAT accumulated at axon varicosities and growth cones, where the alpha1 subunit did not accumulate. After P10, both GABA and VGAT became confined to the terminal sites where the alpha1 subunit was localized. These results suggested that GABA was extrasynaptically released from axon varicosities and growth cones by vesicular secretion 'exocytosis' and from all parts of GABAergic neurons during the cerebellar development by non-vesicular secretion 'diacrine'.

Authors+Show Affiliations

Department of Molecular Neuroanatomy, Hokkaido University School of Medicine, Kita-15 Nishi-7, Kita-Ku, Sapporo 060 8638, Japan. takachan@med.hokudai.ac.jpNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15567482

Citation

Takayama, Chitoshi, and Yoshiro Inoue. "Extrasynaptic Localization of GABA in the Developing Mouse Cerebellum." Neuroscience Research, vol. 50, no. 4, 2004, pp. 447-58.
Takayama C, Inoue Y. Extrasynaptic localization of GABA in the developing mouse cerebellum. Neurosci Res. 2004;50(4):447-58.
Takayama, C., & Inoue, Y. (2004). Extrasynaptic localization of GABA in the developing mouse cerebellum. Neuroscience Research, 50(4), pp. 447-58.
Takayama C, Inoue Y. Extrasynaptic Localization of GABA in the Developing Mouse Cerebellum. Neurosci Res. 2004;50(4):447-58. PubMed PMID: 15567482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extrasynaptic localization of GABA in the developing mouse cerebellum. AU - Takayama,Chitoshi, AU - Inoue,Yoshiro, PY - 2004/05/14/received PY - 2004/08/17/accepted PY - 2004/11/30/pubmed PY - 2005/3/16/medline PY - 2004/11/30/entrez SP - 447 EP - 58 JF - Neuroscience research JO - Neurosci. Res. VL - 50 IS - 4 N2 - In the adult brain, gamma-amino butyric acid (GABA) is synaptically released and mediates inhibitory transmission. Recent studies have revealed that GABA is a trophic factor for brain development. To reveal the distribution of GABA and its secretion mechanisms during brain development, we investigated the immunohistochemical localization of two molecules, GABA and vesicular GABA transporter (VGAT), which is a GABAergic vesicle protein, in the developing mouse cerebellum by means of newly developed antibodies. Furthermore, we tested the relationship between developmental changes in distribution of above two molecules in the presynapses and ontogeny of GABAergic synapses. GABAergic synapses were detected by immunohistochemistry for the GABA(A) receptor alpha1 subunit, which is an essential subunit for inhibitory synaptic transmission in the mature cerebellar cortex. Until postnatal day 7 (P7), GABA was localized throughout the GABAergic neurons, and VGAT accumulated at axon varicosities and growth cones, where the alpha1 subunit did not accumulate. After P10, both GABA and VGAT became confined to the terminal sites where the alpha1 subunit was localized. These results suggested that GABA was extrasynaptically released from axon varicosities and growth cones by vesicular secretion 'exocytosis' and from all parts of GABAergic neurons during the cerebellar development by non-vesicular secretion 'diacrine'. SN - 0168-0102 UR - https://www.unboundmedicine.com/medline/citation/15567482/Extrasynaptic_localization_of_GABA_in_the_developing_mouse_cerebellum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-0102(04)00214-7 DB - PRIME DP - Unbound Medicine ER -