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Inhibitory effect of luteolin on TNF-alpha-induced IL-8 production in human colon epithelial cells.
Int Immunopharmacol. 2005 Jan; 5(1):209-17.II

Abstract

Interleukin (IL)-8 plays a central role in the initiation and maintenance of inflammatory responses in the inflammatory bowel disease. The proinflammatory cytokine-mediated production of IL-8 requires activation of various kinases, which leads to the IkappaB degradation and NF-kappaB activation. In this study, we investigated the role of luteolin, a major flavonoid of Lonicera japonica, on TNF-alpha-induced IL-8 production in human colonic epithelial cells. HT29 cells were stimulated with TNF-alpha in the presence or absence of luteolin. IL-8 production was measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, and the mitogen-activated protein kinases (MAPKs) activation and IkappaB degradation were determined by Western blot analysis. NF-kappaB activation was assessed by the electrophoretic motility shift assay (EMSA). Luteolin suppressed TNF-alpha-induced IL-8 production in dose-dependent manner. In addition, luteolin inhibited TNF-alpha-induced phosphorylation of p38 MAPK and extracellular-regulated kinases (ERK), IkappaB degradation, and NF-kappaB activation. These results suggest that luteolin has the inhibitory effects on TNF-alpha-induced IL-8 production in the intestinal epithelial cells through blockade in the phosphorylation of MAPKs, following IkappaB degradation and NF-kappaB activation.

Authors+Show Affiliations

Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, 570-749, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15589482

Citation

Kim, Jin-A, et al. "Inhibitory Effect of Luteolin On TNF-alpha-induced IL-8 Production in Human Colon Epithelial Cells." International Immunopharmacology, vol. 5, no. 1, 2005, pp. 209-17.
Kim JA, Kim DK, Kang OH, et al. Inhibitory effect of luteolin on TNF-alpha-induced IL-8 production in human colon epithelial cells. Int Immunopharmacol. 2005;5(1):209-17.
Kim, J. A., Kim, D. K., Kang, O. H., Choi, Y. A., Park, H. J., Choi, S. C., Kim, T. H., Yun, K. J., Nah, Y. H., & Lee, Y. M. (2005). Inhibitory effect of luteolin on TNF-alpha-induced IL-8 production in human colon epithelial cells. International Immunopharmacology, 5(1), 209-17.
Kim JA, et al. Inhibitory Effect of Luteolin On TNF-alpha-induced IL-8 Production in Human Colon Epithelial Cells. Int Immunopharmacol. 2005;5(1):209-17. PubMed PMID: 15589482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of luteolin on TNF-alpha-induced IL-8 production in human colon epithelial cells. AU - Kim,Jin-A, AU - Kim,Dae-Ki, AU - Kang,Ok-Hwa, AU - Choi,Yeon-A, AU - Park,Hye-Jung, AU - Choi,Suck-Chei, AU - Kim,Tae-Hyun, AU - Yun,Ki-Jung, AU - Nah,Yong-Ho, AU - Lee,Young-Mi, PY - 2004/07/12/received PY - 2004/09/11/revised PY - 2004/09/14/accepted PY - 2004/12/14/pubmed PY - 2005/8/9/medline PY - 2004/12/14/entrez SP - 209 EP - 17 JF - International immunopharmacology JO - Int Immunopharmacol VL - 5 IS - 1 N2 - Interleukin (IL)-8 plays a central role in the initiation and maintenance of inflammatory responses in the inflammatory bowel disease. The proinflammatory cytokine-mediated production of IL-8 requires activation of various kinases, which leads to the IkappaB degradation and NF-kappaB activation. In this study, we investigated the role of luteolin, a major flavonoid of Lonicera japonica, on TNF-alpha-induced IL-8 production in human colonic epithelial cells. HT29 cells were stimulated with TNF-alpha in the presence or absence of luteolin. IL-8 production was measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis, and the mitogen-activated protein kinases (MAPKs) activation and IkappaB degradation were determined by Western blot analysis. NF-kappaB activation was assessed by the electrophoretic motility shift assay (EMSA). Luteolin suppressed TNF-alpha-induced IL-8 production in dose-dependent manner. In addition, luteolin inhibited TNF-alpha-induced phosphorylation of p38 MAPK and extracellular-regulated kinases (ERK), IkappaB degradation, and NF-kappaB activation. These results suggest that luteolin has the inhibitory effects on TNF-alpha-induced IL-8 production in the intestinal epithelial cells through blockade in the phosphorylation of MAPKs, following IkappaB degradation and NF-kappaB activation. SN - 1567-5769 UR - https://www.unboundmedicine.com/medline/citation/15589482/Inhibitory_effect_of_luteolin_on_TNF_alpha_induced_IL_8_production_in_human_colon_epithelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-5769(04)00313-3 DB - PRIME DP - Unbound Medicine ER -