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A new approach for the assessment of acrylamide toxicity using a green paramecium.
Toxicol In Vitro. 2005 Feb; 19(1):99-105.TV

Abstract

Exposure to acrylamide induces neurotoxic effects in humans. In addition, it induces genotoxic, reproductive and carcinogenic effects in laboratory animals. However, no convenient bioassay system for assessing acrylamide toxicity to animal and plant cells has been proposed to date. The present study aims to evaluate acrylamide toxicity to a green paramecium, Paramecium bursaria, bearing many endosymbiotic algae, because some chemicals are highly toxic to paramecia or microalgae, and some protozoa are already used for evaluation of environmental contaminations. Results showed that high acrylamide concentrations (> or = 1500 mg/l) have a lethal effect on P. bursaria. Although low acrylamide concentrations (< or = 150 mg/l) induced less change on the paramecium growth, the number of endosymbiotically growing algal cells drastically decreased. The acrylamide concentration required to induce a 50% decrease in the cell number (IC(50)) was determined to be 7.8 mg/l for endosymbiotic algae, indicating that the algal sensitivity to acrylamide was 7 and 15 times higher than that of Syrian hamster embryo (SHE) cells and the host cells, respectively. Here, we propose the use of P. bursaria being a convenient and sensitive bioindicator as a new approach for the assessment of acrylamide toxicity.

Authors+Show Affiliations

Takahashi T
Faculty of Science, Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.
Yoshii M
No affiliation info available
Kawano T
No affiliation info available
Kosaka T
No affiliation info available
Hosoya H
No affiliation info available

MeSH

AcrylamideAnimal Testing AlternativesAnimalsCell CountChlorophyllCricetinaeDNA, AlgalDose-Response Relationship, DrugEukaryotaInhibitory Concentration 50MesocricetusParameciumPolymerase Chain ReactionSymbiosisToxicity Tests

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15582361

Citation

Takahashi, Toshiyuki, et al. "A New Approach for the Assessment of Acrylamide Toxicity Using a Green Paramecium." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 19, no. 1, 2005, pp. 99-105.
Takahashi T, Yoshii M, Kawano T, et al. A new approach for the assessment of acrylamide toxicity using a green paramecium. Toxicol In Vitro. 2005;19(1):99-105.
Takahashi, T., Yoshii, M., Kawano, T., Kosaka, T., & Hosoya, H. (2005). A new approach for the assessment of acrylamide toxicity using a green paramecium. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 19(1), 99-105.
Takahashi T, et al. A New Approach for the Assessment of Acrylamide Toxicity Using a Green Paramecium. Toxicol In Vitro. 2005;19(1):99-105. PubMed PMID: 15582361.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new approach for the assessment of acrylamide toxicity using a green paramecium. AU - Takahashi,Toshiyuki, AU - Yoshii,Masanobu, AU - Kawano,Tomonori, AU - Kosaka,Toshikazu, AU - Hosoya,Hiroshi, PY - 2004/01/20/received PY - 2004/06/10/revised PY - 2004/06/25/accepted PY - 2004/12/8/pubmed PY - 2005/5/27/medline PY - 2004/12/8/entrez SP - 99 EP - 105 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 19 IS - 1 N2 - Exposure to acrylamide induces neurotoxic effects in humans. In addition, it induces genotoxic, reproductive and carcinogenic effects in laboratory animals. However, no convenient bioassay system for assessing acrylamide toxicity to animal and plant cells has been proposed to date. The present study aims to evaluate acrylamide toxicity to a green paramecium, Paramecium bursaria, bearing many endosymbiotic algae, because some chemicals are highly toxic to paramecia or microalgae, and some protozoa are already used for evaluation of environmental contaminations. Results showed that high acrylamide concentrations (> or = 1500 mg/l) have a lethal effect on P. bursaria. Although low acrylamide concentrations (< or = 150 mg/l) induced less change on the paramecium growth, the number of endosymbiotically growing algal cells drastically decreased. The acrylamide concentration required to induce a 50% decrease in the cell number (IC(50)) was determined to be 7.8 mg/l for endosymbiotic algae, indicating that the algal sensitivity to acrylamide was 7 and 15 times higher than that of Syrian hamster embryo (SHE) cells and the host cells, respectively. Here, we propose the use of P. bursaria being a convenient and sensitive bioindicator as a new approach for the assessment of acrylamide toxicity. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/15582361/A_new_approach_for_the_assessment_of_acrylamide_toxicity_using_a_green_paramecium_ DB - PRIME DP - Unbound Medicine ER -