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Curcumin protects mitochondria from oxidative damage and attenuates apoptosis in cortical neurons.
Acta Pharmacol Sin. 2004 Dec; 25(12):1606-12.AP

Abstract

AIM

To investigate the effect of curcumin on tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in rat cortical neurons and to explore the possible mechanism.

METHODS

Primary cultured rat cortical neurons were performed in vitro and cell viability was measured by MTT assay. DNA fragmentation was used to evaluate cell apoptosis. Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (Deltapsim) was determined by flow cytometric assay. Cellular glutathione (GSH) content was measured by spectrophotometer. Bcl-2 family proteins, cytochrome c, cleaved caspase-3, and poly (ADP-ribose) polymerase (PARP) were detected by Western blot.

RESULTS

Exposure of tBHP 100 micromol/L to neurons for 60 min resulted in DYm loss and cytochrome c release from mitochondria and subsequent activation of caspase-3 and PARP cleavation, and cell apoptosis. After removal of tBHP and then further treatment with curcumin (2.5-20 micromol/L) for 18 h, curcumin abrogated Deltapsim loss and cytochrome c release, blocked activation of caspase 3, and altered the expression of Bcl-2 family. Further curcumin treatment also prevented cellular GSH and decreased intracellular ROS generation markedly. Curcumin eventually attenuated tBHP-induced apoptosis in cortical neurons.

CONCLUSION

Curcumin may attenuate oxidative damages in cortical neurons by reducing intracellular production of ROS and protecting mitochondria from oxidative damage.

Authors+Show Affiliations

Fujian Institute of Geriatrics, Fujian Institute of Hematology, Union Hospital, Fujian Medical University, Fuzhou 350001, China. yuangui_zhu@rush.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15569404

Citation

Zhu, Yuan-gui, et al. "Curcumin Protects Mitochondria From Oxidative Damage and Attenuates Apoptosis in Cortical Neurons." Acta Pharmacologica Sinica, vol. 25, no. 12, 2004, pp. 1606-12.
Zhu YG, Chen XC, Chen ZZ, et al. Curcumin protects mitochondria from oxidative damage and attenuates apoptosis in cortical neurons. Acta Pharmacol Sin. 2004;25(12):1606-12.
Zhu, Y. G., Chen, X. C., Chen, Z. Z., Zeng, Y. Q., Shi, G. B., Su, Y. H., & Peng, X. (2004). Curcumin protects mitochondria from oxidative damage and attenuates apoptosis in cortical neurons. Acta Pharmacologica Sinica, 25(12), 1606-12.
Zhu YG, et al. Curcumin Protects Mitochondria From Oxidative Damage and Attenuates Apoptosis in Cortical Neurons. Acta Pharmacol Sin. 2004;25(12):1606-12. PubMed PMID: 15569404.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Curcumin protects mitochondria from oxidative damage and attenuates apoptosis in cortical neurons. AU - Zhu,Yuan-gui, AU - Chen,Xiao-chun, AU - Chen,Zhi-zhe, AU - Zeng,Yu-qi, AU - Shi,Guang-bin, AU - Su,Yan-hua, AU - Peng,Xu, PY - 2004/12/1/pubmed PY - 2005/6/25/medline PY - 2004/12/1/entrez SP - 1606 EP - 12 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 25 IS - 12 N2 - AIM: To investigate the effect of curcumin on tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in rat cortical neurons and to explore the possible mechanism. METHODS: Primary cultured rat cortical neurons were performed in vitro and cell viability was measured by MTT assay. DNA fragmentation was used to evaluate cell apoptosis. Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (Deltapsim) was determined by flow cytometric assay. Cellular glutathione (GSH) content was measured by spectrophotometer. Bcl-2 family proteins, cytochrome c, cleaved caspase-3, and poly (ADP-ribose) polymerase (PARP) were detected by Western blot. RESULTS: Exposure of tBHP 100 micromol/L to neurons for 60 min resulted in DYm loss and cytochrome c release from mitochondria and subsequent activation of caspase-3 and PARP cleavation, and cell apoptosis. After removal of tBHP and then further treatment with curcumin (2.5-20 micromol/L) for 18 h, curcumin abrogated Deltapsim loss and cytochrome c release, blocked activation of caspase 3, and altered the expression of Bcl-2 family. Further curcumin treatment also prevented cellular GSH and decreased intracellular ROS generation markedly. Curcumin eventually attenuated tBHP-induced apoptosis in cortical neurons. CONCLUSION: Curcumin may attenuate oxidative damages in cortical neurons by reducing intracellular production of ROS and protecting mitochondria from oxidative damage. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/15569404/Curcumin_protects_mitochondria_from_oxidative_damage_and_attenuates_apoptosis_in_cortical_neurons_ L2 - http://www.chinaphar.com/1671-4083/25/1606.pdf DB - PRIME DP - Unbound Medicine ER -