Tags

Type your tag names separated by a space and hit enter

Mast cells in citric acid-induced cough of guinea pigs.
Toxicol Appl Pharmacol. 2005 Jan 01; 202(1):18-24.TA

Abstract

It was demonstrated previously that mast cells play an important role in citric acid (CA)-induced airway constriction. To investigate the role of mast cells in CA-induced cough, three experiments were carried out in this study. In the first experiment, 59 guinea pigs were employed and we used compound 48/80 to deplete mast cells, cromolyn sodium to stabilize mast cells, MK-886 to inhibit leukotriene synthesis, pyrilamine to antagonize histamine H(1) receptor, methysergide to antagonize serotonin receptor, and indomethacin to inhibit cyclooxygenase. In the second experiment, 56 compound 48/80-pretreated animals were divided into two parts; the first one was used to test the role of exogenous leukotriene (LT) C(4), while the second one to test the role of exogenous histamine in CA-induced cough. Each animal with one of the above pretreatments was exposed sequentially to saline (baseline) and CA (0.6 M) aerosol, each for 3 min. Then, cough was recorded for 12 min using a barometric body plethysmograph. In the third experiment, the activation of mast cells upon CA inhalation was investigated by determining arterial plasma histamine concentration in 17 animals. Exposure to CA induced a marked increase in cough number. Compound 48/80, cromolyn sodium, MK-886 and pyrilamine, but not indomethacin or methysergide, significantly attenuated CA-induced cough. Injection of LTC(4) or histamine caused a significant increase in CA-induced cough in compound 48/80-pretreated animals. In addition, CA inhalation caused significant increase in plasma histamine concentration, which was blocked by compound 48/80 pretreatment. These results suggest that mast cells play an important role in CA aerosol inhalation-induced cough via perhaps mediators LTs and histamine.

Authors+Show Affiliations

Department of Physiology, National Taiwan University College of Medicine, Taipei 100, Taiwan. tiger@ha.mc.ntu.edu.twNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15589973

Citation

Lai, Yih-Loong, and Tai-Yin Lin. "Mast Cells in Citric Acid-induced Cough of Guinea Pigs." Toxicology and Applied Pharmacology, vol. 202, no. 1, 2005, pp. 18-24.
Lai YL, Lin TY. Mast cells in citric acid-induced cough of guinea pigs. Toxicol Appl Pharmacol. 2005;202(1):18-24.
Lai, Y. L., & Lin, T. Y. (2005). Mast cells in citric acid-induced cough of guinea pigs. Toxicology and Applied Pharmacology, 202(1), 18-24.
Lai YL, Lin TY. Mast Cells in Citric Acid-induced Cough of Guinea Pigs. Toxicol Appl Pharmacol. 2005 Jan 1;202(1):18-24. PubMed PMID: 15589973.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mast cells in citric acid-induced cough of guinea pigs. AU - Lai,Yih-Loong, AU - Lin,Tai-Yin, PY - 2004/03/12/received PY - 2004/05/19/revised PY - 2004/05/21/accepted PY - 2004/12/14/pubmed PY - 2005/2/4/medline PY - 2004/12/14/entrez SP - 18 EP - 24 JF - Toxicology and applied pharmacology JO - Toxicol Appl Pharmacol VL - 202 IS - 1 N2 - It was demonstrated previously that mast cells play an important role in citric acid (CA)-induced airway constriction. To investigate the role of mast cells in CA-induced cough, three experiments were carried out in this study. In the first experiment, 59 guinea pigs were employed and we used compound 48/80 to deplete mast cells, cromolyn sodium to stabilize mast cells, MK-886 to inhibit leukotriene synthesis, pyrilamine to antagonize histamine H(1) receptor, methysergide to antagonize serotonin receptor, and indomethacin to inhibit cyclooxygenase. In the second experiment, 56 compound 48/80-pretreated animals were divided into two parts; the first one was used to test the role of exogenous leukotriene (LT) C(4), while the second one to test the role of exogenous histamine in CA-induced cough. Each animal with one of the above pretreatments was exposed sequentially to saline (baseline) and CA (0.6 M) aerosol, each for 3 min. Then, cough was recorded for 12 min using a barometric body plethysmograph. In the third experiment, the activation of mast cells upon CA inhalation was investigated by determining arterial plasma histamine concentration in 17 animals. Exposure to CA induced a marked increase in cough number. Compound 48/80, cromolyn sodium, MK-886 and pyrilamine, but not indomethacin or methysergide, significantly attenuated CA-induced cough. Injection of LTC(4) or histamine caused a significant increase in CA-induced cough in compound 48/80-pretreated animals. In addition, CA inhalation caused significant increase in plasma histamine concentration, which was blocked by compound 48/80 pretreatment. These results suggest that mast cells play an important role in CA aerosol inhalation-induced cough via perhaps mediators LTs and histamine. SN - 0041-008X UR - https://www.unboundmedicine.com/medline/citation/15589973/Mast_cells_in_citric_acid_induced_cough_of_guinea_pigs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(04)00285-6 DB - PRIME DP - Unbound Medicine ER -