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Antibody response in patients with cutaneous leishmaniasis infected by Leishmania (Viannia) braziliensis or Leishmania (Viannia) guyanensis in Brazil.
Acta Trop. 2005 Jan; 93(1):49-56.AT

Abstract

The antibody response against Leishmania (Leishmania) amazonensis crude antigen was measured through the indirect immunofluorescent assay (IFA) and the immunoenzymatic assay (ELISA) in 114 patients with cutaneous leishmaniasis (CL) in Brazil. Fifty-four patients were infected by Leishmania (Viannia) braziliensis, and 60 patients had L. (V.) guyanensis infection. Patients were comparable by age, sex, disease duration and the Montenegro skin test diameter. L. (V.) braziliensis-infected patients showed significant lower number of ulcerated lesions, greater ulcerated area and higher proportion of lymph node enlargement. Sensitivity of IFA was 79.6% (95% CI 66.1-88.9) and 71.7% (95% CI 58.4-82.2) for L. (V.) braziliensis and L. (V.) guyanensis-infected patients, respectively (P=0.324). Sensitivity of ELISA was 98.2% (95% CI 88.8-99.9) and 85.0% (95% CI 72.9-92.5) for L. (V.) braziliensis and L. (V.) guyanensis-infected patients, respectively (P=0.018). Significant differences were observed in the magnitude of the antibody response before treatment with higher levels detected in L. (V.) braziliensis-infected patients by both serologic techniques. Eighty-four patients had serologic evaluations before and 12 weeks after treatment with meglumine antimoniate, 20 mg/kg/day for 20 days. Significant lower optic density values were observed after treatment with both species independent of cure or failure. Our data showed that L. (V.) braziliensis induces a higher antibody response against L. (L.) amazonensis antigens than L. (V.) guyanensis and that down-modulation of the antibody response occurs shortly during disease evolution after treatment. Moreover the data support the use of ELISA as a better tool for detection of antibodies in CL.

Authors+Show Affiliations

Núcleo de Medicina Tropical, Faculdade de Medicina, Universidade de Brasília, Caixa Postal 04517, Campus Universitário, Asa Norte, Brasília, Distrito Federal 70919-970, Brazil. gromero@unb.brNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15589797

Citation

Romero, Gustavo Adolfo Sierra, et al. "Antibody Response in Patients With Cutaneous Leishmaniasis Infected By Leishmania (Viannia) Braziliensis or Leishmania (Viannia) Guyanensis in Brazil." Acta Tropica, vol. 93, no. 1, 2005, pp. 49-56.
Romero GA, de la Glória Orge Orge M, de Farias Guerra MV, et al. Antibody response in patients with cutaneous leishmaniasis infected by Leishmania (Viannia) braziliensis or Leishmania (Viannia) guyanensis in Brazil. Acta Trop. 2005;93(1):49-56.
Romero, G. A., de la Glória Orge Orge, M., de Farias Guerra, M. V., Paes, M. G., de Oliveira Macêdo, V., & de Carvalho, E. M. (2005). Antibody response in patients with cutaneous leishmaniasis infected by Leishmania (Viannia) braziliensis or Leishmania (Viannia) guyanensis in Brazil. Acta Tropica, 93(1), 49-56.
Romero GA, et al. Antibody Response in Patients With Cutaneous Leishmaniasis Infected By Leishmania (Viannia) Braziliensis or Leishmania (Viannia) Guyanensis in Brazil. Acta Trop. 2005;93(1):49-56. PubMed PMID: 15589797.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibody response in patients with cutaneous leishmaniasis infected by Leishmania (Viannia) braziliensis or Leishmania (Viannia) guyanensis in Brazil. AU - Romero,Gustavo Adolfo Sierra, AU - de la Glória Orge Orge,Maria, AU - de Farias Guerra,Marcus Vinitius, AU - Paes,Marcilene Gomes, AU - de Oliveira Macêdo,Vanize, AU - de Carvalho,Edgar M, PY - 2004/05/04/received PY - 2004/08/31/revised PY - 2004/09/09/accepted PY - 2004/12/14/pubmed PY - 2005/3/9/medline PY - 2004/12/14/entrez SP - 49 EP - 56 JF - Acta tropica JO - Acta Trop VL - 93 IS - 1 N2 - The antibody response against Leishmania (Leishmania) amazonensis crude antigen was measured through the indirect immunofluorescent assay (IFA) and the immunoenzymatic assay (ELISA) in 114 patients with cutaneous leishmaniasis (CL) in Brazil. Fifty-four patients were infected by Leishmania (Viannia) braziliensis, and 60 patients had L. (V.) guyanensis infection. Patients were comparable by age, sex, disease duration and the Montenegro skin test diameter. L. (V.) braziliensis-infected patients showed significant lower number of ulcerated lesions, greater ulcerated area and higher proportion of lymph node enlargement. Sensitivity of IFA was 79.6% (95% CI 66.1-88.9) and 71.7% (95% CI 58.4-82.2) for L. (V.) braziliensis and L. (V.) guyanensis-infected patients, respectively (P=0.324). Sensitivity of ELISA was 98.2% (95% CI 88.8-99.9) and 85.0% (95% CI 72.9-92.5) for L. (V.) braziliensis and L. (V.) guyanensis-infected patients, respectively (P=0.018). Significant differences were observed in the magnitude of the antibody response before treatment with higher levels detected in L. (V.) braziliensis-infected patients by both serologic techniques. Eighty-four patients had serologic evaluations before and 12 weeks after treatment with meglumine antimoniate, 20 mg/kg/day for 20 days. Significant lower optic density values were observed after treatment with both species independent of cure or failure. Our data showed that L. (V.) braziliensis induces a higher antibody response against L. (L.) amazonensis antigens than L. (V.) guyanensis and that down-modulation of the antibody response occurs shortly during disease evolution after treatment. Moreover the data support the use of ELISA as a better tool for detection of antibodies in CL. SN - 0001-706X UR - https://www.unboundmedicine.com/medline/citation/15589797/Antibody_response_in_patients_with_cutaneous_leishmaniasis_infected_by_Leishmania__Viannia__braziliensis_or_Leishmania__Viannia__guyanensis_in_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(04)00208-6 DB - PRIME DP - Unbound Medicine ER -