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Role of Bcl-2 family of proteins in mediating apoptotic death of PC12 cells exposed to oxygen and glucose deprivation.
Neurochem Int. 2005 Jan; 46(1):73-81.NI

Abstract

Apoptotic cell death has been observed in many in vivo and in vitro models of ischemia. However, the molecular pathways involved in ischemia-induced apoptosis remain unclear. We have examined the role of Bcl-2 family of proteins in mediating apoptosis of PC12 cells exposed to the conditions of oxygen and glucose deprivation (OGD) or OGD followed by restoration of oxygen and glucose (OGD-restoration, OGD-R). OGD decreased mitochondrial membrane potential and induced necrosis of PC12 cells, which were both prevented by the overexpression of Bcl-2 proteins. OGD-R caused apoptotic cell death, induced cytochrome C release from mitochondria and caspase-3 activation, decreased mitochondrial membrane potential, and increased levels of pro-apoptotic Bax translocated to the mitochondrial membrane, all of which were reversed by overexpression of Bcl-2. These results demonstrate that the cell death induced by OGD and OGD-R in PC12 cells is potentially mediated through the regulation of mitochondrial membrane potential by the Bcl-2 family of proteins. It also reveals the importance of developing therapeutic strategies for maintaining the mitochondrial membrane potential as a possible way of reducing necrotic and apoptotic cell death that occurs following an ischemic insult.

Authors+Show Affiliations

William T. Gossett Neurology Laboratories, Detroit, MI 48202, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15567517

Citation

Koubi, David, et al. "Role of Bcl-2 Family of Proteins in Mediating Apoptotic Death of PC12 Cells Exposed to Oxygen and Glucose Deprivation." Neurochemistry International, vol. 46, no. 1, 2005, pp. 73-81.
Koubi D, Jiang H, Zhang L, et al. Role of Bcl-2 family of proteins in mediating apoptotic death of PC12 cells exposed to oxygen and glucose deprivation. Neurochem Int. 2005;46(1):73-81.
Koubi, D., Jiang, H., Zhang, L., Tang, W., Kuo, J., Rodriguez, A. I., Hunter, T. J., Seidman, M. D., Corcoran, G. B., & Levine, R. A. (2005). Role of Bcl-2 family of proteins in mediating apoptotic death of PC12 cells exposed to oxygen and glucose deprivation. Neurochemistry International, 46(1), 73-81.
Koubi D, et al. Role of Bcl-2 Family of Proteins in Mediating Apoptotic Death of PC12 Cells Exposed to Oxygen and Glucose Deprivation. Neurochem Int. 2005;46(1):73-81. PubMed PMID: 15567517.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of Bcl-2 family of proteins in mediating apoptotic death of PC12 cells exposed to oxygen and glucose deprivation. AU - Koubi,David, AU - Jiang,Hao, AU - Zhang,Lijie, AU - Tang,Wenxue, AU - Kuo,Jarret, AU - Rodriguez,Alba I, AU - Hunter,Tangella Jackson, AU - Seidman,Michael D, AU - Corcoran,George B, AU - Levine,Robert A, PY - 2003/09/18/received PY - 2004/05/11/revised PY - 2004/06/10/accepted PY - 2004/11/30/pubmed PY - 2005/2/23/medline PY - 2004/11/30/entrez SP - 73 EP - 81 JF - Neurochemistry international JO - Neurochem. Int. VL - 46 IS - 1 N2 - Apoptotic cell death has been observed in many in vivo and in vitro models of ischemia. However, the molecular pathways involved in ischemia-induced apoptosis remain unclear. We have examined the role of Bcl-2 family of proteins in mediating apoptosis of PC12 cells exposed to the conditions of oxygen and glucose deprivation (OGD) or OGD followed by restoration of oxygen and glucose (OGD-restoration, OGD-R). OGD decreased mitochondrial membrane potential and induced necrosis of PC12 cells, which were both prevented by the overexpression of Bcl-2 proteins. OGD-R caused apoptotic cell death, induced cytochrome C release from mitochondria and caspase-3 activation, decreased mitochondrial membrane potential, and increased levels of pro-apoptotic Bax translocated to the mitochondrial membrane, all of which were reversed by overexpression of Bcl-2. These results demonstrate that the cell death induced by OGD and OGD-R in PC12 cells is potentially mediated through the regulation of mitochondrial membrane potential by the Bcl-2 family of proteins. It also reveals the importance of developing therapeutic strategies for maintaining the mitochondrial membrane potential as a possible way of reducing necrotic and apoptotic cell death that occurs following an ischemic insult. SN - 0197-0186 UR - https://www.unboundmedicine.com/medline/citation/15567517/Role_of_Bcl_2_family_of_proteins_in_mediating_apoptotic_death_of_PC12_cells_exposed_to_oxygen_and_glucose_deprivation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0197-0186(04)00123-8 DB - PRIME DP - Unbound Medicine ER -