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Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes.
J Clin Microbiol. 2004 Dec; 42(12):5871-4.JC

Abstract

Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.

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Authors+Show Affiliations

Thibault FM
Centre de Recherches du Service de Santé des Armées, 24 Avenue des maquis du Grésivaudan, B.P. 87, F-38702 La Tronche, France. fthibault@crssa.net
Valade E
No affiliation info available
Vidal DR
No affiliation info available

MeSH

AnimalsBacterial ProteinsBacterial Typing TechniquesBurkholderiaBurkholderia malleiBurkholderia pseudomalleiDNA ProbesGlandersHorse DiseasesHorsesHumansHydrolysisMelioidosisPolymerase Chain ReactionSpecies Specificity

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15583328

Citation

Thibault, F M., et al. "Identification and Discrimination of Burkholderia Pseudomallei, B. Mallei, and B. Thailandensis By Real-time PCR Targeting Type III Secretion System Genes." Journal of Clinical Microbiology, vol. 42, no. 12, 2004, pp. 5871-4.
Thibault FM, Valade E, Vidal DR. Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes. J Clin Microbiol. 2004;42(12):5871-4.
Thibault, F. M., Valade, E., & Vidal, D. R. (2004). Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes. Journal of Clinical Microbiology, 42(12), 5871-4.
Thibault FM, Valade E, Vidal DR. Identification and Discrimination of Burkholderia Pseudomallei, B. Mallei, and B. Thailandensis By Real-time PCR Targeting Type III Secretion System Genes. J Clin Microbiol. 2004;42(12):5871-4. PubMed PMID: 15583328.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by real-time PCR targeting type III secretion system genes. AU - Thibault,F M, AU - Valade,E, AU - Vidal,D R, PY - 2004/12/8/pubmed PY - 2005/2/3/medline PY - 2004/12/8/entrez SP - 5871 EP - 4 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 42 IS - 12 N2 - Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/15583328/Identification_and_discrimination_of_Burkholderia_pseudomallei_B__mallei_and_B__thailandensis_by_real_time_PCR_targeting_type_III_secretion_system_genes_ DB - PRIME DP - Unbound Medicine ER -