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mRNA analysis of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis using a real-time quantitative RT-PCR assay.
Hum Reprod. 2005 Jan; 20(1):272-8.HR

Abstract

BACKGROUND

The plasminogen activator (PA) and matrix metalloproteinase (MMP) systems are implicated in the establishment of endometriosis. The mechanisms by which these systems are involved in the pathogenesis of this disease are not well defined and controversial results have been published. The aim of this study was to analyse mRNA and protein levels of several components of the PA and MMP systems in endometriotic tissue and endometrium from women with and without endometriosis.

METHODS AND RESULTS

Real-time quantitative RT-PCR assays were developed to quantify mRNA levels of these components in 57 women with endometriosis and 32 controls. Endometrium of women with endometriosis showed higher mRNA and antigenic levels of urokinase type-PA (uPA) and MMP-3 than endometrium from controls. In these patients, ovarian endometriotic tissue had higher mRNA and antigenic levels of PA inhibitor type 1 (PAI-1) and MMP inhibitor type 1 (TIMP-1) than endometrium.

CONCLUSIONS

The increase in mRNA and protein levels of uPA and MMP-3 observed in endometrium of women with endometriosis may facilitate the attachment of endometrial tissue to the peritoneum and ovarian surface, as well as the invasion of the extracellular matrix. This process would lead to the formation of early endometriotic lesions. Once the ovarian endometriotic cyst is developed, PAI-1 and TIMP-1 would increase which could explain the frequent clinical finding of an endometrioma without invasion of the adjacent ovarian tissue.

Authors+Show Affiliations

Research Center, Hospital Universitario La Fe, Valencia, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15579491

Citation

Ramón, L, et al. "MRNA Analysis of Several Components of the Plasminogen Activator and Matrix Metalloproteinase Systems in Endometriosis Using a Real-time Quantitative RT-PCR Assay." Human Reproduction (Oxford, England), vol. 20, no. 1, 2005, pp. 272-8.
Ramón L, Gilabert-Estellés J, Castelló R, et al. MRNA analysis of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis using a real-time quantitative RT-PCR assay. Hum Reprod. 2005;20(1):272-8.
Ramón, L., Gilabert-Estellés, J., Castelló, R., Gilabert, J., España, F., Romeu, A., Chirivella, M., Aznar, J., & Estellés, A. (2005). MRNA analysis of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis using a real-time quantitative RT-PCR assay. Human Reproduction (Oxford, England), 20(1), 272-8.
Ramón L, et al. MRNA Analysis of Several Components of the Plasminogen Activator and Matrix Metalloproteinase Systems in Endometriosis Using a Real-time Quantitative RT-PCR Assay. Hum Reprod. 2005;20(1):272-8. PubMed PMID: 15579491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - mRNA analysis of several components of the plasminogen activator and matrix metalloproteinase systems in endometriosis using a real-time quantitative RT-PCR assay. AU - Ramón,L, AU - Gilabert-Estellés,J, AU - Castelló,R, AU - Gilabert,J, AU - España,F, AU - Romeu,A, AU - Chirivella,M, AU - Aznar,J, AU - Estellés,A, Y1 - 2004/12/03/ PY - 2004/12/8/pubmed PY - 2005/6/14/medline PY - 2004/12/8/entrez SP - 272 EP - 8 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 20 IS - 1 N2 - BACKGROUND: The plasminogen activator (PA) and matrix metalloproteinase (MMP) systems are implicated in the establishment of endometriosis. The mechanisms by which these systems are involved in the pathogenesis of this disease are not well defined and controversial results have been published. The aim of this study was to analyse mRNA and protein levels of several components of the PA and MMP systems in endometriotic tissue and endometrium from women with and without endometriosis. METHODS AND RESULTS: Real-time quantitative RT-PCR assays were developed to quantify mRNA levels of these components in 57 women with endometriosis and 32 controls. Endometrium of women with endometriosis showed higher mRNA and antigenic levels of urokinase type-PA (uPA) and MMP-3 than endometrium from controls. In these patients, ovarian endometriotic tissue had higher mRNA and antigenic levels of PA inhibitor type 1 (PAI-1) and MMP inhibitor type 1 (TIMP-1) than endometrium. CONCLUSIONS: The increase in mRNA and protein levels of uPA and MMP-3 observed in endometrium of women with endometriosis may facilitate the attachment of endometrial tissue to the peritoneum and ovarian surface, as well as the invasion of the extracellular matrix. This process would lead to the formation of early endometriotic lesions. Once the ovarian endometriotic cyst is developed, PAI-1 and TIMP-1 would increase which could explain the frequent clinical finding of an endometrioma without invasion of the adjacent ovarian tissue. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/15579491/mRNA_analysis_of_several_components_of_the_plasminogen_activator_and_matrix_metalloproteinase_systems_in_endometriosis_using_a_real_time_quantitative_RT_PCR_assay_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/deh571 DB - PRIME DP - Unbound Medicine ER -