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Flow cytometric evaluation of bone marrow plasma cells using CD19, CD45, CD56, CD38, and CD138 and correlation with bone marrow infiltration ratio in multiple myeloma patients.
Saudi Med J. 2004 Nov; 25(11):1587-92.SM

Abstract

OBJECTIVE

To examine the co-expression of CD19, CD45, CD38, CD56, and CD138 molecules in plasma cells of bone marrow (BM) aspirates and their relation with BM infiltration, and treatment in patients with multiple myeloma by flow cytometry.

METHODS

Forty BM aspirate samples were assessed from 40 patients at diagnosis and on follow-up at the Medical Oncology Department, Cukurova University, Balcali Hospital, Turkey, between 2002 and 2004. The mean age was 56.83 +/- 9.1 and male:female ratio was 2.6. All patients received at least 4 courses of VAD(vincristine, adriamycin, dexamethasone) regimens and 20 of them were also treated with high dose melphalan and peripheral autologous stem cell transplantation. The median follow-up period was 19.1 +/- 22.7 months.

RESULTS

Using light microscopy the BM smears stained with hematoxylin and eosin from patients on follow up were classified into one of 3 categories, complete remission (CR) (<5%), partial remission (PR) (>5% and <30%), and extensive infiltration (EI) (>30%). According to infiltration ratio 23 were evaluated CR, 2 were PR and 15 were EI. The mean value of CD19 was 6.01 +/- 9.5%, CD56 = 9.9 +/- 6.8%, CD138 = 8.6 +/- 5.6%, CD45 = 84.2 +/- 22.3% and CD38 = 59.5 +/- 25.4%. The flow cytometric analyses revealed that only the mean value of CD38 and CD45 expression were significantly high. We correlated infiltration ratio with each parametric and found statistically significant relations. We also correlated independent variables with each other and found a relation between CD38 and CD19 (p=0.005). We also defined the groups whether treated with peripheral autologous transplantation or not and compared the independent variables between them, in which CD138 was statistically significant (p=0.02).

CONCLUSION

We suggest BM plasma cells expressed mainly by CD38 and CD45 may have a role in generation of BM plasma cells and that CD138 expression may be considered in follow-up for minimal residual disease after autologous transplantation in myeloma patients.

Authors+Show Affiliations

Department of Medical Oncology, Cukurova University, Faculty of Medicine, Balcali Hospital, Yuregir 01330, Adana, Turkey. iokara@cu.edu.trNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15573184

Citation

Kara, Ismail O., et al. "Flow Cytometric Evaluation of Bone Marrow Plasma Cells Using CD19, CD45, CD56, CD38, and CD138 and Correlation With Bone Marrow Infiltration Ratio in Multiple Myeloma Patients." Saudi Medical Journal, vol. 25, no. 11, 2004, pp. 1587-92.
Kara IO, Sahin B, Paydas S, et al. Flow cytometric evaluation of bone marrow plasma cells using CD19, CD45, CD56, CD38, and CD138 and correlation with bone marrow infiltration ratio in multiple myeloma patients. Saudi Med J. 2004;25(11):1587-92.
Kara, I. O., Sahin, B., Paydas, S., & Cetiner, S. (2004). Flow cytometric evaluation of bone marrow plasma cells using CD19, CD45, CD56, CD38, and CD138 and correlation with bone marrow infiltration ratio in multiple myeloma patients. Saudi Medical Journal, 25(11), 1587-92.
Kara IO, et al. Flow Cytometric Evaluation of Bone Marrow Plasma Cells Using CD19, CD45, CD56, CD38, and CD138 and Correlation With Bone Marrow Infiltration Ratio in Multiple Myeloma Patients. Saudi Med J. 2004;25(11):1587-92. PubMed PMID: 15573184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flow cytometric evaluation of bone marrow plasma cells using CD19, CD45, CD56, CD38, and CD138 and correlation with bone marrow infiltration ratio in multiple myeloma patients. AU - Kara,Ismail O, AU - Sahin,Berksoy, AU - Paydas,Semra, AU - Cetiner,Salih, PY - 2004/12/2/pubmed PY - 2005/2/3/medline PY - 2004/12/2/entrez SP - 1587 EP - 92 JF - Saudi medical journal JO - Saudi Med J VL - 25 IS - 11 N2 - OBJECTIVE: To examine the co-expression of CD19, CD45, CD38, CD56, and CD138 molecules in plasma cells of bone marrow (BM) aspirates and their relation with BM infiltration, and treatment in patients with multiple myeloma by flow cytometry. METHODS: Forty BM aspirate samples were assessed from 40 patients at diagnosis and on follow-up at the Medical Oncology Department, Cukurova University, Balcali Hospital, Turkey, between 2002 and 2004. The mean age was 56.83 +/- 9.1 and male:female ratio was 2.6. All patients received at least 4 courses of VAD(vincristine, adriamycin, dexamethasone) regimens and 20 of them were also treated with high dose melphalan and peripheral autologous stem cell transplantation. The median follow-up period was 19.1 +/- 22.7 months. RESULTS: Using light microscopy the BM smears stained with hematoxylin and eosin from patients on follow up were classified into one of 3 categories, complete remission (CR) (<5%), partial remission (PR) (>5% and <30%), and extensive infiltration (EI) (>30%). According to infiltration ratio 23 were evaluated CR, 2 were PR and 15 were EI. The mean value of CD19 was 6.01 +/- 9.5%, CD56 = 9.9 +/- 6.8%, CD138 = 8.6 +/- 5.6%, CD45 = 84.2 +/- 22.3% and CD38 = 59.5 +/- 25.4%. The flow cytometric analyses revealed that only the mean value of CD38 and CD45 expression were significantly high. We correlated infiltration ratio with each parametric and found statistically significant relations. We also correlated independent variables with each other and found a relation between CD38 and CD19 (p=0.005). We also defined the groups whether treated with peripheral autologous transplantation or not and compared the independent variables between them, in which CD138 was statistically significant (p=0.02). CONCLUSION: We suggest BM plasma cells expressed mainly by CD38 and CD45 may have a role in generation of BM plasma cells and that CD138 expression may be considered in follow-up for minimal residual disease after autologous transplantation in myeloma patients. SN - 0379-5284 UR - https://www.unboundmedicine.com/medline/citation/15573184/Flow_cytometric_evaluation_of_bone_marrow_plasma_cells_using_CD19_CD45_CD56_CD38_and_CD138_and_correlation_with_bone_marrow_infiltration_ratio_in_multiple_myeloma_patients_ L2 - http://www.diseaseinfosearch.org/result/4962 DB - PRIME DP - Unbound Medicine ER -