Picogram per liter level determination of estrogens in natural waters and waterworks by a fully automated on-line solid-phase extraction-liquid chromatography-electrospray tandem mass spectrometry method.
Anal Chem. 2004 Dec 01; 76(23):6998-7006.AC

Abstract

The present work describes a novel, fully automated method, based on on-line solid-phase extraction-liquid chromatography-electrospray tandem mass spectrometry (SPE-LC-ESI-MS-MS), which allows the unequivocal identification and quantification of the most environmentally relevant estrogens (estradiol, estrone, estriol, estradiol-17-glucuronide, estradiol-17-acetate, estrone-3-sulfate, ethynyl estradiol, diethylstilbestrol) in natural and treated waters at levels well below those of concern (limits of quantification between 0.02 and 1.02 ng/L). The method is highly precise, with relative standard deviations varying between 1.43 and 3.89%, and accurate (recovery percentages >74 %). This method was used to track the presence and fate of the target compounds in a waterworks and to evaluate the removal efficiency of the treatment processes applied. Only estrone and estrone-3-sulfate were detected in the river water used as source (at 0.68 and 0.33 ng/L, respectively). After progressive removal through the various treatment steps, none of them were detected in the finished drinking water. In addition to selectivity, sensitivity, repeatability, and automation (up to 15 samples plus 6 calibration solutions and 1 blank can be analyzed unattended), this technique offers fairly high throughput (analysis time per sample is 60 min), low time and solvent consumption, and ease of use.

Authors+Show Affiliations

Rodriguez-Mozaz S

Department of Environmental Chemistry, IIQAB-CSIC, C/Jordi Girona Salgado 18-26, 08034 Barcelona, Spain.

Lopez de Alda MJ

No affiliation info available

Barceló D

No affiliation info available

MeSH

AutoanalysisChromatography, LiquidEstrogensHydrogen-Ion ConcentrationQuality ControlSensitivity and SpecificitySpectrometry, Mass, Electrospray IonizationWaterWater PurificationWater Supply

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15571352

Citation

Rodriguez-Mozaz, Sara, et al. "Picogram Per Liter Level Determination of Estrogens in Natural Waters and Waterworks By a Fully Automated On-line Solid-phase Extraction-liquid Chromatography-electrospray Tandem Mass Spectrometry Method." Analytical Chemistry, vol. 76, no. 23, 2004, pp. 6998-7006.

Rodriguez-Mozaz S, Lopez de Alda MJ, Barceló D. Picogram per liter level determination of estrogens in natural waters and waterworks by a fully automated on-line solid-phase extraction-liquid chromatography-electrospray tandem mass spectrometry method. Anal Chem. 2004;76(23):6998-7006.

Rodriguez-Mozaz, S., Lopez de Alda, M. J., & Barceló, D. (2004). Picogram per liter level determination of estrogens in natural waters and waterworks by a fully automated on-line solid-phase extraction-liquid chromatography-electrospray tandem mass spectrometry method. Analytical Chemistry, 76(23), 6998-7006.

Rodriguez-Mozaz S, Lopez de Alda MJ, Barceló D. Picogram Per Liter Level Determination of Estrogens in Natural Waters and Waterworks By a Fully Automated On-line Solid-phase Extraction-liquid Chromatography-electrospray Tandem Mass Spectrometry Method. Anal Chem. 2004 Dec 1;76(23):6998-7006. PubMed PMID: 15571352.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Picogram per liter level determination of estrogens in natural waters and waterworks by a fully automated on-line solid-phase extraction-liquid chromatography-electrospray tandem mass spectrometry method. AU - Rodriguez-Mozaz,Sara, AU - Lopez de Alda,Maria J, AU - Barceló,Damià, PY - 2004/12/2/pubmed PY - 2006/7/27/medline PY - 2004/12/2/entrez SP - 6998 EP - 7006 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 23 N2 - The present work describes a novel, fully automated method, based on on-line solid-phase extraction-liquid chromatography-electrospray tandem mass spectrometry (SPE-LC-ESI-MS-MS), which allows the unequivocal identification and quantification of the most environmentally relevant estrogens (estradiol, estrone, estriol, estradiol-17-glucuronide, estradiol-17-acetate, estrone-3-sulfate, ethynyl estradiol, diethylstilbestrol) in natural and treated waters at levels well below those of concern (limits of quantification between 0.02 and 1.02 ng/L). The method is highly precise, with relative standard deviations varying between 1.43 and 3.89%, and accurate (recovery percentages >74 %). This method was used to track the presence and fate of the target compounds in a waterworks and to evaluate the removal efficiency of the treatment processes applied. Only estrone and estrone-3-sulfate were detected in the river water used as source (at 0.68 and 0.33 ng/L, respectively). After progressive removal through the various treatment steps, none of them were detected in the finished drinking water. In addition to selectivity, sensitivity, repeatability, and automation (up to 15 samples plus 6 calibration solutions and 1 blank can be analyzed unattended), this technique offers fairly high throughput (analysis time per sample is 60 min), low time and solvent consumption, and ease of use. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15571352/Picogram_per_liter_level_determination_of_estrogens_in_natural_waters_and_waterworks_by_a_fully_automated_on_line_solid_phase_extraction_liquid_chromatography_electrospray_tandem_mass_spectrometry_method_ DB - PRIME DP - Unbound Medicine ER -

Tags

Picogram per liter level determination of estrogens in natural waters and waterworks by a fully automated on-line solid-phase extraction-liquid chromatography-electrospray tandem mass spectrometry method.Anal Chem. 2004 Dec 01; 76(23):6998-7006.AC