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Quantitation of lysergic acid diethylamide in urine using atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry.
Anal Chem. 2004 Dec 01; 76(23):7143-8.AC

Abstract

A quantitative method was developed for analysis of lysergic acid diethylamide (LSD) in urine using atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry (AP MALDI-ITMS). Following solid-phase extraction of LSD from urine samples, extracts were analyzed by AP MALDI-ITMS. The identity of LSD was confirmed by fragmentation of the [M + H](+) ion using tandem mass spectrometry. The quantification of LSD was achieved using stable-isotope-labeled LSD (LSD-d(3)) as the internal standard. The [M + H](+) ion fragmented to produce a dominant fragment ion, which was used for a selected reaction monitoring (SRM) method for quantitative analysis of LSD. SRM was compared with selected ion monitoring and produced a wider linear range and lower limit of quantification. For SRM analysis of samples of LSD spiked in urine, the calibration curve was linear in the range of 1-100 ng/mL with a coefficient of determination, r(2), of 0.9917. This assay was used to determine LSD in urine samples and the AP MALDI-MS results were comparable to the HPLC/ ESI-MS results.

Authors+Show Affiliations

Institute for National Measurement Standards, National Research Council of Canada, Ottawa K1A 0R6, Canada.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15571372

Citation

Cui, Meng, et al. "Quantitation of Lysergic Acid Diethylamide in Urine Using Atmospheric Pressure Matrix-assisted Laser Desorption/ionization Ion Trap Mass Spectrometry." Analytical Chemistry, vol. 76, no. 23, 2004, pp. 7143-8.
Cui M, McCooeye MA, Fraser C, et al. Quantitation of lysergic acid diethylamide in urine using atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry. Anal Chem. 2004;76(23):7143-8.
Cui, M., McCooeye, M. A., Fraser, C., & Mester, Z. (2004). Quantitation of lysergic acid diethylamide in urine using atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry. Analytical Chemistry, 76(23), 7143-8.
Cui M, et al. Quantitation of Lysergic Acid Diethylamide in Urine Using Atmospheric Pressure Matrix-assisted Laser Desorption/ionization Ion Trap Mass Spectrometry. Anal Chem. 2004 Dec 1;76(23):7143-8. PubMed PMID: 15571372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitation of lysergic acid diethylamide in urine using atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry. AU - Cui,Meng, AU - McCooeye,Margaret A, AU - Fraser,Catharine, AU - Mester,Zoltán, PY - 2004/12/2/pubmed PY - 2006/7/27/medline PY - 2004/12/2/entrez SP - 7143 EP - 8 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 23 N2 - A quantitative method was developed for analysis of lysergic acid diethylamide (LSD) in urine using atmospheric pressure matrix-assisted laser desorption/ionization ion trap mass spectrometry (AP MALDI-ITMS). Following solid-phase extraction of LSD from urine samples, extracts were analyzed by AP MALDI-ITMS. The identity of LSD was confirmed by fragmentation of the [M + H](+) ion using tandem mass spectrometry. The quantification of LSD was achieved using stable-isotope-labeled LSD (LSD-d(3)) as the internal standard. The [M + H](+) ion fragmented to produce a dominant fragment ion, which was used for a selected reaction monitoring (SRM) method for quantitative analysis of LSD. SRM was compared with selected ion monitoring and produced a wider linear range and lower limit of quantification. For SRM analysis of samples of LSD spiked in urine, the calibration curve was linear in the range of 1-100 ng/mL with a coefficient of determination, r(2), of 0.9917. This assay was used to determine LSD in urine samples and the AP MALDI-MS results were comparable to the HPLC/ ESI-MS results. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15571372/Quantitation_of_lysergic_acid_diethylamide_in_urine_using_atmospheric_pressure_matrix_assisted_laser_desorption/ionization_ion_trap_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -