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[Relationship of APOE gene polymorphism with subclasses of serum high density lipoprotein in hyperlipidemia].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Dec; 21(6):615-8.ZY

Abstract

OBJECTIVE

To investigate apolipoprotein E(apoE) polymorphism and its relationship with serum lipids and apolipoprotein, serum high density lipoprotein (HDL) subclasses in patients with hyperlipidemia(HL).

METHODS

APOE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 112 patients with hyperlipidemia and 73 healthy subjects were determined by two-dimensional gel electrophoresis in conjunction with immunodetection method.

RESULTS

APOE3/3 genotypes and allele epsilon3 frequency in HL group and control group were both the highest. In HL group, the genotype of APOE2 had higher serum APOE/CIII ratio and lower HDL3b levels, compared with the genotype of APOE3 (P<0.05). In control group, the genotype of apoE2 had higher serum triglycerides, APOE levels and APOE/CIII ratio, compared with the genotype of APOE3 and APOE4 (P<0.05).

CONCLUSION

Polymorphism of APOE gene may relate to the distribution of HDL particles.

Authors+Show Affiliations

Long SY
Department of Biochemistry, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041 P. R. China.
Zhang XM
No affiliation info available
Fu MD
No affiliation info available
Xu YH
No affiliation info available
Liu BW
No affiliation info available

MeSH

AdultAgedApolipoprotein E2Apolipoprotein E3Apolipoproteins ECholesterolFemaleGene FrequencyGenotypeHumansHyperlipidemiasLipoproteins, HDLMaleMiddle AgedPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthTriglycerides

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15583995

Citation

Long, Shi-yin, et al. "[Relationship of APOE Gene Polymorphism With Subclasses of Serum High Density Lipoprotein in Hyperlipidemia]." Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics, vol. 21, no. 6, 2004, pp. 615-8.
Long SY, Zhang XM, Fu MD, et al. [Relationship of APOE gene polymorphism with subclasses of serum high density lipoprotein in hyperlipidemia]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004;21(6):615-8.
Long, S. Y., Zhang, X. M., Fu, M. D., Xu, Y. H., & Liu, B. W. (2004). [Relationship of APOE gene polymorphism with subclasses of serum high density lipoprotein in hyperlipidemia]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi = Zhonghua Yixue Yichuanxue Zazhi = Chinese Journal of Medical Genetics, 21(6), 615-8.
Long SY, et al. [Relationship of APOE Gene Polymorphism With Subclasses of Serum High Density Lipoprotein in Hyperlipidemia]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004;21(6):615-8. PubMed PMID: 15583995.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Relationship of APOE gene polymorphism with subclasses of serum high density lipoprotein in hyperlipidemia]. AU - Long,Shi-yin, AU - Zhang,Xue-mei, AU - Fu,Ming-de, AU - Xu,Yan-hua, AU - Liu,Bing-wen, PY - 2004/12/8/pubmed PY - 2005/8/18/medline PY - 2004/12/8/entrez SP - 615 EP - 8 JF - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics JO - Zhonghua Yi Xue Yi Chuan Xue Za Zhi VL - 21 IS - 6 N2 - OBJECTIVE: To investigate apolipoprotein E(apoE) polymorphism and its relationship with serum lipids and apolipoprotein, serum high density lipoprotein (HDL) subclasses in patients with hyperlipidemia(HL). METHODS: APOE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 112 patients with hyperlipidemia and 73 healthy subjects were determined by two-dimensional gel electrophoresis in conjunction with immunodetection method. RESULTS: APOE3/3 genotypes and allele epsilon3 frequency in HL group and control group were both the highest. In HL group, the genotype of APOE2 had higher serum APOE/CIII ratio and lower HDL3b levels, compared with the genotype of APOE3 (P<0.05). In control group, the genotype of apoE2 had higher serum triglycerides, APOE levels and APOE/CIII ratio, compared with the genotype of APOE3 and APOE4 (P<0.05). CONCLUSION: Polymorphism of APOE gene may relate to the distribution of HDL particles. SN - 1003-9406 UR - https://www.unboundmedicine.com/medline/citation/15583995/[Relationship_of_APOE_gene_polymorphism_with_subclasses_of_serum_high_density_lipoprotein_in_hyperlipidemia]_ DB - PRIME DP - Unbound Medicine ER -