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High temporal resolution for in vivo monitoring of neurotransmitters in awake epileptic rats using brain microdialysis and capillary electrophoresis with laser-induced fluorescence detection.
J Neurosci Methods. 2004 Dec 30; 140(1-2):29-38.JN

Abstract

A method for high temporal resolution monitoring of five neurotransmitters, dopamine (DA), noradrenaline (NA), gamma-aminobutyric acid (GABA), glutamate (Glu), l-aspartate (L-Asp), in freely-moving rats using microdialysis and capillary electrophoresis with laser-induced fluorescence detection (CE-LIFD) was developed. An on-line device, including microdialysis and derivatization with naphthalene-2,3-dicarboxaldehyde, mixes the dialysate with derivatization reagents directly in the collection tube, i.e. with no reactor. Thereafter, collected derivatized samples are analyzed off-line with an automated CE system coupled to a LIFD using a 442 nm excitation. The sampling time was limited by the minimal volume required for the analysis by the automated CE system used: neurotransmitters could be determined in 667 nl dialysates (940 nl after derivatization), i.e. in samples collected every 20 s with a flow rate of 2 microl/min. The detection limits at the dialysis probe were 3 x 10(-9), 1 x 10(-9), 1.9 x 10(-8), 4.2 x 10(-7), 2.1 x 10(-7) mol/l for DA, NA, GABA, Glu and L-Asp, respectively. The protocol was validated using in vitro/in vivo tests and the performances--repeatability, linearity, characteristics of the probes--were determined. Finally, the high temporal resolution allowed the simultaneous monitoring of these neurotransmitters in rats with genetic absence epilepsy and revealed, for the first time, increases in GABA concentrations concomitantly with the seizures, detected when our new microdialysis method was combined to electroencephalographic recordings.

Authors+Show Affiliations

Laboratoire de Neuropharmacologie et Neurochimie, INSERM U512, Institut Fédératif des Neurosciences de Lyon (IFR 19), Université Claude Bernard--Lyon I, 8 Avenue Rockefeller, 69373 Lyon 08, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15589331

Citation

Parrot, Sandrine, et al. "High Temporal Resolution for in Vivo Monitoring of Neurotransmitters in Awake Epileptic Rats Using Brain Microdialysis and Capillary Electrophoresis With Laser-induced Fluorescence Detection." Journal of Neuroscience Methods, vol. 140, no. 1-2, 2004, pp. 29-38.
Parrot S, Sauvinet V, Riban V, et al. High temporal resolution for in vivo monitoring of neurotransmitters in awake epileptic rats using brain microdialysis and capillary electrophoresis with laser-induced fluorescence detection. J Neurosci Methods. 2004;140(1-2):29-38.
Parrot, S., Sauvinet, V., Riban, V., Depaulis, A., Renaud, B., & Denoroy, L. (2004). High temporal resolution for in vivo monitoring of neurotransmitters in awake epileptic rats using brain microdialysis and capillary electrophoresis with laser-induced fluorescence detection. Journal of Neuroscience Methods, 140(1-2), 29-38.
Parrot S, et al. High Temporal Resolution for in Vivo Monitoring of Neurotransmitters in Awake Epileptic Rats Using Brain Microdialysis and Capillary Electrophoresis With Laser-induced Fluorescence Detection. J Neurosci Methods. 2004 Dec 30;140(1-2):29-38. PubMed PMID: 15589331.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High temporal resolution for in vivo monitoring of neurotransmitters in awake epileptic rats using brain microdialysis and capillary electrophoresis with laser-induced fluorescence detection. AU - Parrot,Sandrine, AU - Sauvinet,Valérie, AU - Riban,Véronique, AU - Depaulis,Antoine, AU - Renaud,Bernard, AU - Denoroy,Luc, PY - 2003/10/30/received PY - 2004/03/22/accepted PY - 2004/12/14/pubmed PY - 2005/4/13/medline PY - 2004/12/14/entrez SP - 29 EP - 38 JF - Journal of neuroscience methods JO - J Neurosci Methods VL - 140 IS - 1-2 N2 - A method for high temporal resolution monitoring of five neurotransmitters, dopamine (DA), noradrenaline (NA), gamma-aminobutyric acid (GABA), glutamate (Glu), l-aspartate (L-Asp), in freely-moving rats using microdialysis and capillary electrophoresis with laser-induced fluorescence detection (CE-LIFD) was developed. An on-line device, including microdialysis and derivatization with naphthalene-2,3-dicarboxaldehyde, mixes the dialysate with derivatization reagents directly in the collection tube, i.e. with no reactor. Thereafter, collected derivatized samples are analyzed off-line with an automated CE system coupled to a LIFD using a 442 nm excitation. The sampling time was limited by the minimal volume required for the analysis by the automated CE system used: neurotransmitters could be determined in 667 nl dialysates (940 nl after derivatization), i.e. in samples collected every 20 s with a flow rate of 2 microl/min. The detection limits at the dialysis probe were 3 x 10(-9), 1 x 10(-9), 1.9 x 10(-8), 4.2 x 10(-7), 2.1 x 10(-7) mol/l for DA, NA, GABA, Glu and L-Asp, respectively. The protocol was validated using in vitro/in vivo tests and the performances--repeatability, linearity, characteristics of the probes--were determined. Finally, the high temporal resolution allowed the simultaneous monitoring of these neurotransmitters in rats with genetic absence epilepsy and revealed, for the first time, increases in GABA concentrations concomitantly with the seizures, detected when our new microdialysis method was combined to electroencephalographic recordings. SN - 0165-0270 UR - https://www.unboundmedicine.com/medline/citation/15589331/High_temporal_resolution_for_in_vivo_monitoring_of_neurotransmitters_in_awake_epileptic_rats_using_brain_microdialysis_and_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-0270(04)00278-X DB - PRIME DP - Unbound Medicine ER -