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[The occurrence of Coxiella burnetii in sheep and ticks of the genus Dermacentor in Baden-Wuerttemberg].
Dtsch Tierarztl Wochenschr. 2004 Oct; 111(10):390-4.DT

Abstract

This study examined the occurrence of Coxiella burnetii (C. burnetii), the infectious agent of Q-fever, in sheep and sheep-ticks in Baden-Wuerttemberg, Germany, as a possible source of infection in Q-fever outbreaks. Using PCR, we examined a total of 1066 Dermacentor ticks from 23 herds and 49 samples of tick excrement from 18 herds for C. burnetii. We found the infectious agent in one non-engorged tick and in one sample of tick excrement from the same herd, in Efringen-Kirchen (district Loerrach). Sequencing the PCR-products confirmed the amplifications as specific for C. burnetii. Further serological tests of random samples of the four districts of Baden-Wuerttemberg showed a seroprevalence from 0 to 1.4% using complement fixation test (CFT), as well as a 0.9 to 10.2% seroprevalence, using ELISA test. Serum samples from a Q-fever-suspicious herd resulted, however, in 6% (CFT) and 53% (ELISA) positive reactions. A comparison between CFT and ELISA showed both a correlation of the two test methods that increased with higher CFT titration levels and positive reactions using ELISA for 9.4% of the serums that had tested negative using CFT. The results of the present study reveal that ticks and their excrements are important vectors of transmission of Q-fever in Baden-Wuerttemberg. Investigations on C. burnetii using PCR as well as serological surveys of sheep are important instruments for diagnosis and disease control of Q-fever.

Authors+Show Affiliations

Chemisches und Veterinaruntersuchungsamt Stuttgart, Fellbach. reinhard.sting@CVUAS.BWL.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

ger

PubMed ID

15568636

Citation

Sting, R, et al. "[The Occurrence of Coxiella Burnetii in Sheep and Ticks of the Genus Dermacentor in Baden-Wuerttemberg]." DTW. Deutsche Tierarztliche Wochenschrift, vol. 111, no. 10, 2004, pp. 390-4.
Sting R, Breitling N, Oehme R, et al. [The occurrence of Coxiella burnetii in sheep and ticks of the genus Dermacentor in Baden-Wuerttemberg]. Dtsch Tierarztl Wochenschr. 2004;111(10):390-4.
Sting, R., Breitling, N., Oehme, R., & Kimmig, P. (2004). [The occurrence of Coxiella burnetii in sheep and ticks of the genus Dermacentor in Baden-Wuerttemberg]. DTW. Deutsche Tierarztliche Wochenschrift, 111(10), 390-4.
Sting R, et al. [The Occurrence of Coxiella Burnetii in Sheep and Ticks of the Genus Dermacentor in Baden-Wuerttemberg]. Dtsch Tierarztl Wochenschr. 2004;111(10):390-4. PubMed PMID: 15568636.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The occurrence of Coxiella burnetii in sheep and ticks of the genus Dermacentor in Baden-Wuerttemberg]. AU - Sting,R, AU - Breitling,N, AU - Oehme,R, AU - Kimmig,P, PY - 2004/12/1/pubmed PY - 2005/1/11/medline PY - 2004/12/1/entrez SP - 390 EP - 4 JF - DTW. Deutsche tierarztliche Wochenschrift JO - Dtsch Tierarztl Wochenschr VL - 111 IS - 10 N2 - This study examined the occurrence of Coxiella burnetii (C. burnetii), the infectious agent of Q-fever, in sheep and sheep-ticks in Baden-Wuerttemberg, Germany, as a possible source of infection in Q-fever outbreaks. Using PCR, we examined a total of 1066 Dermacentor ticks from 23 herds and 49 samples of tick excrement from 18 herds for C. burnetii. We found the infectious agent in one non-engorged tick and in one sample of tick excrement from the same herd, in Efringen-Kirchen (district Loerrach). Sequencing the PCR-products confirmed the amplifications as specific for C. burnetii. Further serological tests of random samples of the four districts of Baden-Wuerttemberg showed a seroprevalence from 0 to 1.4% using complement fixation test (CFT), as well as a 0.9 to 10.2% seroprevalence, using ELISA test. Serum samples from a Q-fever-suspicious herd resulted, however, in 6% (CFT) and 53% (ELISA) positive reactions. A comparison between CFT and ELISA showed both a correlation of the two test methods that increased with higher CFT titration levels and positive reactions using ELISA for 9.4% of the serums that had tested negative using CFT. The results of the present study reveal that ticks and their excrements are important vectors of transmission of Q-fever in Baden-Wuerttemberg. Investigations on C. burnetii using PCR as well as serological surveys of sheep are important instruments for diagnosis and disease control of Q-fever. SN - 0341-6593 UR - https://www.unboundmedicine.com/medline/citation/15568636/[The_occurrence_of_Coxiella_burnetii_in_sheep_and_ticks_of_the_genus_Dermacentor_in_Baden_Wuerttemberg]_ DB - PRIME DP - Unbound Medicine ER -