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Activity of gut proteinases from Cry1Ab-selected colonies of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae).
Pest Manag Sci. 2004 Dec; 60(12):1189-96.PM

Abstract

Susceptibility to the Cry1Ab protoxin and toxin from Bacillus thuringiensis (Berliner) and activity of gut proteinases were assessed in both susceptible and Cry1Ab-selected colonies of European corn borer, Ostrinia nubilalis (Hubner). Resistance in two different selected colonies was at least 6- and 15-fold for the Cry1Ab protoxin and 108- and 484-fold for the Cry1Ab toxin. Activities of trypsin-like, chymotrypsin-like and elastase-like proteinases were variable among the colonies tested and not indicative of a major contribution to Cry1Ab resistance. Activation of the 130-kDa Cry1Ab protoxin occurred rapidly in all colonies, with no apparent differences among colonies. In addition, there were no apparent changes in activated Cry1Ab processing, indicating that proteolytic degradation was not associated with resistance. These results suggest that mechanisms other than proteolytic activation of protoxin and toxin degradation, such as target site modification may be involved in the resistance to B thuringiensis Cry1Ab in these O nubilalis colonies.

Authors+Show Affiliations

Siqueira HA
Department of Entomology, 202 Plant Industry Bldg, University of Nebraska-Lincoln, NE 68583, USA.
Nickerson KW
No affiliation info available
Moellenbeck D
No affiliation info available
Siegfried BD
No affiliation info available

MeSH

AnimalsBacillus thuringiensis ToxinsBacterial ProteinsBacterial ToxinsEndotoxinsGastrointestinal TractHemolysin ProteinsInsecticide ResistanceMothsPeptide HydrolasesPest Control, Biological

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15578599

Citation

Siqueira, Herbert A A., et al. "Activity of Gut Proteinases From Cry1Ab-selected Colonies of the European Corn Borer, Ostrinia Nubilalis (Lepidoptera: Crambidae)." Pest Management Science, vol. 60, no. 12, 2004, pp. 1189-96.
Siqueira HA, Nickerson KW, Moellenbeck D, et al. Activity of gut proteinases from Cry1Ab-selected colonies of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). Pest Manag Sci. 2004;60(12):1189-96.
Siqueira, H. A., Nickerson, K. W., Moellenbeck, D., & Siegfried, B. D. (2004). Activity of gut proteinases from Cry1Ab-selected colonies of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). Pest Management Science, 60(12), 1189-96.
Siqueira HA, et al. Activity of Gut Proteinases From Cry1Ab-selected Colonies of the European Corn Borer, Ostrinia Nubilalis (Lepidoptera: Crambidae). Pest Manag Sci. 2004;60(12):1189-96. PubMed PMID: 15578599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activity of gut proteinases from Cry1Ab-selected colonies of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). AU - Siqueira,Herbert A A, AU - Nickerson,Kenneth W, AU - Moellenbeck,Daniel, AU - Siegfried,Blair D, PY - 2004/12/4/pubmed PY - 2005/2/16/medline PY - 2004/12/4/entrez SP - 1189 EP - 96 JF - Pest management science JO - Pest Manag Sci VL - 60 IS - 12 N2 - Susceptibility to the Cry1Ab protoxin and toxin from Bacillus thuringiensis (Berliner) and activity of gut proteinases were assessed in both susceptible and Cry1Ab-selected colonies of European corn borer, Ostrinia nubilalis (Hubner). Resistance in two different selected colonies was at least 6- and 15-fold for the Cry1Ab protoxin and 108- and 484-fold for the Cry1Ab toxin. Activities of trypsin-like, chymotrypsin-like and elastase-like proteinases were variable among the colonies tested and not indicative of a major contribution to Cry1Ab resistance. Activation of the 130-kDa Cry1Ab protoxin occurred rapidly in all colonies, with no apparent differences among colonies. In addition, there were no apparent changes in activated Cry1Ab processing, indicating that proteolytic degradation was not associated with resistance. These results suggest that mechanisms other than proteolytic activation of protoxin and toxin degradation, such as target site modification may be involved in the resistance to B thuringiensis Cry1Ab in these O nubilalis colonies. SN - 1526-498X UR - https://www.unboundmedicine.com/medline/citation/15578599/Activity_of_gut_proteinases_from_Cry1Ab_selected_colonies_of_the_European_corn_borer_Ostrinia_nubilalis__Lepidoptera:_Crambidae__ DB - PRIME DP - Unbound Medicine ER -