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Antioxidant effect of Inonotus obliquus.
J Ethnopharmacol. 2005 Jan 04; 96(1-2):79-85.JE

Abstract

The mushroom Inonotus obliquus (Fr.) Pilát (Hymenochaetaceae), has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. The purpose of this study was to elucidate the antioxidant capacities of Inonotus obliquus. Four extracts from the fungus were evaluated for antioxidant activity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, and peroxyl radicals. The polyphenolic extract had a strong antioxidant activity, and the extract containing triterpenoids and steroids presented a relatively strong antioxidant effect. The polysaccharide extract, however, was inactive. The protective effects of these four extracts were assessed against hydrogen peroxide-induced oxidative stress using a human keratinocyte cell line, HaCaT. Our results show that the polyphenolic extract protected these cells against hydrogen peroxide-induced oxidative stress, while the polysaccharide, triterpenoid and steroid extracts were ineffective. Additionally, the remnant polyphenolic and low molecular weight polysaccharide extracts showed a weakly protective effect at a concentration of 50 microg/ml. Our results indicate that Inonotus obliquus has the capacity to scavenge free radicals at concentrations higher than 5 microg/ml and that the polyphenolic extract can protect cells against oxidative stress.

Authors+Show Affiliations

Department of Dermatology, Seoul National University, Bundang Hospital, 300 Gumi-Dong, Bundang-Gu, Seongnam-Si, Kyoungki-Do 463-707, Republic of Korea.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15588653

Citation

Cui, Yong, et al. "Antioxidant Effect of Inonotus Obliquus." Journal of Ethnopharmacology, vol. 96, no. 1-2, 2005, pp. 79-85.
Cui Y, Kim DS, Park KC. Antioxidant effect of Inonotus obliquus. J Ethnopharmacol. 2005;96(1-2):79-85.
Cui, Y., Kim, D. S., & Park, K. C. (2005). Antioxidant effect of Inonotus obliquus. Journal of Ethnopharmacology, 96(1-2), 79-85.
Cui Y, Kim DS, Park KC. Antioxidant Effect of Inonotus Obliquus. J Ethnopharmacol. 2005 Jan 4;96(1-2):79-85. PubMed PMID: 15588653.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant effect of Inonotus obliquus. AU - Cui,Yong, AU - Kim,Dong-Seok, AU - Park,Kyoung-Chan, PY - 2003/12/10/received PY - 2004/08/18/revised PY - 2004/08/19/accepted PY - 2004/12/14/pubmed PY - 2005/6/14/medline PY - 2004/12/14/entrez SP - 79 EP - 85 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 96 IS - 1-2 N2 - The mushroom Inonotus obliquus (Fr.) Pilát (Hymenochaetaceae), has been widely used as a folk medicine in Russia, Poland and most of the Baltic countries. The purpose of this study was to elucidate the antioxidant capacities of Inonotus obliquus. Four extracts from the fungus were evaluated for antioxidant activity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, and peroxyl radicals. The polyphenolic extract had a strong antioxidant activity, and the extract containing triterpenoids and steroids presented a relatively strong antioxidant effect. The polysaccharide extract, however, was inactive. The protective effects of these four extracts were assessed against hydrogen peroxide-induced oxidative stress using a human keratinocyte cell line, HaCaT. Our results show that the polyphenolic extract protected these cells against hydrogen peroxide-induced oxidative stress, while the polysaccharide, triterpenoid and steroid extracts were ineffective. Additionally, the remnant polyphenolic and low molecular weight polysaccharide extracts showed a weakly protective effect at a concentration of 50 microg/ml. Our results indicate that Inonotus obliquus has the capacity to scavenge free radicals at concentrations higher than 5 microg/ml and that the polyphenolic extract can protect cells against oxidative stress. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/15588653/Antioxidant_effect_of_Inonotus_obliquus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(04)00457-X DB - PRIME DP - Unbound Medicine ER -