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Analysis of gamma-hydroxybutyric acid, DL-lactic acid, glycolic acid, ethylene glycol and other glycols in body fluids by a direct injection gas chromatography-mass spectrometry assay for wide use.
Clin Chem Lab Med. 2004; 42(11):1341-5.CC

Abstract

Analysis of blood of severely intoxicated patients always requires prompt investigation. Diagnosis of intoxication with ethylene glycol, gamma-hydroxybutyric acid or D-lactic acid takes hours, since several different procedures are required. Rapid derivatization of the common hydroxyl function may resolve this analytical problem. Here we describe a fast method for the simultaneous measurement of ethylene glycol, glycolic acid, gamma-hydroxybutyric acid and racemic lactic acid. Only 20 microl of serum, plasma or urine are required for immediate derivatization at 70 degrees C with 750 microl of bis-N,O-trimethylsilyl trifluoroacetamide after adding 20 microl of internal standard solution (1,3-propylene glycol) and 20 microl of the catalyst dimethylformamide. After centrifugation an aliquot is transferred to a gas chromatographic system and analyzed with electron-impact mass spectrometry in selective ion monitoring mode. The derivatized acids and ethylene glycol are well separated and detected with a limit of detection ranging from 0.12 mg/l for ethylene glycol to 0.95 mg/l for gamma-hydroxybutyric acid, while the limit of quantification ranged from 0.4 mg/l for ethylene glycol to 3.15 mg/l for gamma-hydroxybutyric acid. The method is linear from 0.5 to 1800 mg/l blood for ethylene glycol, from 0.7 to 1200 mg/l for lactic acid, from 1.2 to 1800 mg/l for glycolic acid, and from 3.2 to 200 mg/l for gamma-hydroxybutyric acid, with analytical recoveries, accuracy, day-to-day and within-day precision well within the required limits. Total analysis time with one calibrator was 30 min, derivatization time included. This method is very suitable for emergency toxicology, since several toxic substances can be quantified simultaneously in a fast and sensitive manner.

Authors+Show Affiliations

Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15576294

Citation

Van Hee, Paul, et al. "Analysis of Gamma-hydroxybutyric Acid, DL-lactic Acid, Glycolic Acid, Ethylene Glycol and Other Glycols in Body Fluids By a Direct Injection Gas Chromatography-mass Spectrometry Assay for Wide Use." Clinical Chemistry and Laboratory Medicine, vol. 42, no. 11, 2004, pp. 1341-5.
Van Hee P, Neels H, De Doncker M, et al. Analysis of gamma-hydroxybutyric acid, DL-lactic acid, glycolic acid, ethylene glycol and other glycols in body fluids by a direct injection gas chromatography-mass spectrometry assay for wide use. Clin Chem Lab Med. 2004;42(11):1341-5.
Van Hee, P., Neels, H., De Doncker, M., Vrydags, N., Schatteman, K., Uyttenbroeck, W., Hamers, N., Himpe, D., & Lambert, W. (2004). Analysis of gamma-hydroxybutyric acid, DL-lactic acid, glycolic acid, ethylene glycol and other glycols in body fluids by a direct injection gas chromatography-mass spectrometry assay for wide use. Clinical Chemistry and Laboratory Medicine, 42(11), 1341-5.
Van Hee P, et al. Analysis of Gamma-hydroxybutyric Acid, DL-lactic Acid, Glycolic Acid, Ethylene Glycol and Other Glycols in Body Fluids By a Direct Injection Gas Chromatography-mass Spectrometry Assay for Wide Use. Clin Chem Lab Med. 2004;42(11):1341-5. PubMed PMID: 15576294.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of gamma-hydroxybutyric acid, DL-lactic acid, glycolic acid, ethylene glycol and other glycols in body fluids by a direct injection gas chromatography-mass spectrometry assay for wide use. AU - Van Hee,Paul, AU - Neels,Hugo, AU - De Doncker,Mireille, AU - Vrydags,Nicolas, AU - Schatteman,Katinka, AU - Uyttenbroeck,Wim, AU - Hamers,Nicole, AU - Himpe,Dirk, AU - Lambert,Willy, PY - 2004/12/4/pubmed PY - 2005/4/19/medline PY - 2004/12/4/entrez SP - 1341 EP - 5 JF - Clinical chemistry and laboratory medicine JO - Clin Chem Lab Med VL - 42 IS - 11 N2 - Analysis of blood of severely intoxicated patients always requires prompt investigation. Diagnosis of intoxication with ethylene glycol, gamma-hydroxybutyric acid or D-lactic acid takes hours, since several different procedures are required. Rapid derivatization of the common hydroxyl function may resolve this analytical problem. Here we describe a fast method for the simultaneous measurement of ethylene glycol, glycolic acid, gamma-hydroxybutyric acid and racemic lactic acid. Only 20 microl of serum, plasma or urine are required for immediate derivatization at 70 degrees C with 750 microl of bis-N,O-trimethylsilyl trifluoroacetamide after adding 20 microl of internal standard solution (1,3-propylene glycol) and 20 microl of the catalyst dimethylformamide. After centrifugation an aliquot is transferred to a gas chromatographic system and analyzed with electron-impact mass spectrometry in selective ion monitoring mode. The derivatized acids and ethylene glycol are well separated and detected with a limit of detection ranging from 0.12 mg/l for ethylene glycol to 0.95 mg/l for gamma-hydroxybutyric acid, while the limit of quantification ranged from 0.4 mg/l for ethylene glycol to 3.15 mg/l for gamma-hydroxybutyric acid. The method is linear from 0.5 to 1800 mg/l blood for ethylene glycol, from 0.7 to 1200 mg/l for lactic acid, from 1.2 to 1800 mg/l for glycolic acid, and from 3.2 to 200 mg/l for gamma-hydroxybutyric acid, with analytical recoveries, accuracy, day-to-day and within-day precision well within the required limits. Total analysis time with one calibrator was 30 min, derivatization time included. This method is very suitable for emergency toxicology, since several toxic substances can be quantified simultaneously in a fast and sensitive manner. SN - 1434-6621 UR - https://www.unboundmedicine.com/medline/citation/15576294/Analysis_of_gamma_hydroxybutyric_acid_DL_lactic_acid_glycolic_acid_ethylene_glycol_and_other_glycols_in_body_fluids_by_a_direct_injection_gas_chromatography_mass_spectrometry_assay_for_wide_use_ DB - PRIME DP - Unbound Medicine ER -