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The effect of connective tissue growth factor on human renal tubular epithelial cell transdifferentiation.

Abstract

To investigate the role of connective tissue growth factor (CTGF) in transdifferentiation of human renal tubular epithelial cell (HKC), in vitro cultured HKC cells were divided into 3 groups: negtive control, low dose CTGF-treated group (rh CTGF, 2.5 ng/ml) and high dose CTGF-treated (rhCTGF, 5.0 ng/ml). Then the expression of alpha-smooth muscle actin (alpha-SMA) were assessed by indirect immuno-fluorescence, and the percentage of alpha-SMA positive cells were assessed by flow cytometry. RT-PCR were also performed to examine the mRNA level of alpha-SMA. Upon the stimulation of different concentrations of rhCTGF, the expression of alpha-SMA were markedly stronger than that in negative controls. The percentages of alpha-SMA positive cells were significantly higher in the stimulated groups than that of negative controls (38.9%, 65.5% vs 2.4%, P<0.01). alpha-SMA mRNA levels were also up-regulated by the stimulation of rhCTGF (P<0.01). These results suggest that CTGF can promote the transdifferentiation of human renal tubular epithelial cells towards myofibroblast (Myo-F).

Authors+Show Affiliations

Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15587396

Citation

Zhang, Chun, et al. "The Effect of Connective Tissue Growth Factor On Human Renal Tubular Epithelial Cell Transdifferentiation." Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban, vol. 24, no. 4, 2004, pp. 350-3.
Zhang C, Zhu Z, Deng A. The effect of connective tissue growth factor on human renal tubular epithelial cell transdifferentiation. J Huazhong Univ Sci Technolog Med Sci. 2004;24(4):350-3.
Zhang, C., Zhu, Z., & Deng, A. (2004). The effect of connective tissue growth factor on human renal tubular epithelial cell transdifferentiation. Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban, 24(4), 350-3.
Zhang C, Zhu Z, Deng A. The Effect of Connective Tissue Growth Factor On Human Renal Tubular Epithelial Cell Transdifferentiation. J Huazhong Univ Sci Technolog Med Sci. 2004;24(4):350-3. PubMed PMID: 15587396.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of connective tissue growth factor on human renal tubular epithelial cell transdifferentiation. AU - Zhang,Chun, AU - Zhu,Zhonghua, AU - Deng,Anguo, PY - 2004/12/14/pubmed PY - 2005/8/27/medline PY - 2004/12/14/entrez SP - 350 EP - 3 JF - Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban JO - J Huazhong Univ Sci Technolog Med Sci VL - 24 IS - 4 N2 - To investigate the role of connective tissue growth factor (CTGF) in transdifferentiation of human renal tubular epithelial cell (HKC), in vitro cultured HKC cells were divided into 3 groups: negtive control, low dose CTGF-treated group (rh CTGF, 2.5 ng/ml) and high dose CTGF-treated (rhCTGF, 5.0 ng/ml). Then the expression of alpha-smooth muscle actin (alpha-SMA) were assessed by indirect immuno-fluorescence, and the percentage of alpha-SMA positive cells were assessed by flow cytometry. RT-PCR were also performed to examine the mRNA level of alpha-SMA. Upon the stimulation of different concentrations of rhCTGF, the expression of alpha-SMA were markedly stronger than that in negative controls. The percentages of alpha-SMA positive cells were significantly higher in the stimulated groups than that of negative controls (38.9%, 65.5% vs 2.4%, P<0.01). alpha-SMA mRNA levels were also up-regulated by the stimulation of rhCTGF (P<0.01). These results suggest that CTGF can promote the transdifferentiation of human renal tubular epithelial cells towards myofibroblast (Myo-F). SN - 1672-0733 UR - https://www.unboundmedicine.com/medline/citation/15587396/The_effect_of_connective_tissue_growth_factor_on_human_renal_tubular_epithelial_cell_transdifferentiation_ DB - PRIME DP - Unbound Medicine ER -