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Enzymatic degradation of poly(L-lactide) and poly(epsilon-caprolactone) electrospun fibers.
Macromol Biosci 2004; 4(12):1118-25MB

Abstract

Poly(L-lactide) (PLLA) and poly(epsilon-caprolactone) (PCL) ultrafine fibers were prepared by electrospinning. The influence of cationic and anionic surfactants on their enzymatic degradation behavior was investigated by measuring weight loss, molecular weight, crystallinity, and melting temperature of the fibers as a function of degradation time. Under the catalysis of proteinase K, the PLLA fibers containing the anionic surfactant sodium docecyl sulfate (SDS) exhibited a faster degradation rate than those containing cationic surfactant triethylbenzylammonium chloride (TEBAC), indicating that surface electric charge on the fibers is a critical factor for an enzymatic degradation. Similarly, TEBAC-containing PCL fibers exhibited a 47% weight loss within 8.5 h whereas SDS-containing PCL fibers showed little degradation in the presence of lipase PS. By analyzing the charge status of proteinase K and lipase PS under the experimental conditions, the importance of the surface charges of the fibers and their interactions with the charges on the enzymes were revealed. Consequently, a "two-step" degradation mechanism was proposed: (1) the enzyme approaches the fiber surface; (2) the enzyme initiates hydrolysis of the polymer. By means of differential scanning calorimetry and wide-angle X-ray diffraction, the crystallinity and orientation changes in the PLLA and PCL fibers during the enzymatic degradation were investigated, respectively.

Authors+Show Affiliations

State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15586389

Citation

Zeng, Jing, et al. "Enzymatic Degradation of poly(L-lactide) and Poly(epsilon-caprolactone) Electrospun Fibers." Macromolecular Bioscience, vol. 4, no. 12, 2004, pp. 1118-25.
Zeng J, Chen X, Liang Q, et al. Enzymatic degradation of poly(L-lactide) and poly(epsilon-caprolactone) electrospun fibers. Macromol Biosci. 2004;4(12):1118-25.
Zeng, J., Chen, X., Liang, Q., Xu, X., & Jing, X. (2004). Enzymatic degradation of poly(L-lactide) and poly(epsilon-caprolactone) electrospun fibers. Macromolecular Bioscience, 4(12), pp. 1118-25.
Zeng J, et al. Enzymatic Degradation of poly(L-lactide) and Poly(epsilon-caprolactone) Electrospun Fibers. Macromol Biosci. 2004 Dec 15;4(12):1118-25. PubMed PMID: 15586389.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enzymatic degradation of poly(L-lactide) and poly(epsilon-caprolactone) electrospun fibers. AU - Zeng,Jing, AU - Chen,Xuesi, AU - Liang,Qizhi, AU - Xu,Xiuling, AU - Jing,Xiabin, PY - 2004/12/9/pubmed PY - 2005/4/22/medline PY - 2004/12/9/entrez SP - 1118 EP - 25 JF - Macromolecular bioscience JO - Macromol Biosci VL - 4 IS - 12 N2 - Poly(L-lactide) (PLLA) and poly(epsilon-caprolactone) (PCL) ultrafine fibers were prepared by electrospinning. The influence of cationic and anionic surfactants on their enzymatic degradation behavior was investigated by measuring weight loss, molecular weight, crystallinity, and melting temperature of the fibers as a function of degradation time. Under the catalysis of proteinase K, the PLLA fibers containing the anionic surfactant sodium docecyl sulfate (SDS) exhibited a faster degradation rate than those containing cationic surfactant triethylbenzylammonium chloride (TEBAC), indicating that surface electric charge on the fibers is a critical factor for an enzymatic degradation. Similarly, TEBAC-containing PCL fibers exhibited a 47% weight loss within 8.5 h whereas SDS-containing PCL fibers showed little degradation in the presence of lipase PS. By analyzing the charge status of proteinase K and lipase PS under the experimental conditions, the importance of the surface charges of the fibers and their interactions with the charges on the enzymes were revealed. Consequently, a "two-step" degradation mechanism was proposed: (1) the enzyme approaches the fiber surface; (2) the enzyme initiates hydrolysis of the polymer. By means of differential scanning calorimetry and wide-angle X-ray diffraction, the crystallinity and orientation changes in the PLLA and PCL fibers during the enzymatic degradation were investigated, respectively. SN - 1616-5187 UR - https://www.unboundmedicine.com/medline/citation/15586389/Enzymatic_degradation_of_poly_L_lactide__and_poly_epsilon_caprolactone__electrospun_fibers_ L2 - https://doi.org/10.1002/mabi.200400092 DB - PRIME DP - Unbound Medicine ER -