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Neuropeptide Y-Y1 receptor agonist worsens while antagonist improves survival of cultured Y1-expressing neuronal cells following oxygen and glucose deprivation.
J Biomed Sci. 2004 Nov-Dec; 11(6):781-8.JB

Abstract

In this in vitro study, we investigated the influence of neuropeptide Y (NPY) Y1 receptor activation or inhibition on the viability of cultured neuronal or glial cells following oxygen glucose deprivation (OGD). Viability of cultured cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. When compared to the vehicle-treated control group, treatment with NPY or [Leu31,Pro34]-NPY (Y1 agonist) reduced viability of cultured SK-N-MC (Y1-expressing) human neuronal cells at 24 h after 1 h of OGD, while BIBP3226 (Y1 antagonist) improved viability. Except at the highest concentration of NPY used in the study, treatment with NPY or NPY3-36 (Y2 agonist) did not influence viability of cultured SH-SY5Y (Y2-expressing) human neuronal cells at 24 h after 1 h of OGD. In addition, treatment with NPY, [Leu31,Pro34]-NPY, NPY3-36, or BIBP3226 did not affect viability of cultured primary astrocytes at 24 h after 4 h of OGD. The present results agree with those of a recent in vivo study. Activation of NPY-Y1 receptors may mediate ischemic pathophysiological processes, and inhibiting the Y1 receptors may be protective. The combination of OGD and cultured neuronal cells may be useful in future studies on the neuroprotective and harmful mechanisms of NPY-Y1 receptor inhibition and activation during ischemia, respectively.

Authors+Show Affiliations

Division of Neurology, University Department of Medicine, University of Hong Kong, Hong Kong.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15591775

Citation

Chen, Shao Hua, and Raymond Tak Fai Cheung. "Neuropeptide Y-Y1 Receptor Agonist Worsens While Antagonist Improves Survival of Cultured Y1-expressing Neuronal Cells Following Oxygen and Glucose Deprivation." Journal of Biomedical Science, vol. 11, no. 6, 2004, pp. 781-8.
Chen SH, Cheung RT. Neuropeptide Y-Y1 receptor agonist worsens while antagonist improves survival of cultured Y1-expressing neuronal cells following oxygen and glucose deprivation. J Biomed Sci. 2004;11(6):781-8.
Chen, S. H., & Cheung, R. T. (2004). Neuropeptide Y-Y1 receptor agonist worsens while antagonist improves survival of cultured Y1-expressing neuronal cells following oxygen and glucose deprivation. Journal of Biomedical Science, 11(6), 781-8.
Chen SH, Cheung RT. Neuropeptide Y-Y1 Receptor Agonist Worsens While Antagonist Improves Survival of Cultured Y1-expressing Neuronal Cells Following Oxygen and Glucose Deprivation. J Biomed Sci. 2004 Nov-Dec;11(6):781-8. PubMed PMID: 15591775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuropeptide Y-Y1 receptor agonist worsens while antagonist improves survival of cultured Y1-expressing neuronal cells following oxygen and glucose deprivation. AU - Chen,Shao Hua, AU - Cheung,Raymond Tak Fai, PY - 2004/04/20/received PY - 2004/07/12/accepted PY - 2004/12/14/pubmed PY - 2005/5/25/medline PY - 2004/12/14/entrez SP - 781 EP - 8 JF - Journal of biomedical science JO - J. Biomed. Sci. VL - 11 IS - 6 N2 - In this in vitro study, we investigated the influence of neuropeptide Y (NPY) Y1 receptor activation or inhibition on the viability of cultured neuronal or glial cells following oxygen glucose deprivation (OGD). Viability of cultured cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. When compared to the vehicle-treated control group, treatment with NPY or [Leu31,Pro34]-NPY (Y1 agonist) reduced viability of cultured SK-N-MC (Y1-expressing) human neuronal cells at 24 h after 1 h of OGD, while BIBP3226 (Y1 antagonist) improved viability. Except at the highest concentration of NPY used in the study, treatment with NPY or NPY3-36 (Y2 agonist) did not influence viability of cultured SH-SY5Y (Y2-expressing) human neuronal cells at 24 h after 1 h of OGD. In addition, treatment with NPY, [Leu31,Pro34]-NPY, NPY3-36, or BIBP3226 did not affect viability of cultured primary astrocytes at 24 h after 4 h of OGD. The present results agree with those of a recent in vivo study. Activation of NPY-Y1 receptors may mediate ischemic pathophysiological processes, and inhibiting the Y1 receptors may be protective. The combination of OGD and cultured neuronal cells may be useful in future studies on the neuroprotective and harmful mechanisms of NPY-Y1 receptor inhibition and activation during ischemia, respectively. SN - 1021-7770 UR - https://www.unboundmedicine.com/medline/citation/15591775/Neuropeptide_Y_Y1_receptor_agonist_worsens_while_antagonist_improves_survival_of_cultured_Y1_expressing_neuronal_cells_following_oxygen_and_glucose_deprivation_ DB - PRIME DP - Unbound Medicine ER -