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False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide.
J Clin Microbiol. 2004 Dec; 42(12):5885-8.JC

Abstract

Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.

Authors+Show Affiliations

Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong. hkumicro@hkucc.hku.hk.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15583332

Citation

Woo, Patrick C Y., et al. "False-positive Results in a Recombinant Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV) Nucleocapsid Enzyme-linked Immunosorbent Assay Due to HCoV-OC43 and HCoV-229E Rectified By Western Blotting With Recombinant SARS-CoV Spike Polypeptide." Journal of Clinical Microbiology, vol. 42, no. 12, 2004, pp. 5885-8.
Woo PC, Lau SK, Wong BH, et al. False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide. J Clin Microbiol. 2004;42(12):5885-8.
Woo, P. C., Lau, S. K., Wong, B. H., Chan, K. H., Hui, W. T., Kwan, G. S., Peiris, J. S., Couch, R. B., & Yuen, K. Y. (2004). False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide. Journal of Clinical Microbiology, 42(12), 5885-8.
Woo PC, et al. False-positive Results in a Recombinant Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV) Nucleocapsid Enzyme-linked Immunosorbent Assay Due to HCoV-OC43 and HCoV-229E Rectified By Western Blotting With Recombinant SARS-CoV Spike Polypeptide. J Clin Microbiol. 2004;42(12):5885-8. PubMed PMID: 15583332.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide. AU - Woo,Patrick C Y, AU - Lau,Susanna K P, AU - Wong,Beatrice H L, AU - Chan,Kwok-Hung, AU - Hui,Wai-Ting, AU - Kwan,Grace S W, AU - Peiris,J S Malik, AU - Couch,Robert B, AU - Yuen,Kwok-Yung, PY - 2004/12/8/pubmed PY - 2005/2/3/medline PY - 2004/12/8/entrez SP - 5885 EP - 8 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 42 IS - 12 N2 - Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/15583332/False_positive_results_in_a_recombinant_severe_acute_respiratory_syndrome_associated_coronavirus__SARS_CoV__nucleocapsid_enzyme_linked_immunosorbent_assay_due_to_HCoV_OC43_and_HCoV_229E_rectified_by_Western_blotting_with_recombinant_SARS_CoV_spike_polypeptide_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=15583332 DB - PRIME DP - Unbound Medicine ER -